The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration
Recent advances in biomaterials offer new possibilities for brain tissue reconstruction. Biocompatibility, provision of cell adhesion motives and mechanical properties are among the present main design criteria. We here propose a radically new and potentially major element determining biointegration...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/12/4/417 |
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| author | Fabien Bonini Sébastien Mosser Flavio Maurizio Mor Anissa Boutabla Patrick Burch Amélie Béduer Adrien Roux Thomas Braschler |
| author_facet | Fabien Bonini Sébastien Mosser Flavio Maurizio Mor Anissa Boutabla Patrick Burch Amélie Béduer Adrien Roux Thomas Braschler |
| author_sort | Fabien Bonini |
| collection | DOAJ |
| description | Recent advances in biomaterials offer new possibilities for brain tissue reconstruction. Biocompatibility, provision of cell adhesion motives and mechanical properties are among the present main design criteria. We here propose a radically new and potentially major element determining biointegration of porous biomaterials: the favorable effect of interstitial fluid pressure (IFP). The force applied by the lymphatic system through the interstitial fluid pressure on biomaterial integration has mostly been neglected so far. We hypothesize it has the potential to force 3D biointegration of porous biomaterials. In this study, we develop a capillary hydrostatic device to apply controlled in vitro interstitial fluid pressure and study its effect during 3D tissue culture. We find that the IFP is a key player in porous biomaterial tissue integration, at physiological IFP levels, surpassing the known effect of cell adhesion motives. Spontaneous electrical activity indicates that the culture conditions are not harmful for the cells. Our work identifies interstitial fluid pressure at physiological negative values as a potential main driver for tissue integration into porous biomaterials. We anticipate that controlling the IFP level could narrow the gap between in vivo and in vitro and therefore decrease the need for animal screening in biomaterial design. |
| first_indexed | 2024-03-09T11:05:41Z |
| format | Article |
| id | doaj.art-f3b730a7572542dd9fadd9e0c90aaf8f |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-09T11:05:41Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-f3b730a7572542dd9fadd9e0c90aaf8f2023-12-01T00:58:46ZengMDPI AGBrain Sciences2076-34252022-03-0112441710.3390/brainsci12040417The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial IntegrationFabien Bonini0Sébastien Mosser1Flavio Maurizio Mor2Anissa Boutabla3Patrick Burch4Amélie Béduer5Adrien Roux6Thomas Braschler7Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, SwitzerlandNeurix SA, Avenue de la Roseraie 64, CH-1022 Geneva, SwitzerlandHaute École du Paysage, d’Ingénierie et d’Architecture de Genève, Haute École Spécialisée de Suisse Occidentale (HEPIA HES-SO), University of Applied Sciences and Arts Western Switzerland, CH-1202 Geneva, SwitzerlandDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, SwitzerlandVolumina-Medical SA, Route de la Corniche 5, CH-1066 Epalinges, SwitzerlandDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, SwitzerlandHaute École du Paysage, d’Ingénierie et d’Architecture de Genève, Haute École Spécialisée de Suisse Occidentale (HEPIA HES-SO), University of Applied Sciences and Arts Western Switzerland, CH-1202 Geneva, SwitzerlandDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, SwitzerlandRecent advances in biomaterials offer new possibilities for brain tissue reconstruction. Biocompatibility, provision of cell adhesion motives and mechanical properties are among the present main design criteria. We here propose a radically new and potentially major element determining biointegration of porous biomaterials: the favorable effect of interstitial fluid pressure (IFP). The force applied by the lymphatic system through the interstitial fluid pressure on biomaterial integration has mostly been neglected so far. We hypothesize it has the potential to force 3D biointegration of porous biomaterials. In this study, we develop a capillary hydrostatic device to apply controlled in vitro interstitial fluid pressure and study its effect during 3D tissue culture. We find that the IFP is a key player in porous biomaterial tissue integration, at physiological IFP levels, surpassing the known effect of cell adhesion motives. Spontaneous electrical activity indicates that the culture conditions are not harmful for the cells. Our work identifies interstitial fluid pressure at physiological negative values as a potential main driver for tissue integration into porous biomaterials. We anticipate that controlling the IFP level could narrow the gap between in vivo and in vitro and therefore decrease the need for animal screening in biomaterial design.https://www.mdpi.com/2076-3425/12/4/417glymphatic systeminterstitial fluidbiointegrationbiomaterial |
| spellingShingle | Fabien Bonini Sébastien Mosser Flavio Maurizio Mor Anissa Boutabla Patrick Burch Amélie Béduer Adrien Roux Thomas Braschler The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration Brain Sciences glymphatic system interstitial fluid biointegration biomaterial |
| title | The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration |
| title_full | The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration |
| title_fullStr | The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration |
| title_full_unstemmed | The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration |
| title_short | The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration |
| title_sort | role of interstitial fluid pressure in cerebral porous biomaterial integration |
| topic | glymphatic system interstitial fluid biointegration biomaterial |
| url | https://www.mdpi.com/2076-3425/12/4/417 |
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