Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain
In South Korea in 2013, the G1-based vaccine failed to prevent an outbreak of G2b-type porcine epidemic diarrhea virus (PEDV), which is more pathogenic than the traditional G1-type strain, thereby allowing the virus to spread. In 2017 and 2018, field samples were cultured sequentially on Vero cells...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/7/1601 |
| _version_ | 1797587270777176064 |
|---|---|
| author | Sok Song Gyu-Nam Park Jihye Shin Ki-Sun Kim Byung-Hyun An SeEun Choe Song-Yi Kim Bang-Hun Hyun Dong-Jun An |
| author_facet | Sok Song Gyu-Nam Park Jihye Shin Ki-Sun Kim Byung-Hyun An SeEun Choe Song-Yi Kim Bang-Hun Hyun Dong-Jun An |
| author_sort | Sok Song |
| collection | DOAJ |
| description | In South Korea in 2013, the G1-based vaccine failed to prevent an outbreak of G2b-type porcine epidemic diarrhea virus (PEDV), which is more pathogenic than the traditional G1-type strain, thereby allowing the virus to spread. In 2017 and 2018, field samples were cultured sequentially on Vero cells to isolate HS (virulent) and SGP-M1 (partially attenuated) strains, respectively, of the G2b type. The HS strain harbors a single amino acid (aa) change and two aa deletions in the N-terminal domain of S1 (<sup>55</sup>I<sup>56</sup>G<sup>57</sup>E→<sup>55</sup>K<sup>56</sup>Δ<sup>57</sup>Δ). The SGP-M1 strain harbors a seven aa deletion in the C-terminal domain of S2 (<sup>1380~1386</sup>ΔFEKVHVQ). By co-infecting various animal cells with these two strains (HS and SGP-M1), we succeeded in cloning strain HSGP, which harbors the mutations present in the two original viruses. The CPE pattern of the HSGP strain was different from that of the HS and SGP-M1 strains, with higher viral titers. Studies in piglets showed attenuated pathogenicity of the HSGP strain, with no clinical symptoms or viral shedding, and histopathologic lesions similar to those in negative controls. These findings confirm that deletion of specific sequences from the S gene attenuates the pathogenicity of PEDV. In addition, HSGP strains created by combining two different strains have the potential for use as novel attenuated live vaccine candidates. |
| first_indexed | 2024-03-11T00:33:46Z |
| format | Article |
| id | doaj.art-f3c029de5b6947e2866f6f74d86ed73a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T00:33:46Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f3c029de5b6947e2866f6f74d86ed73a2023-11-18T21:46:00ZengMDPI AGViruses1999-49152023-07-01157160110.3390/v15071601Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated StrainSok Song0Gyu-Nam Park1Jihye Shin2Ki-Sun Kim3Byung-Hyun An4SeEun Choe5Song-Yi Kim6Bang-Hun Hyun7Dong-Jun An8Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaCollege of Veterinary Medicine, Seoul University, Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaVirus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of KoreaIn South Korea in 2013, the G1-based vaccine failed to prevent an outbreak of G2b-type porcine epidemic diarrhea virus (PEDV), which is more pathogenic than the traditional G1-type strain, thereby allowing the virus to spread. In 2017 and 2018, field samples were cultured sequentially on Vero cells to isolate HS (virulent) and SGP-M1 (partially attenuated) strains, respectively, of the G2b type. The HS strain harbors a single amino acid (aa) change and two aa deletions in the N-terminal domain of S1 (<sup>55</sup>I<sup>56</sup>G<sup>57</sup>E→<sup>55</sup>K<sup>56</sup>Δ<sup>57</sup>Δ). The SGP-M1 strain harbors a seven aa deletion in the C-terminal domain of S2 (<sup>1380~1386</sup>ΔFEKVHVQ). By co-infecting various animal cells with these two strains (HS and SGP-M1), we succeeded in cloning strain HSGP, which harbors the mutations present in the two original viruses. The CPE pattern of the HSGP strain was different from that of the HS and SGP-M1 strains, with higher viral titers. Studies in piglets showed attenuated pathogenicity of the HSGP strain, with no clinical symptoms or viral shedding, and histopathologic lesions similar to those in negative controls. These findings confirm that deletion of specific sequences from the S gene attenuates the pathogenicity of PEDV. In addition, HSGP strains created by combining two different strains have the potential for use as novel attenuated live vaccine candidates.https://www.mdpi.com/1999-4915/15/7/1601PEDVmutationpigletdiarrheaspikedeletion |
| spellingShingle | Sok Song Gyu-Nam Park Jihye Shin Ki-Sun Kim Byung-Hyun An SeEun Choe Song-Yi Kim Bang-Hun Hyun Dong-Jun An Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain Viruses PEDV mutation piglet diarrhea spike deletion |
| title | Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain |
| title_full | Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain |
| title_fullStr | Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain |
| title_full_unstemmed | Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain |
| title_short | Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated Strain |
| title_sort | rescue of a live attenuated porcine epidemic diarrhea virus hsgp strain using a virulent strain and a partially attenuated strain |
| topic | PEDV mutation piglet diarrhea spike deletion |
| url | https://www.mdpi.com/1999-4915/15/7/1601 |
| work_keys_str_mv | AT soksong rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT gyunampark rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT jihyeshin rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT kisunkim rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT byunghyunan rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT seeunchoe rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT songyikim rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT banghunhyun rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain AT dongjunan rescueofaliveattenuatedporcineepidemicdiarrheavirushsgpstrainusingavirulentstrainandapartiallyattenuatedstrain |