Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity
Ankylosing spondylitis (AS) is a chronic inflammatory disease that results in severe pain and stiffness in the joints. The causes and pathophysiology of AS are still largely unknown. The lncRNA H19 plays key roles in the pathogenesis of AS by mediating inflammatory progression by acting in the axis...
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MDPI AG
2023-05-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/13/6/914 |
| _version_ | 1797593960507506688 |
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| author | Marwa M. Esawy Amany M. Ebaid Amir Abd-elhameed Felwa A. Thagfan Murad A. Mubaraki Ahmed S. Alazzouni Mohamed A. Dkhil Marwa A. Shabana |
| author_facet | Marwa M. Esawy Amany M. Ebaid Amir Abd-elhameed Felwa A. Thagfan Murad A. Mubaraki Ahmed S. Alazzouni Mohamed A. Dkhil Marwa A. Shabana |
| author_sort | Marwa M. Esawy |
| collection | DOAJ |
| description | Ankylosing spondylitis (AS) is a chronic inflammatory disease that results in severe pain and stiffness in the joints. The causes and pathophysiology of AS are still largely unknown. The lncRNA H19 plays key roles in the pathogenesis of AS by mediating inflammatory progression by acting in the axis of IL-17A/IL-23. The aims of this study were determining the role of lncRNA H19 in AS and assessing its clinical correlation. A case–control study was conducted and qRT-PCR was utilized to measure H19 expression. Comparing AS cases to healthy controls, it was found that H19 expression was significantly upregulated. For AS prediction, H19 demonstrated a 81.1% sensitivity, 100% specificity, and 90.6% diagnostic accuracy at a lncRNA H19 expression value of 1.41. lncRNA H19 had a significantly positive correlation with AS activity, MRI results, and inflammatory markers. lncRNA H19 seemed to be an independent predictor of AS (adjusted OR of 211 (95% CI: 4.7–939; <i>p</i> = 0.025)). After 3 months of clinical follow-up, seventeen patients (32.1%) showed minimal clinical improvement and fifteen patients (28.3%) showed major improvement. AS activity scores were significantly decreased in patients with high H19 expression. A significantly elevated lncRNA H19 expression was observed in AS cases compared with that in healthy controls. These results suggest that upregulation of lncRNA H19 expression may be involved in the pathogenesis of AS. The expression of the lncRNA H19 is related to the duration and activity of the disease. LncRNA H19 expression seems to be an independent predictor of AS. |
| first_indexed | 2024-03-11T02:16:55Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-11T02:16:55Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-f3a8d225b76a4d90ae5b28c5197c4a9b2023-11-18T11:10:30ZengMDPI AGJournal of Personalized Medicine2075-44262023-05-0113691410.3390/jpm13060914Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease ActivityMarwa M. Esawy0Amany M. Ebaid1Amir Abd-elhameed2Felwa A. Thagfan3Murad A. Mubaraki4Ahmed S. Alazzouni5Mohamed A. Dkhil6Marwa A. Shabana7Clinical Pathology Department, Faculty of Human Medicine, Zagazig University, Zagazig 44519, EgyptRheumatology and Rehabilitation Department, Faculty of Human Medicine, Zagazig University, Zagazig 44519, EgyptInternal Medicine Department, Faculty of Human Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaClinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi ArabiaDepartment of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, EgyptDepartment of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, EgyptClinical Pathology Department, Faculty of Human Medicine, Zagazig University, Zagazig 44519, EgyptAnkylosing spondylitis (AS) is a chronic inflammatory disease that results in severe pain and stiffness in the joints. The causes and pathophysiology of AS are still largely unknown. The lncRNA H19 plays key roles in the pathogenesis of AS by mediating inflammatory progression by acting in the axis of IL-17A/IL-23. The aims of this study were determining the role of lncRNA H19 in AS and assessing its clinical correlation. A case–control study was conducted and qRT-PCR was utilized to measure H19 expression. Comparing AS cases to healthy controls, it was found that H19 expression was significantly upregulated. For AS prediction, H19 demonstrated a 81.1% sensitivity, 100% specificity, and 90.6% diagnostic accuracy at a lncRNA H19 expression value of 1.41. lncRNA H19 had a significantly positive correlation with AS activity, MRI results, and inflammatory markers. lncRNA H19 seemed to be an independent predictor of AS (adjusted OR of 211 (95% CI: 4.7–939; <i>p</i> = 0.025)). After 3 months of clinical follow-up, seventeen patients (32.1%) showed minimal clinical improvement and fifteen patients (28.3%) showed major improvement. AS activity scores were significantly decreased in patients with high H19 expression. A significantly elevated lncRNA H19 expression was observed in AS cases compared with that in healthy controls. These results suggest that upregulation of lncRNA H19 expression may be involved in the pathogenesis of AS. The expression of the lncRNA H19 is related to the duration and activity of the disease. LncRNA H19 expression seems to be an independent predictor of AS.https://www.mdpi.com/2075-4426/13/6/914lncRNA H19ankylosing spondylitisdisease activitypredictor |
| spellingShingle | Marwa M. Esawy Amany M. Ebaid Amir Abd-elhameed Felwa A. Thagfan Murad A. Mubaraki Ahmed S. Alazzouni Mohamed A. Dkhil Marwa A. Shabana Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity Journal of Personalized Medicine lncRNA H19 ankylosing spondylitis disease activity predictor |
| title | Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity |
| title_full | Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity |
| title_fullStr | Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity |
| title_full_unstemmed | Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity |
| title_short | Assessment of Circulating lncRNA H19 in Ankylosing Spondylitis Patients and Its Correlation with Disease Activity |
| title_sort | assessment of circulating lncrna h19 in ankylosing spondylitis patients and its correlation with disease activity |
| topic | lncRNA H19 ankylosing spondylitis disease activity predictor |
| url | https://www.mdpi.com/2075-4426/13/6/914 |
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