PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE
Aim. The study of immunochemical and immunobiological properties of native protein-containing antigens of pneumococcus. Materials and methods. The study was carried out on the strains of the Collective Usage Center «Collection of Mechnikov Res. Inst. for Vaccine and Sera». In the work studied the ch...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2019-08-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.elpub.ru/jour/article/view/352 |
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| author | D. S. Vorobyev I. B. Semenova Yu. V. Volokh E. E. Romanenko A. P. Baturo N. A. Mikhailova |
| author_facet | D. S. Vorobyev I. B. Semenova Yu. V. Volokh E. E. Romanenko A. P. Baturo N. A. Mikhailova |
| author_sort | D. S. Vorobyev |
| collection | DOAJ |
| description | Aim. The study of immunochemical and immunobiological properties of native protein-containing antigens of pneumococcus. Materials and methods. The study was carried out on the strains of the Collective Usage Center «Collection of Mechnikov Res. Inst. for Vaccine and Sera». In the work studied the chemical composition, the molecular weight of the obtained antigens in SDS-electrophoresis and antibody titers in ELISA. Protective activity of protein-containing antigens of pneumococcus was determined in experiments of active protection of mice. Results. Protein-containing antigens of pneumococcus were isolated from S. pneumoniae serotypes 3, 6B, 10A, 14, 19F, 23F and 36. The chemical composition of the preparations contained from 16 to 35% protein. In SDS-electrophoresis in polyacrylamide gel it was established that the molecular weight of protein-containing antigens of pneumococcus ranged from 14 to 116 kDa. Using ELISA shows the cross-activity of native antigens. Virtually all drugs reacted with antimicrobial rabbit serum obtained to serotype 19F (p≤0.05). Serotype serum 14 was less active and only protein-containing pneumococcal antigens obtained from 14 and 19F serotypes (p≤0.05) interacted with it. In the precipitation test according to Ouchterlony it was confirmed that preparations of serotypes 3, 6B, 14, 19F and 36 reacted with rabbit immune serum obtained for S. pneumoniae 19F serotype. In immunoblotting it was found that protein-containing antigens of pneumococcus isolated from serotypes 3, 6B, 10A, 14, 19F and 36 were associated with monoclonal antibodies to pneumococcal protein — pneumolysin. In vivo experiments it was shown that protein-containing antigens of pneumococcus protected animals from intraperitoneal infection of S. pneumoniae in homologous and heterologous systems (p≤0.05). Conclusion. The revealed immunochemical and cross-protective activity of protein-containing antigens of pneumococcus in vitro and in vivo experiments allows to select drugs derived from serotypes 6B, 10A, 19F and 36, as the most promising for further study of the intraspecific protective activity of individual native proteins of pneumococcus. |
| first_indexed | 2024-12-10T19:01:10Z |
| format | Article |
| id | doaj.art-f3b0220d4c9140679a9dc739e4415740 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:01:10Z |
| publishDate | 2019-08-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-f3b0220d4c9140679a9dc739e44157402022-12-22T01:37:00ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132019-08-0111222810.36233/0372-9311-2019-1-22-28344PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAED. S. Vorobyev0I. B. Semenova1Yu. V. Volokh2E. E. Romanenko3A. P. Baturo4N. A. Mikhailova5НИИ вакцин и сывороток им. И.И. МечниковаНИИ вакцин и сывороток им. И.И. МечниковаНИИ вакцин и сывороток им. И.И. МечниковаНИИ вакцин и сывороток им. И.И. МечниковаНИИ вакцин и сывороток им. И.И. МечниковаНИИ вакцин и сывороток им. И.И. МечниковаAim. The study of immunochemical and immunobiological properties of native protein-containing antigens of pneumococcus. Materials and methods. The study was carried out on the strains of the Collective Usage Center «Collection of Mechnikov Res. Inst. for Vaccine and Sera». In the work studied the chemical composition, the molecular weight of the obtained antigens in SDS-electrophoresis and antibody titers in ELISA. Protective activity of protein-containing antigens of pneumococcus was determined in experiments of active protection of mice. Results. Protein-containing antigens of pneumococcus were isolated from S. pneumoniae serotypes 3, 6B, 10A, 14, 19F, 23F and 36. The chemical composition of the preparations contained from 16 to 35% protein. In SDS-electrophoresis in polyacrylamide gel it was established that the molecular weight of protein-containing antigens of pneumococcus ranged from 14 to 116 kDa. Using ELISA shows the cross-activity of native antigens. Virtually all drugs reacted with antimicrobial rabbit serum obtained to serotype 19F (p≤0.05). Serotype serum 14 was less active and only protein-containing pneumococcal antigens obtained from 14 and 19F serotypes (p≤0.05) interacted with it. In the precipitation test according to Ouchterlony it was confirmed that preparations of serotypes 3, 6B, 14, 19F and 36 reacted with rabbit immune serum obtained for S. pneumoniae 19F serotype. In immunoblotting it was found that protein-containing antigens of pneumococcus isolated from serotypes 3, 6B, 10A, 14, 19F and 36 were associated with monoclonal antibodies to pneumococcal protein — pneumolysin. In vivo experiments it was shown that protein-containing antigens of pneumococcus protected animals from intraperitoneal infection of S. pneumoniae in homologous and heterologous systems (p≤0.05). Conclusion. The revealed immunochemical and cross-protective activity of protein-containing antigens of pneumococcus in vitro and in vivo experiments allows to select drugs derived from serotypes 6B, 10A, 19F and 36, as the most promising for further study of the intraspecific protective activity of individual native proteins of pneumococcus.https://microbiol.elpub.ru/jour/article/view/352pneumococcusprotein-containing antigens of pneumococcusserotype-independent vaccineimmunogenic activitycross-activityhomologous protectionheterologous protection |
| spellingShingle | D. S. Vorobyev I. B. Semenova Yu. V. Volokh E. E. Romanenko A. P. Baturo N. A. Mikhailova PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE Журнал микробиологии, эпидемиологии и иммунобиологии pneumococcus protein-containing antigens of pneumococcus serotype-independent vaccine immunogenic activity cross-activity homologous protection heterologous protection |
| title | PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE |
| title_full | PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE |
| title_fullStr | PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE |
| title_full_unstemmed | PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE |
| title_short | PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE |
| title_sort | properties of native protein containing antigens of streptococcus pneumoniae |
| topic | pneumococcus protein-containing antigens of pneumococcus serotype-independent vaccine immunogenic activity cross-activity homologous protection heterologous protection |
| url | https://microbiol.elpub.ru/jour/article/view/352 |
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