HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study.
Persistent inflammation contributes to the development of cardiovascular disease (CVD) as an HIV-associated comorbidity. Innate immune cells such as monocytes are major drivers of inflammation in men and women with HIV. The study objectives are to examine the contribution of circulating non-classica...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0285926 |
| _version_ | 1797337195668832256 |
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| author | Juan Lin Erik Ehinger David B Hanna Qibin Qi Tao Wang Yanal Ghosheh Karin Mueller Kathryn Anastos Jason M Lazar Wendy J Mack Phyllis C Tien Joan W Berman Mardge H Cohen Igho Ofotokun Stephen Gange Chenglong Liu Sonya L Heath Russell P Tracy Howard N Hodis Alan L Landay Klaus Ley Robert C Kaplan |
| author_facet | Juan Lin Erik Ehinger David B Hanna Qibin Qi Tao Wang Yanal Ghosheh Karin Mueller Kathryn Anastos Jason M Lazar Wendy J Mack Phyllis C Tien Joan W Berman Mardge H Cohen Igho Ofotokun Stephen Gange Chenglong Liu Sonya L Heath Russell P Tracy Howard N Hodis Alan L Landay Klaus Ley Robert C Kaplan |
| author_sort | Juan Lin |
| collection | DOAJ |
| description | Persistent inflammation contributes to the development of cardiovascular disease (CVD) as an HIV-associated comorbidity. Innate immune cells such as monocytes are major drivers of inflammation in men and women with HIV. The study objectives are to examine the contribution of circulating non-classical monocytes (NCM, CD14dimCD16+) and intermediate monocytes (IM, CD14+CD16+) to the host response to long-term HIV infection and HIV-associated CVD. Women with and without chronic HIV infection (H) were studied. Subclinical CVD (C) was detected as plaques imaged by B-mode carotid artery ultrasound. The study included H-C-, H+C-, H-C+, and H+C+ participants (23 of each, matched on race/ethnicity, age and smoking status), selected from among enrollees in the Women's Interagency HIV Study. We assessed transcriptomic features associated with HIV or CVD alone or comorbid HIV/CVD comparing to healthy (H-C-) participants in IM and NCM isolated from peripheral blood mononuclear cells. IM gene expression was little affected by HIV alone or CVD alone. In IM, coexisting HIV and CVD produced a measurable gene transcription signature, which was abolished by lipid-lowering treatment. In NCM, versus non-HIV controls, women with HIV had altered gene expression, irrespective of whether or not they had comorbid CVD. The largest set of differentially expressed genes was found in NCM among women with both HIV and CVD. Genes upregulated in association with HIV included several potential targets of drug therapies, including LAG3 (CD223). In conclusion, circulating monocytes from patients with well controlled HIV infection demonstrate an extensive gene expression signature which may be consistent with the ability of these cells to serve as potential viral reservoirs. Gene transcriptional changes in HIV patients were further magnified in the presence of subclinical CVD. |
| first_indexed | 2024-03-08T09:06:57Z |
| format | Article |
| id | doaj.art-f3b755da3995493bb9bc5b3e08f13d9a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T09:06:57Z |
| publishDate | 2023-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-f3b755da3995493bb9bc5b3e08f13d9a2024-02-01T05:32:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01185e028592610.1371/journal.pone.0285926HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study.Juan LinErik EhingerDavid B HannaQibin QiTao WangYanal GhoshehKarin MuellerKathryn AnastosJason M LazarWendy J MackPhyllis C TienJoan W BermanMardge H CohenIgho OfotokunStephen GangeChenglong LiuSonya L HeathRussell P TracyHoward N HodisAlan L LandayKlaus LeyRobert C KaplanPersistent inflammation contributes to the development of cardiovascular disease (CVD) as an HIV-associated comorbidity. Innate immune cells such as monocytes are major drivers of inflammation in men and women with HIV. The study objectives are to examine the contribution of circulating non-classical monocytes (NCM, CD14dimCD16+) and intermediate monocytes (IM, CD14+CD16+) to the host response to long-term HIV infection and HIV-associated CVD. Women with and without chronic HIV infection (H) were studied. Subclinical CVD (C) was detected as plaques imaged by B-mode carotid artery ultrasound. The study included H-C-, H+C-, H-C+, and H+C+ participants (23 of each, matched on race/ethnicity, age and smoking status), selected from among enrollees in the Women's Interagency HIV Study. We assessed transcriptomic features associated with HIV or CVD alone or comorbid HIV/CVD comparing to healthy (H-C-) participants in IM and NCM isolated from peripheral blood mononuclear cells. IM gene expression was little affected by HIV alone or CVD alone. In IM, coexisting HIV and CVD produced a measurable gene transcription signature, which was abolished by lipid-lowering treatment. In NCM, versus non-HIV controls, women with HIV had altered gene expression, irrespective of whether or not they had comorbid CVD. The largest set of differentially expressed genes was found in NCM among women with both HIV and CVD. Genes upregulated in association with HIV included several potential targets of drug therapies, including LAG3 (CD223). In conclusion, circulating monocytes from patients with well controlled HIV infection demonstrate an extensive gene expression signature which may be consistent with the ability of these cells to serve as potential viral reservoirs. Gene transcriptional changes in HIV patients were further magnified in the presence of subclinical CVD.https://doi.org/10.1371/journal.pone.0285926 |
| spellingShingle | Juan Lin Erik Ehinger David B Hanna Qibin Qi Tao Wang Yanal Ghosheh Karin Mueller Kathryn Anastos Jason M Lazar Wendy J Mack Phyllis C Tien Joan W Berman Mardge H Cohen Igho Ofotokun Stephen Gange Chenglong Liu Sonya L Heath Russell P Tracy Howard N Hodis Alan L Landay Klaus Ley Robert C Kaplan HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study. PLoS ONE |
| title | HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study. |
| title_full | HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study. |
| title_fullStr | HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study. |
| title_full_unstemmed | HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study. |
| title_short | HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women's Interagency HIV study. |
| title_sort | hiv infection and cardiovascular disease have both shared and distinct monocyte gene expression features women s interagency hiv study |
| url | https://doi.org/10.1371/journal.pone.0285926 |
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