Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells
Several ecological studies suggest that ambient air pollution is associated with the occurrence of thyroid cancer. In this study, we used certified diesel particulate matter as a proxy for fine particulate matter. Human thyroid cancer cell lines 8505C and TPC-1 were incubated with different concentr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Toxicology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ftox.2023.1294760/full |
| _version_ | 1827630020739203072 |
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| author | Sheena Yi-Hsin Cheng Shih-Yuan Huang Shih-Ping Cheng Shih-Ping Cheng Shih-Ping Cheng |
| author_facet | Sheena Yi-Hsin Cheng Shih-Yuan Huang Shih-Ping Cheng Shih-Ping Cheng Shih-Ping Cheng |
| author_sort | Sheena Yi-Hsin Cheng |
| collection | DOAJ |
| description | Several ecological studies suggest that ambient air pollution is associated with the occurrence of thyroid cancer. In this study, we used certified diesel particulate matter as a proxy for fine particulate matter. Human thyroid cancer cell lines 8505C and TPC-1 were incubated with different concentrations of NIST1650b for 5 days and subjected to functional assays. We found that NIST1650b treatment did not affect short-term cell growth but reduced colony formation at high concentrations. Notably, NIST1650b-treated cells showed altered morphology toward cluster coalescence following treatment. Wound healing assays revealed that leading-edge cells formed protruding tips while maintaining cell-cell adhesion, and a significantly higher ratio of wound closure following treatment at 10 μg/mL was seen in both cell lines. A weak stimulatory effect on transwell cell migration was observed in 8505C cells. Taken together, our results suggest that fine particulate matter induced a coherent phenotype accompanied by augmented collective cell migration in thyroid cancer cells. |
| first_indexed | 2024-03-09T14:01:29Z |
| format | Article |
| id | doaj.art-f3adef7c1fcb43af8c4e1d20cbe47ac4 |
| institution | Directory Open Access Journal |
| issn | 2673-3080 |
| language | English |
| last_indexed | 2024-03-09T14:01:29Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Toxicology |
| spelling | doaj.art-f3adef7c1fcb43af8c4e1d20cbe47ac42023-11-30T08:16:19ZengFrontiers Media S.A.Frontiers in Toxicology2673-30802023-11-01510.3389/ftox.2023.12947601294760Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cellsSheena Yi-Hsin Cheng0Shih-Yuan Huang1Shih-Ping Cheng2Shih-Ping Cheng3Shih-Ping Cheng4Taipei European School, Taipei, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, Taipei, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, Taipei, TaiwanDepartment of Surgery, MacKay Memorial Hospital and MacKay Medical College, Taipei, TaiwanInstitute of Biomedical Sciences, MacKay Medical College, Taipei, TaiwanSeveral ecological studies suggest that ambient air pollution is associated with the occurrence of thyroid cancer. In this study, we used certified diesel particulate matter as a proxy for fine particulate matter. Human thyroid cancer cell lines 8505C and TPC-1 were incubated with different concentrations of NIST1650b for 5 days and subjected to functional assays. We found that NIST1650b treatment did not affect short-term cell growth but reduced colony formation at high concentrations. Notably, NIST1650b-treated cells showed altered morphology toward cluster coalescence following treatment. Wound healing assays revealed that leading-edge cells formed protruding tips while maintaining cell-cell adhesion, and a significantly higher ratio of wound closure following treatment at 10 μg/mL was seen in both cell lines. A weak stimulatory effect on transwell cell migration was observed in 8505C cells. Taken together, our results suggest that fine particulate matter induced a coherent phenotype accompanied by augmented collective cell migration in thyroid cancer cells.https://www.frontiersin.org/articles/10.3389/ftox.2023.1294760/fullfine particulate mattercollective cell migrationWound Healing Assayair pollutionthyroid cancer |
| spellingShingle | Sheena Yi-Hsin Cheng Shih-Yuan Huang Shih-Ping Cheng Shih-Ping Cheng Shih-Ping Cheng Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells Frontiers in Toxicology fine particulate matter collective cell migration Wound Healing Assay air pollution thyroid cancer |
| title | Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells |
| title_full | Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells |
| title_fullStr | Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells |
| title_full_unstemmed | Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells |
| title_short | Acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells |
| title_sort | acute exposure to diesel particulate matter promotes collective cell migration in thyroid cancer cells |
| topic | fine particulate matter collective cell migration Wound Healing Assay air pollution thyroid cancer |
| url | https://www.frontiersin.org/articles/10.3389/ftox.2023.1294760/full |
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