alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade
Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Despite progress in the last decades, there are still no reliable biomarkers for the diagnosis of and prognosis for GC. Aberrant sialylation is a widespread critical event in the development of GC. Neuraminidases (...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-09-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3330 |
| _version_ | 1811258143446925312 |
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| author | Michael W.L. Quirino Amanda P.B. Albuquerque Maria F.D. de Souza Antônio F. da Silva Filho Mário R. Martins Maira G. da Rocha Pitta Michelly C. Pereira Moacyr J.B. de Melo Rêgo |
| author_facet | Michael W.L. Quirino Amanda P.B. Albuquerque Maria F.D. de Souza Antônio F. da Silva Filho Mário R. Martins Maira G. da Rocha Pitta Michelly C. Pereira Moacyr J.B. de Melo Rêgo |
| author_sort | Michael W.L. Quirino |
| collection | DOAJ |
| description |
Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Despite progress in the last decades, there are still no reliable biomarkers for the diagnosis of and prognosis for GC. Aberrant sialylation is a widespread critical event in the development of GC. Neuraminidases (Neu) and sialyltransferases (STs) regulate the ablation and addition of sialic acid during glycoconjugates biosynthesis, and they are a considerable source of biomarkers in various cancers. This study retrospectively characterized Neu3 and ST3Gal3 expression by immunohistochemistry in 71 paraffin-embedded GC tissue specimens and analyzed the relationship between their expression and the clinicopathological parameters. Neu3 expression was markedly increased in GC tissues compared with non-tumoral tissues (p<0.0001). Intratumoral ST3Gal3 staining was significantly associated with intestinal subtype (p=0.0042) and was negatively associated with angiolymphatic invasion (p=0.0002) and higher histological grade G3 (p=0.0066). Multivariate analysis revealed that ST3Gal3 positivity is able to predict Lauren's classification. No associations were found between Neu3 staining and clinical parameters. The in silico analysis of mRNA expression in GC validation cohorts corroborates the significant ST3Gal3 association with higher histological grade observed in our study. These findings suggest that ST3Gal3 expression may be an indicator for aggressiveness of primary GC.
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| first_indexed | 2024-04-12T18:09:39Z |
| format | Article |
| id | doaj.art-f3bd1b26a13c415481e27f9e50e99774 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-12T18:09:39Z |
| publishDate | 2022-09-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-f3bd1b26a13c415481e27f9e50e997742022-12-22T03:21:53ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062022-09-0166410.4081/ejh.2022.3330alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological gradeMichael W.L. Quirino0Amanda P.B. Albuquerque1Maria F.D. de Souza2Antônio F. da Silva Filho3Mário R. Martins4Maira G. da Rocha Pitta5Michelly C. Pereira6Moacyr J.B. de Melo Rêgo7Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PELaboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PELaboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PELaboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PEPernambuco Cancer Hospital - HCP, Recife, PELaboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PELaboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PELaboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for -Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Despite progress in the last decades, there are still no reliable biomarkers for the diagnosis of and prognosis for GC. Aberrant sialylation is a widespread critical event in the development of GC. Neuraminidases (Neu) and sialyltransferases (STs) regulate the ablation and addition of sialic acid during glycoconjugates biosynthesis, and they are a considerable source of biomarkers in various cancers. This study retrospectively characterized Neu3 and ST3Gal3 expression by immunohistochemistry in 71 paraffin-embedded GC tissue specimens and analyzed the relationship between their expression and the clinicopathological parameters. Neu3 expression was markedly increased in GC tissues compared with non-tumoral tissues (p<0.0001). Intratumoral ST3Gal3 staining was significantly associated with intestinal subtype (p=0.0042) and was negatively associated with angiolymphatic invasion (p=0.0002) and higher histological grade G3 (p=0.0066). Multivariate analysis revealed that ST3Gal3 positivity is able to predict Lauren's classification. No associations were found between Neu3 staining and clinical parameters. The in silico analysis of mRNA expression in GC validation cohorts corroborates the significant ST3Gal3 association with higher histological grade observed in our study. These findings suggest that ST3Gal3 expression may be an indicator for aggressiveness of primary GC. https://www.ejh.it/index.php/ejh/article/view/3330Neuraminidase 3primary gastric cancerST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
| spellingShingle | Michael W.L. Quirino Amanda P.B. Albuquerque Maria F.D. de Souza Antônio F. da Silva Filho Mário R. Martins Maira G. da Rocha Pitta Michelly C. Pereira Moacyr J.B. de Melo Rêgo alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade European Journal of Histochemistry Neuraminidase 3 primary gastric cancer ST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
| title | alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade |
| title_full | alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade |
| title_fullStr | alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade |
| title_full_unstemmed | alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade |
| title_short | alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade |
| title_sort | alpha2 3 sialic acid processing enzymes expression in gastric cancer tissues reveals that st3gal3 but not neu3 are associated with lauren s classification angiolymphatic invasion and histological grade |
| topic | Neuraminidase 3 primary gastric cancer ST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
| url | https://www.ejh.it/index.php/ejh/article/view/3330 |
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