Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma
Background: Increasing evidence suggests the involvement of cancer stem cells (CSCs) in both oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). Among the various CSC markers, aldehyde dehydrogenase (ALDH) 1, B cell-specific Moloney murine leukaemia virus integration site 1 (Bmi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-09-01
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| Series: | Cancer Control |
| Online Access: | https://doi.org/10.1177/1073274820904959 |
| _version_ | 1797772283683536896 |
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| author | Roopa S. Rao MDS Lizbeth Raju K MDS Dominic Augustine MDS Shankargouda Patil MDS, PhD, MFDS-RCPS, FICD, FPFA |
| author_facet | Roopa S. Rao MDS Lizbeth Raju K MDS Dominic Augustine MDS Shankargouda Patil MDS, PhD, MFDS-RCPS, FICD, FPFA |
| author_sort | Roopa S. Rao MDS |
| collection | DOAJ |
| description | Background: Increasing evidence suggests the involvement of cancer stem cells (CSCs) in both oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). Among the various CSC markers, aldehyde dehydrogenase (ALDH) 1, B cell-specific Moloney murine leukaemia virus integration site 1 (Bmi1), and octamer-binding protein 4 (OCT4) have been noted to increase in OSCC. The aim of the study was to analyze ALDH1, Bmi1, and OCT4 expression in OED and OSCC with clinicopathologic correlation and survival analysis. Methods: A total of 40 cases each of OED and OSCC were retrieved from departmental archives. Expression of ALDH1, Bmi1, and OCT4 was analyzed using immunohistochemistry and was correlated with clinicopathological parameters. A follow-up ranging from 6 to 52 months was considered for Kaplan-Meier survival analysis. The log-rank test was performed to analyze significant difference in survival rates. Results: The expression levels of ALDH1, Bmi1, and OCT4 increased significantly from OED through OSCC ( P < .05). The expression of ALDH1 and OCT4 showed a significant correlation with lymph node metastasis. Positive cases of ALDH1 showed a significantly reduced survival rate compared to cases showing negative expression. Kaplan-Meier survival analysis showed a significant reduction of survival rate ( P = .00) in patients showing a positive expression for all the 3 markers. Conclusion: ALDH1 and OCT4 could be used as individual prognostic markers for assessing prognosis. ALDH1, Bmi1, and OCT4 could be used as a collective panel of markers to enable surgeons in predicting the prognosis of patients and thereby carry out prompt follow-up for such cases. |
| first_indexed | 2024-03-12T21:49:44Z |
| format | Article |
| id | doaj.art-f3b866b122af40b79e00e4e0de50e8fe |
| institution | Directory Open Access Journal |
| issn | 1073-2748 |
| language | English |
| last_indexed | 2024-03-12T21:49:44Z |
| publishDate | 2020-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cancer Control |
| spelling | doaj.art-f3b866b122af40b79e00e4e0de50e8fe2023-07-26T06:36:11ZengSAGE PublishingCancer Control1073-27482020-09-012710.1177/1073274820904959Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell CarcinomaRoopa S. Rao MDS0Lizbeth Raju K MDS1Dominic Augustine MDS2Shankargouda Patil MDS, PhD, MFDS-RCPS, FICD, FPFA3 Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, , India Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, , India Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, , India Department of Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Jazan University, Saudi ArabiaBackground: Increasing evidence suggests the involvement of cancer stem cells (CSCs) in both oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). Among the various CSC markers, aldehyde dehydrogenase (ALDH) 1, B cell-specific Moloney murine leukaemia virus integration site 1 (Bmi1), and octamer-binding protein 4 (OCT4) have been noted to increase in OSCC. The aim of the study was to analyze ALDH1, Bmi1, and OCT4 expression in OED and OSCC with clinicopathologic correlation and survival analysis. Methods: A total of 40 cases each of OED and OSCC were retrieved from departmental archives. Expression of ALDH1, Bmi1, and OCT4 was analyzed using immunohistochemistry and was correlated with clinicopathological parameters. A follow-up ranging from 6 to 52 months was considered for Kaplan-Meier survival analysis. The log-rank test was performed to analyze significant difference in survival rates. Results: The expression levels of ALDH1, Bmi1, and OCT4 increased significantly from OED through OSCC ( P < .05). The expression of ALDH1 and OCT4 showed a significant correlation with lymph node metastasis. Positive cases of ALDH1 showed a significantly reduced survival rate compared to cases showing negative expression. Kaplan-Meier survival analysis showed a significant reduction of survival rate ( P = .00) in patients showing a positive expression for all the 3 markers. Conclusion: ALDH1 and OCT4 could be used as individual prognostic markers for assessing prognosis. ALDH1, Bmi1, and OCT4 could be used as a collective panel of markers to enable surgeons in predicting the prognosis of patients and thereby carry out prompt follow-up for such cases.https://doi.org/10.1177/1073274820904959 |
| spellingShingle | Roopa S. Rao MDS Lizbeth Raju K MDS Dominic Augustine MDS Shankargouda Patil MDS, PhD, MFDS-RCPS, FICD, FPFA Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma Cancer Control |
| title | Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma |
| title_full | Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma |
| title_fullStr | Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma |
| title_short | Prognostic Significance of ALDH1, Bmi1, and OCT4 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma |
| title_sort | prognostic significance of aldh1 bmi1 and oct4 expression in oral epithelial dysplasia and oral squamous cell carcinoma |
| url | https://doi.org/10.1177/1073274820904959 |
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