Temporal Observation of Adipocyte Microfiber Using Anchoring Device
In this paper, we propose an anchoring device with pillars to immobilize an adipocyte microfiber that has a fiber-shaped adipocyte tissue covered by an alginate gel shell. Because the device enabled the immobilization of the microfiber in a culture dish even after its transportation and the exchange...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/10/6/358 |
| _version_ | 1828749853196288000 |
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| author | Akiyo Yokomizo Yuya Morimoto Keigo Nishimura Shoji Takeuchi |
| author_facet | Akiyo Yokomizo Yuya Morimoto Keigo Nishimura Shoji Takeuchi |
| author_sort | Akiyo Yokomizo |
| collection | DOAJ |
| description | In this paper, we propose an anchoring device with pillars to immobilize an adipocyte microfiber that has a fiber-shaped adipocyte tissue covered by an alginate gel shell. Because the device enabled the immobilization of the microfiber in a culture dish even after its transportation and the exchange of the culture medium, we can easily track the specific positions of the microfiber for a long period. Owing to the characteristics of the anchoring device, we successfully performed temporal observations of the microfiber on the device for a month to investigate the function and morphology of three-dimensional cultured adipocytes. Furthermore, to demonstrate the applicability of the anchoring device to drug testing, we evaluated the lipolysis of the microfiber’s adipocytes by applying reagents with an anti-obesity effect. Therefore, we believe that the anchoring device with the microfiber will be a useful tool for temporal biochemical analyses. |
| first_indexed | 2024-12-10T20:29:24Z |
| format | Article |
| id | doaj.art-f3b134c6b6d34a01b0694b83f22dc6a0 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-10T20:29:24Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-f3b134c6b6d34a01b0694b83f22dc6a02022-12-22T01:34:45ZengMDPI AGMicromachines2072-666X2019-05-0110635810.3390/mi10060358mi10060358Temporal Observation of Adipocyte Microfiber Using Anchoring DeviceAkiyo Yokomizo0Yuya Morimoto1Keigo Nishimura2Shoji Takeuchi3Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, JapanCenter for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, JapanCenter for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, JapanCenter for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, JapanIn this paper, we propose an anchoring device with pillars to immobilize an adipocyte microfiber that has a fiber-shaped adipocyte tissue covered by an alginate gel shell. Because the device enabled the immobilization of the microfiber in a culture dish even after its transportation and the exchange of the culture medium, we can easily track the specific positions of the microfiber for a long period. Owing to the characteristics of the anchoring device, we successfully performed temporal observations of the microfiber on the device for a month to investigate the function and morphology of three-dimensional cultured adipocytes. Furthermore, to demonstrate the applicability of the anchoring device to drug testing, we evaluated the lipolysis of the microfiber’s adipocytes by applying reagents with an anti-obesity effect. Therefore, we believe that the anchoring device with the microfiber will be a useful tool for temporal biochemical analyses.https://www.mdpi.com/2072-666X/10/6/358microfluidicsbiofabricationadipose tissuelipolysis |
| spellingShingle | Akiyo Yokomizo Yuya Morimoto Keigo Nishimura Shoji Takeuchi Temporal Observation of Adipocyte Microfiber Using Anchoring Device Micromachines microfluidics biofabrication adipose tissue lipolysis |
| title | Temporal Observation of Adipocyte Microfiber Using Anchoring Device |
| title_full | Temporal Observation of Adipocyte Microfiber Using Anchoring Device |
| title_fullStr | Temporal Observation of Adipocyte Microfiber Using Anchoring Device |
| title_full_unstemmed | Temporal Observation of Adipocyte Microfiber Using Anchoring Device |
| title_short | Temporal Observation of Adipocyte Microfiber Using Anchoring Device |
| title_sort | temporal observation of adipocyte microfiber using anchoring device |
| topic | microfluidics biofabrication adipose tissue lipolysis |
| url | https://www.mdpi.com/2072-666X/10/6/358 |
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