Rat Model of Quadriceps Contracture by Joint Immobilization
Muscle contracture is an abnormal pathologic process resulting in fibrosis and muscle atrophy, which can lead to limitation of joint motion. To establish a diagnostic method to detect muscle contracture and a method to control its progression, we investigated an appropriate method to create an anima...
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MDPI AG
2022-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/12/1781 |
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| author | Kanokwan Suwankanit Miki Shimizu |
| author_facet | Kanokwan Suwankanit Miki Shimizu |
| author_sort | Kanokwan Suwankanit |
| collection | DOAJ |
| description | Muscle contracture is an abnormal pathologic process resulting in fibrosis and muscle atrophy, which can lead to limitation of joint motion. To establish a diagnostic method to detect muscle contracture and a method to control its progression, we investigated an appropriate method to create an animal model of quadriceps contracture using rats. Eighteen Wistar rats were divided into three groups, and bilateral hindlimbs were immobilized with either a cast (Group I), a Velcro hook-and-loop fastener (Group V), or steel wire (Group S) with the knee and ankle joints in extension position for two weeks. Five rats in a control group (Group C) were not immobilized. After two weeks, the progression of quadriceps contracture was assessed by measuring the range of joint motion and pathohistological changes. Muscle atrophy and fibrosis were observed in all immobilization groups. The knee joint range of motion, quadriceps muscle weight, and muscle fiber size decreased only in Group S compared to the other immobilization groups. Stress on rats due to immobilization was less in Group S. These results indicate that Group S is the superior quadriceps contracture model. This model aids research investigating diagnostic and therapeutic methods for muscle contracture in humans and animals. |
| first_indexed | 2024-03-09T17:19:42Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T17:19:42Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-f3c0f1c2949d4ec6a58bcc8bccc08b7e2023-11-24T13:23:22ZengMDPI AGBiology2079-77372022-12-011112178110.3390/biology11121781Rat Model of Quadriceps Contracture by Joint ImmobilizationKanokwan Suwankanit0Miki Shimizu1Department of Veterinary Diagnostic Imaging, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Tokyo 183-0054, JapanDepartment of Veterinary Diagnostic Imaging, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Tokyo 183-0054, JapanMuscle contracture is an abnormal pathologic process resulting in fibrosis and muscle atrophy, which can lead to limitation of joint motion. To establish a diagnostic method to detect muscle contracture and a method to control its progression, we investigated an appropriate method to create an animal model of quadriceps contracture using rats. Eighteen Wistar rats were divided into three groups, and bilateral hindlimbs were immobilized with either a cast (Group I), a Velcro hook-and-loop fastener (Group V), or steel wire (Group S) with the knee and ankle joints in extension position for two weeks. Five rats in a control group (Group C) were not immobilized. After two weeks, the progression of quadriceps contracture was assessed by measuring the range of joint motion and pathohistological changes. Muscle atrophy and fibrosis were observed in all immobilization groups. The knee joint range of motion, quadriceps muscle weight, and muscle fiber size decreased only in Group S compared to the other immobilization groups. Stress on rats due to immobilization was less in Group S. These results indicate that Group S is the superior quadriceps contracture model. This model aids research investigating diagnostic and therapeutic methods for muscle contracture in humans and animals.https://www.mdpi.com/2079-7737/11/12/1781joint range of motionmuscle weightmuscle lengthcross-sectional area of muscle fibercollagen percentagequadriceps contracture |
| spellingShingle | Kanokwan Suwankanit Miki Shimizu Rat Model of Quadriceps Contracture by Joint Immobilization Biology joint range of motion muscle weight muscle length cross-sectional area of muscle fiber collagen percentage quadriceps contracture |
| title | Rat Model of Quadriceps Contracture by Joint Immobilization |
| title_full | Rat Model of Quadriceps Contracture by Joint Immobilization |
| title_fullStr | Rat Model of Quadriceps Contracture by Joint Immobilization |
| title_full_unstemmed | Rat Model of Quadriceps Contracture by Joint Immobilization |
| title_short | Rat Model of Quadriceps Contracture by Joint Immobilization |
| title_sort | rat model of quadriceps contracture by joint immobilization |
| topic | joint range of motion muscle weight muscle length cross-sectional area of muscle fiber collagen percentage quadriceps contracture |
| url | https://www.mdpi.com/2079-7737/11/12/1781 |
| work_keys_str_mv | AT kanokwansuwankanit ratmodelofquadricepscontracturebyjointimmobilization AT mikishimizu ratmodelofquadricepscontracturebyjointimmobilization |