Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures – An Experimental Study
<p><strong>Background and Objective:</strong> Radiofrequency ablation (RFA) is a safe and less invasive technique that uses an electric current to damage nerve tissue to stop it from sending pain signals. This study was aimed to determine the molecular mechanism of neuropathologic...
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| Format: | Article |
| Language: | English |
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Discover STM Publishing Ltd
2021-12-01
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| Series: | BioMedica |
| Online Access: | https://biomedicapk.com/10.51441/BioMedica/5-519 |
| _version_ | 1797629381463506944 |
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| author | Li Su Yu Dong Ying Chen Baoguo Yao Peng Song Ruiting Xu Rui Li Ping Liu Yu Zhang Li Mu Xin Tong Linwei Ma Jianjun Yu |
| author_facet | Li Su Yu Dong Ying Chen Baoguo Yao Peng Song Ruiting Xu Rui Li Ping Liu Yu Zhang Li Mu Xin Tong Linwei Ma Jianjun Yu |
| author_sort | Li Su |
| collection | DOAJ |
| description | <p><strong>Background and Objective:</strong> Radiofrequency ablation (RFA) is a safe and less invasive technique that uses an electric current to damage nerve tissue to stop it from sending pain signals. This study was aimed to determine the molecular mechanism of neuropathologic damage induced by RFA at different temperatures.<br /><strong>Methods:</strong> A total of 36 Sprague Dawley rats were used as model with neuropathological injury. These rats were divided into six groups based on the temperature stimulation at 42°C, 47°C, 52°C, 57°C, 62°C, and 67°C. Conduction time, distance, and velocity were recorded after thermal injury. Hematoxylin and eosin staining was used to observe the histopathological changes of sciatic nerve. Neural ion channel proteins such as sodium voltage-gated channel alpha subunit 9 (SCN9A), sodium channel B3 subunit (SCN3B) and neurofascin (NFASC) expression in sciatic nerve tissue were detected detected by Western Blot.<br /><strong>Results:</strong> Nerve conduction velocity (NCV) gradually decreased with the increase in temperature and neuronal damage was seen at 67°C. H&E staining showed increased degeneration of neurons with an increase in temperature from 47°C to 67°C. SCN9A and SCN3B expression at 57°C, 62°C, and 67°C was much higher; however, NFASC expression was lower at same temperatures.<br /><strong>Conclusion:</strong> Neuropathological damage caused by RFA at different temperatures shows positive correlation with NCV. Heat transfer injury affects the expression of SCN9A, SCN3B, and NFASC in sciatic nerve tissue. </p> |
| first_indexed | 2024-03-11T10:53:40Z |
| format | Article |
| id | doaj.art-f3be8f1fa5b64407bef1d98f73f215b2 |
| institution | Directory Open Access Journal |
| issn | 2710-3471 |
| language | English |
| last_indexed | 2024-03-11T10:53:40Z |
| publishDate | 2021-12-01 |
| publisher | Discover STM Publishing Ltd |
| record_format | Article |
| series | BioMedica |
| spelling | doaj.art-f3be8f1fa5b64407bef1d98f73f215b22023-11-13T14:11:52ZengDiscover STM Publishing LtdBioMedica2710-34712021-12-0137423423910.51441/BioMedica/5-519Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental StudyLi Su0Yu Dong1Ying Chen2Baoguo Yao3Peng Song4Ruiting Xu5Rui Li6Ping Liu7Yu Zhang8Li Mu9Xin Tong10Linwei Ma11Jianjun Yu12Department of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, ChinaDepartment of Thyroid and Breast Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, China<p><strong>Background and Objective:</strong> Radiofrequency ablation (RFA) is a safe and less invasive technique that uses an electric current to damage nerve tissue to stop it from sending pain signals. This study was aimed to determine the molecular mechanism of neuropathologic damage induced by RFA at different temperatures.<br /><strong>Methods:</strong> A total of 36 Sprague Dawley rats were used as model with neuropathological injury. These rats were divided into six groups based on the temperature stimulation at 42°C, 47°C, 52°C, 57°C, 62°C, and 67°C. Conduction time, distance, and velocity were recorded after thermal injury. Hematoxylin and eosin staining was used to observe the histopathological changes of sciatic nerve. Neural ion channel proteins such as sodium voltage-gated channel alpha subunit 9 (SCN9A), sodium channel B3 subunit (SCN3B) and neurofascin (NFASC) expression in sciatic nerve tissue were detected detected by Western Blot.<br /><strong>Results:</strong> Nerve conduction velocity (NCV) gradually decreased with the increase in temperature and neuronal damage was seen at 67°C. H&E staining showed increased degeneration of neurons with an increase in temperature from 47°C to 67°C. SCN9A and SCN3B expression at 57°C, 62°C, and 67°C was much higher; however, NFASC expression was lower at same temperatures.<br /><strong>Conclusion:</strong> Neuropathological damage caused by RFA at different temperatures shows positive correlation with NCV. Heat transfer injury affects the expression of SCN9A, SCN3B, and NFASC in sciatic nerve tissue. </p>https://biomedicapk.com/10.51441/BioMedica/5-519 |
| spellingShingle | Li Su Yu Dong Ying Chen Baoguo Yao Peng Song Ruiting Xu Rui Li Ping Liu Yu Zhang Li Mu Xin Tong Linwei Ma Jianjun Yu Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental Study BioMedica |
| title | Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental Study |
| title_full | Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental Study |
| title_fullStr | Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental Study |
| title_full_unstemmed | Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental Study |
| title_short | Neuropathologic Damage Induced by Radiofrequency Ablation at Different Temperatures &ndash; An Experimental Study |
| title_sort | neuropathologic damage induced by radiofrequency ablation at different temperatures amp ndash an experimental study |
| url | https://biomedicapk.com/10.51441/BioMedica/5-519 |
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