Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens
The current study aimed to investigate the effect of different dietary supplemental oils on the immune status of broilers. One-day-old Cobb 500 broiler chicks were randomly distributed into eight batteries and fed eight experimental diets. There were 680 broilers, 85 birds per battery. The experimen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.01735/full |
| _version_ | 1818301184626130944 |
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| author | Hanan Al-Khalaifah Afaf Al-Nasser |
| author_facet | Hanan Al-Khalaifah Afaf Al-Nasser |
| author_sort | Hanan Al-Khalaifah |
| collection | DOAJ |
| description | The current study aimed to investigate the effect of different dietary supplemental oils on the immune status of broilers. One-day-old Cobb 500 broiler chicks were randomly distributed into eight batteries and fed eight experimental diets. There were 680 broilers, 85 birds per battery. The experimental oils were all used at 10% of the total diet. Each dietary treatment (TRT) contained one of the following essential oils: TRT 1 = control group that received a basal diet + soybean oil (SO); TRT 2 = basal diet as in TRT 1 + sunflower oil (SFO); TRT 3 = basal diet as in TRT 1 + canola oil (CO); TRT 4 = basal diet as in TRT 1 + flaxseed oil (FLO); TRT 5 = basal diet as in TRT 1 + fish oil (FO); TRT 6 = basal diet as in TRT 1 + mix of fish oil and soya oil (SO + FO); TRT 7 = basal diet as in TRT 1 + algal biomass oil (DHA); TRT 8 = basal diet as in TRT 1 + echium oil (EO). All samples were taken from 10 birds per treatment (n = 10). The immune parameters investigated involved measurement of weights of immune organs as a general indicator, hemocytometric measurements, intestinal microbial count and hindgut acidosis, hindgut volatile fatty acids, and cellular immune response using phytohemagglutinin test. The use of the different dietary treatments did not affect the general health status of the chickens, and the mortality was minimal with no signs of illness or outbreaks. The fact that both the control and the treatment diets were equally consumed would indicate that supplemental oil inclusions did not adversely affect the palatability of the diet by the chickens. At 3 weeks of age, there was no significant effect observed in the microbial counts of the intestine. However, at 5 weeks of age, the highest microbial count was significantly observed for broilers fed EO (7.30%), closely followed by SFO (6.95%), and the least microbial counts were observed for CO (5.63%). No significance was observed for lactic acid bacteria (LAB) and Salmonella. There was no significance observed for the effect of the dietary treatments on the hindgut volatile acid in the broilers. Wattle swelling changes were significant between dietary treatments. The results revealed that dietary FLO, FO, and DHA oils induced higher cellular response than the other treatments (P = 0.035), representing higher cellular response in these groups. In conclusion, supplemental oils rich in n−3 fatty acids may enhance the immune response in broiler chickens, represented by the intestinal microbial counts and the cellular immune response. |
| first_indexed | 2024-12-13T05:18:59Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-13T05:18:59Z |
| publishDate | 2020-08-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-f3b5c706b71341059ead8decc018c51d2022-12-21T23:58:22ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-08-011110.3389/fimmu.2020.01735551443Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler ChickensHanan Al-KhalaifahAfaf Al-NasserThe current study aimed to investigate the effect of different dietary supplemental oils on the immune status of broilers. One-day-old Cobb 500 broiler chicks were randomly distributed into eight batteries and fed eight experimental diets. There were 680 broilers, 85 birds per battery. The experimental oils were all used at 10% of the total diet. Each dietary treatment (TRT) contained one of the following essential oils: TRT 1 = control group that received a basal diet + soybean oil (SO); TRT 2 = basal diet as in TRT 1 + sunflower oil (SFO); TRT 3 = basal diet as in TRT 1 + canola oil (CO); TRT 4 = basal diet as in TRT 1 + flaxseed oil (FLO); TRT 5 = basal diet as in TRT 1 + fish oil (FO); TRT 6 = basal diet as in TRT 1 + mix of fish oil and soya oil (SO + FO); TRT 7 = basal diet as in TRT 1 + algal biomass oil (DHA); TRT 8 = basal diet as in TRT 1 + echium oil (EO). All samples were taken from 10 birds per treatment (n = 10). The immune parameters investigated involved measurement of weights of immune organs as a general indicator, hemocytometric measurements, intestinal microbial count and hindgut acidosis, hindgut volatile fatty acids, and cellular immune response using phytohemagglutinin test. The use of the different dietary treatments did not affect the general health status of the chickens, and the mortality was minimal with no signs of illness or outbreaks. The fact that both the control and the treatment diets were equally consumed would indicate that supplemental oil inclusions did not adversely affect the palatability of the diet by the chickens. At 3 weeks of age, there was no significant effect observed in the microbial counts of the intestine. However, at 5 weeks of age, the highest microbial count was significantly observed for broilers fed EO (7.30%), closely followed by SFO (6.95%), and the least microbial counts were observed for CO (5.63%). No significance was observed for lactic acid bacteria (LAB) and Salmonella. There was no significance observed for the effect of the dietary treatments on the hindgut volatile acid in the broilers. Wattle swelling changes were significant between dietary treatments. The results revealed that dietary FLO, FO, and DHA oils induced higher cellular response than the other treatments (P = 0.035), representing higher cellular response in these groups. In conclusion, supplemental oils rich in n−3 fatty acids may enhance the immune response in broiler chickens, represented by the intestinal microbial counts and the cellular immune response.https://www.frontiersin.org/article/10.3389/fimmu.2020.01735/fullbroilersessential oilsimmune responsehealthintestinemicrobial counts |
| spellingShingle | Hanan Al-Khalaifah Afaf Al-Nasser Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens Frontiers in Immunology broilers essential oils immune response health intestine microbial counts |
| title | Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens |
| title_full | Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens |
| title_fullStr | Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens |
| title_full_unstemmed | Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens |
| title_short | Dietary Supplementation With Various Fat Oils Affect Phytohemagglutinin Skin Test in Broiler Chickens |
| title_sort | dietary supplementation with various fat oils affect phytohemagglutinin skin test in broiler chickens |
| topic | broilers essential oils immune response health intestine microbial counts |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.01735/full |
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