| Summary: | Interaction between the chemokine receptor <i>XCR1</i> and its ligand is closely related to the immune function in animals; however, there are only a few reports on role of <i>XCR1</i> in the immune system of fish. We aimed to analyze the expression of <i>XCR1</i> in various organs or tissues of grass carp before and after Grass Carp Reovirus (GCRV) infection to better understand the function of <i>XCR1</i> in resistance to GCRV infection. We cloned and sequenced the cDNA of grass carp <i>XCR1</i> and analyzed the molecular structure of <i>XCR1</i> based its amino acid sequence. Further, we analyzed the relative expression levels of <i>XCR1</i> in different organs or tissues of male parent grass carp with GCRV resistance (P1) and their first-generation offspring (F1) before and after GCRV infection. Our results show that the total length of cDNA of the grass carp <i>XCR1</i> gene is 1659 bp and encodes 365 amino acids. <i>XCR1</i> contains seven conserved transmembrane helical domains. The homologous tertiary structure of <i>XCR1</i> is similar to its homologs in other species. After artificial GCRV infection, there were significant differences in the expression of the grass carp <i>XCR1</i> gene in different tissues, at different time points, and between P1 and F1 fish. These results will contribute to our understanding of the role of <i>XCR1</i> in fish immune responses and contribute to the development of GCRV-resistant grass carp.
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