Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp
Stimulator of interferon genes (STING) is crucial for the innate immune to defend against pathogenic infections. Our previous study showed that a STING homolog from Litopenaeus vannamei (LvSTING) was involved in antibacterial response via regulating antimicrobial peptides (AMPs). Nevertheless, how L...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.977327/full |
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| author | Haoyang Li Haoyang Li Haoyang Li Haoyang Li Haoyang Li Qinyao Li Qinyao Li Qinyao Li Qinyao Li Qinyao Li Sheng Wang Sheng Wang Sheng Wang Sheng Wang Sheng Wang Jianguo He Jianguo He Jianguo He Jianguo He Jianguo He Chaozheng Li Chaozheng Li Chaozheng Li Chaozheng Li Chaozheng Li |
| author_facet | Haoyang Li Haoyang Li Haoyang Li Haoyang Li Haoyang Li Qinyao Li Qinyao Li Qinyao Li Qinyao Li Qinyao Li Sheng Wang Sheng Wang Sheng Wang Sheng Wang Sheng Wang Jianguo He Jianguo He Jianguo He Jianguo He Jianguo He Chaozheng Li Chaozheng Li Chaozheng Li Chaozheng Li Chaozheng Li |
| author_sort | Haoyang Li |
| collection | DOAJ |
| description | Stimulator of interferon genes (STING) is crucial for the innate immune to defend against pathogenic infections. Our previous study showed that a STING homolog from Litopenaeus vannamei (LvSTING) was involved in antibacterial response via regulating antimicrobial peptides (AMPs). Nevertheless, how LvSTING induces AMPs expression to inhibit bacterial infection remains unknown. Herein, we revealed that the existence of a STING–IKKβ–Relish–AMPs axis in shrimp that was essential for opposing to Vibrio parahaemolyticus invasion. We observed that LvRelish was essential for host defense against V. parahaemolyticus infection via inducing several AMPs, such as LvALF1, LvCRU1, LvLYZ1 and LvPEN4. Knockdown of LvSTING or LvIKKβ in vivo led to the attenuated phosphorylation and diminished nuclear translocation of LvRelish, as well as the impaired expression levels of LvRelish-regulated AMPs. Accordingly, shrimps with knockdown of LvSTING or LvIKKβ or both were vulnerable to V. parahaemolyticus infection. Finally, LvSTING could recruit LvRelish and LvIKKβ to form a complex, which synergistically induced the promoter activity of several AMPs in vitro. Taken together, our results demonstrated that the shrimp STING–IKKβ–Relish–AMPs axis played a critical role in the defense against bacterial infection, and provided some insights into the development of disease prevention strategies in shrimp culture. |
| first_indexed | 2024-12-10T15:50:17Z |
| format | Article |
| id | doaj.art-f3b22c61378b4056b2c6fed2683e20be |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T15:50:17Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-f3b22c61378b4056b2c6fed2683e20be2022-12-22T01:42:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-08-011310.3389/fimmu.2022.977327977327Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimpHaoyang Li0Haoyang Li1Haoyang Li2Haoyang Li3Haoyang Li4Qinyao Li5Qinyao Li6Qinyao Li7Qinyao Li8Qinyao Li9Sheng Wang10Sheng Wang11Sheng Wang12Sheng Wang13Sheng Wang14Jianguo He15Jianguo He16Jianguo He17Jianguo He18Jianguo He19Chaozheng Li20Chaozheng Li21Chaozheng Li22Chaozheng Li23Chaozheng Li24State Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, ChinaGuangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaChina-ASEAN Belt and Road Joint Laboratory on Marine Aquaculture Technology, Zhuhai, ChinaState Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, ChinaGuangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaChina-ASEAN Belt and Road Joint Laboratory on Marine Aquaculture Technology, Zhuhai, ChinaState Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, ChinaGuangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaChina-ASEAN Belt and Road Joint Laboratory on Marine Aquaculture Technology, Zhuhai, ChinaState Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, ChinaGuangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaChina-ASEAN Belt and Road Joint Laboratory on Marine Aquaculture Technology, Zhuhai, ChinaState Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, ChinaGuangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaChina-ASEAN Belt and Road Joint Laboratory on Marine Aquaculture Technology, Zhuhai, ChinaStimulator of interferon genes (STING) is crucial for the innate immune to defend against pathogenic infections. Our previous study showed that a STING homolog from Litopenaeus vannamei (LvSTING) was involved in antibacterial response via regulating antimicrobial peptides (AMPs). Nevertheless, how LvSTING induces AMPs expression to inhibit bacterial infection remains unknown. Herein, we revealed that the existence of a STING–IKKβ–Relish–AMPs axis in shrimp that was essential for opposing to Vibrio parahaemolyticus invasion. We observed that LvRelish was essential for host defense against V. parahaemolyticus infection via inducing several AMPs, such as LvALF1, LvCRU1, LvLYZ1 and LvPEN4. Knockdown of LvSTING or LvIKKβ in vivo led to the attenuated phosphorylation and diminished nuclear translocation of LvRelish, as well as the impaired expression levels of LvRelish-regulated AMPs. Accordingly, shrimps with knockdown of LvSTING or LvIKKβ or both were vulnerable to V. parahaemolyticus infection. Finally, LvSTING could recruit LvRelish and LvIKKβ to form a complex, which synergistically induced the promoter activity of several AMPs in vitro. Taken together, our results demonstrated that the shrimp STING–IKKβ–Relish–AMPs axis played a critical role in the defense against bacterial infection, and provided some insights into the development of disease prevention strategies in shrimp culture.https://www.frontiersin.org/articles/10.3389/fimmu.2022.977327/fullLitopenaeus vannameiSTINGIKKβrelishvibrio parahaemolyticus |
| spellingShingle | Haoyang Li Haoyang Li Haoyang Li Haoyang Li Haoyang Li Qinyao Li Qinyao Li Qinyao Li Qinyao Li Qinyao Li Sheng Wang Sheng Wang Sheng Wang Sheng Wang Sheng Wang Jianguo He Jianguo He Jianguo He Jianguo He Jianguo He Chaozheng Li Chaozheng Li Chaozheng Li Chaozheng Li Chaozheng Li Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp Frontiers in Immunology Litopenaeus vannamei STING IKKβ relish vibrio parahaemolyticus |
| title | Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp |
| title_full | Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp |
| title_fullStr | Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp |
| title_full_unstemmed | Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp |
| title_short | Stimulator of interferon genes defends against bacterial infection via IKKβ-mediated Relish activation in shrimp |
| title_sort | stimulator of interferon genes defends against bacterial infection via ikkβ mediated relish activation in shrimp |
| topic | Litopenaeus vannamei STING IKKβ relish vibrio parahaemolyticus |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.977327/full |
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