Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor
Microbioreactors (MBRs) with a volume below 1 mL are promising alternatives to established cultivation platforms such as shake flasks, lab-scale bioreactors and microtiter plates. Their main advantages are simple automatization and parallelization and the saving of expensive media components and tes...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/7/512 |
| _version_ | 1827597835217928192 |
|---|---|
| author | Kevin Viebrock Dominik Rabl Sven Meinen Paul Wunder Jan-Angelus Meyer Lasse Jannis Frey Detlev Rasch Andreas Dietzel Torsten Mayr Rainer Krull |
| author_facet | Kevin Viebrock Dominik Rabl Sven Meinen Paul Wunder Jan-Angelus Meyer Lasse Jannis Frey Detlev Rasch Andreas Dietzel Torsten Mayr Rainer Krull |
| author_sort | Kevin Viebrock |
| collection | DOAJ |
| description | Microbioreactors (MBRs) with a volume below 1 mL are promising alternatives to established cultivation platforms such as shake flasks, lab-scale bioreactors and microtiter plates. Their main advantages are simple automatization and parallelization and the saving of expensive media components and test substances. These advantages are particularly pronounced in small-scale MBRs with a volume below 10 µL. However, most described small-scale MBRs are lacking in process information from integrated sensors due to limited space and sensor technology. Therefore, a novel capillary-wave microbioreactor (cwMBR) with a volume of only 7 µL has the potential to close this gap, as it combines a small volume with integrated sensors for biomass, pH, dissolved oxygen (DO) and glucose concentration. In the cwMBR, pH and DO are measured by established luminescent optical sensors on the bottom of the cwMBR. The novel glucose sensor is based on a modified oxygen sensor, which measures the oxygen uptake of glucose oxidase (GOx) in the presence of glucose up to a concentration of 15 mM. Furthermore, absorbance measurement allows biomass determination. The optical sensors enabled the characterization of an <i>Escherichia coli</i> batch cultivation over 8 h in the cwMBR as proof of concept for further bioprocesses. Hence, the cwMBR with integrated optical sensors has the potential for a wide range of microscale bioprocesses, including cell-based assays, screening applications and process development. |
| first_indexed | 2024-03-09T03:38:56Z |
| format | Article |
| id | doaj.art-f3b8453ce4b64e14af0bb8d8683c8738 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T03:38:56Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f3b8453ce4b64e14af0bb8d8683c87382023-12-03T14:44:20ZengMDPI AGBiosensors2079-63742022-07-0112751210.3390/bios12070512Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave MicrobioreactorKevin Viebrock0Dominik Rabl1Sven Meinen2Paul Wunder3Jan-Angelus Meyer4Lasse Jannis Frey5Detlev Rasch6Andreas Dietzel7Torsten Mayr8Rainer Krull9Institute of Biochemical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyInstitute of Analytical Chemistry and Food Chemistry, Technische Universität Graz, 8010 Graz, AustriaCenter of Pharmaceutical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyInstitute of Biochemical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyInstitute of Biochemical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyInstitute of Biochemical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyInstitute of Biochemical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyCenter of Pharmaceutical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyInstitute of Analytical Chemistry and Food Chemistry, Technische Universität Graz, 8010 Graz, AustriaInstitute of Biochemical Engineering, Technische Universität Braunschweig, 38106 Braunschweig, GermanyMicrobioreactors (MBRs) with a volume below 1 mL are promising alternatives to established cultivation platforms such as shake flasks, lab-scale bioreactors and microtiter plates. Their main advantages are simple automatization and parallelization and the saving of expensive media components and test substances. These advantages are particularly pronounced in small-scale MBRs with a volume below 10 µL. However, most described small-scale MBRs are lacking in process information from integrated sensors due to limited space and sensor technology. Therefore, a novel capillary-wave microbioreactor (cwMBR) with a volume of only 7 µL has the potential to close this gap, as it combines a small volume with integrated sensors for biomass, pH, dissolved oxygen (DO) and glucose concentration. In the cwMBR, pH and DO are measured by established luminescent optical sensors on the bottom of the cwMBR. The novel glucose sensor is based on a modified oxygen sensor, which measures the oxygen uptake of glucose oxidase (GOx) in the presence of glucose up to a concentration of 15 mM. Furthermore, absorbance measurement allows biomass determination. The optical sensors enabled the characterization of an <i>Escherichia coli</i> batch cultivation over 8 h in the cwMBR as proof of concept for further bioprocesses. Hence, the cwMBR with integrated optical sensors has the potential for a wide range of microscale bioprocesses, including cell-based assays, screening applications and process development.https://www.mdpi.com/2079-6374/12/7/512microbioreactoroptical sensorcapillary wavesglucose sensordroplet cultivation |
| spellingShingle | Kevin Viebrock Dominik Rabl Sven Meinen Paul Wunder Jan-Angelus Meyer Lasse Jannis Frey Detlev Rasch Andreas Dietzel Torsten Mayr Rainer Krull Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor Biosensors microbioreactor optical sensor capillary waves glucose sensor droplet cultivation |
| title | Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor |
| title_full | Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor |
| title_fullStr | Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor |
| title_full_unstemmed | Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor |
| title_short | Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor |
| title_sort | microsensor in microbioreactors full bioprocess characterization in a novel capillary wave microbioreactor |
| topic | microbioreactor optical sensor capillary waves glucose sensor droplet cultivation |
| url | https://www.mdpi.com/2079-6374/12/7/512 |
| work_keys_str_mv | AT kevinviebrock microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT dominikrabl microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT svenmeinen microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT paulwunder microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT janangelusmeyer microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT lassejannisfrey microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT detlevrasch microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT andreasdietzel microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT torstenmayr microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor AT rainerkrull microsensorinmicrobioreactorsfullbioprocesscharacterizationinanovelcapillarywavemicrobioreactor |