Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact
Objective Sequelae of behavioral impairments associated with human traumatic brain injury (TBI) include neurobehavioral problems. We compared exploratory, cognitive, and depressive-like behaviors in pediatric and adult male mice exposed to controlled cortical impact (CCI). Methods Pediatric (21 to 2...
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| Format: | Article |
| Language: | English |
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The Korean Society of Emergency Medicine
2019-06-01
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| Series: | Clinical and Experimental Emergency Medicine |
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| Online Access: | http://www.ceemjournal.org/upload/pdf/ceem-18-019.pdf |
| _version_ | 1797895658227630080 |
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| author | Suk-Woo Lee Mun-Sun Jang Seong-Hae Jeong Hoon Kim |
| author_facet | Suk-Woo Lee Mun-Sun Jang Seong-Hae Jeong Hoon Kim |
| author_sort | Suk-Woo Lee |
| collection | DOAJ |
| description | Objective Sequelae of behavioral impairments associated with human traumatic brain injury (TBI) include neurobehavioral problems. We compared exploratory, cognitive, and depressive-like behaviors in pediatric and adult male mice exposed to controlled cortical impact (CCI). Methods Pediatric (21 to 25 days old) and adult (8 to 12 weeks old) male C57Bl/6 mice underwent CCI at a 2-mm depth of deflection. Hematoxylin and eosin staining was performed 3 to 7 days after recovery from CCI, and injury volume was analyzed using ImageJ. Neurobehavioral characterization after CCI was performed using the Barnes maze test (BMT), passive avoidance test, open-field test, light/dark test, tail suspension test, and rotarod test. Acutely and subacutely (3 and 7 days after CCI, respectively), CCI mice showed graded injury compared to sham mice for all analyzed deflection depths. Results Time-dependent differences in injury volume were noted between 3 and 7 days following 2-mm TBI in adult mice. In the BMT, 2-mm TBI adults showed spatial memory deficits compared to sham adults (P<0.05). However, no difference in spatial learning and memory was found between sham and 2-mm CCI groups among pediatric mice. The open-field test, light/dark test, and tail suspension test did not reveal differences in anxiety-like behaviors in both age groups. Conclusion Our findings revealed a graded injury response in both age groups. The BMT was an efficient cognitive test for assessing spatial/non-spatial learning following CCI in adult mice; however, spatial learning impairments in pediatric mice could not be assessed. |
| first_indexed | 2024-04-10T07:29:19Z |
| format | Article |
| id | doaj.art-f3c1499240c849f388e432ad90974dab |
| institution | Directory Open Access Journal |
| issn | 2383-4625 |
| language | English |
| last_indexed | 2024-04-10T07:29:19Z |
| publishDate | 2019-06-01 |
| publisher | The Korean Society of Emergency Medicine |
| record_format | Article |
| series | Clinical and Experimental Emergency Medicine |
| spelling | doaj.art-f3c1499240c849f388e432ad90974dab2023-02-24T02:14:43ZengThe Korean Society of Emergency MedicineClinical and Experimental Emergency Medicine2383-46252019-06-016212513710.15441/ceem.18.019235Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impactSuk-Woo Lee0Mun-Sun Jang1Seong-Hae Jeong2Hoon Kim3 Department of Emergency Medicine, Chungbuk National University Hospital, Cheongju, Korea Department of Emergency Medicine, Chungbuk National University College of Medicine, Cheongju, Korea Department of Neurology, Chungnam National University College of Medicine, Daejeon, Korea Department of Emergency Medicine, Chungbuk National University Hospital, Cheongju, KoreaObjective Sequelae of behavioral impairments associated with human traumatic brain injury (TBI) include neurobehavioral problems. We compared exploratory, cognitive, and depressive-like behaviors in pediatric and adult male mice exposed to controlled cortical impact (CCI). Methods Pediatric (21 to 25 days old) and adult (8 to 12 weeks old) male C57Bl/6 mice underwent CCI at a 2-mm depth of deflection. Hematoxylin and eosin staining was performed 3 to 7 days after recovery from CCI, and injury volume was analyzed using ImageJ. Neurobehavioral characterization after CCI was performed using the Barnes maze test (BMT), passive avoidance test, open-field test, light/dark test, tail suspension test, and rotarod test. Acutely and subacutely (3 and 7 days after CCI, respectively), CCI mice showed graded injury compared to sham mice for all analyzed deflection depths. Results Time-dependent differences in injury volume were noted between 3 and 7 days following 2-mm TBI in adult mice. In the BMT, 2-mm TBI adults showed spatial memory deficits compared to sham adults (P<0.05). However, no difference in spatial learning and memory was found between sham and 2-mm CCI groups among pediatric mice. The open-field test, light/dark test, and tail suspension test did not reveal differences in anxiety-like behaviors in both age groups. Conclusion Our findings revealed a graded injury response in both age groups. The BMT was an efficient cognitive test for assessing spatial/non-spatial learning following CCI in adult mice; however, spatial learning impairments in pediatric mice could not be assessed.http://www.ceemjournal.org/upload/pdf/ceem-18-019.pdfbrain injuries, traumaticcontrolled cortical impactage differenceneurobehavioral differences |
| spellingShingle | Suk-Woo Lee Mun-Sun Jang Seong-Hae Jeong Hoon Kim Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact Clinical and Experimental Emergency Medicine brain injuries, traumatic controlled cortical impact age difference neurobehavioral differences |
| title | Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact |
| title_full | Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact |
| title_fullStr | Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact |
| title_full_unstemmed | Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact |
| title_short | Exploratory, cognitive, and depressive-like behaviors in adult and pediatric mice exposed to controlled cortical impact |
| title_sort | exploratory cognitive and depressive like behaviors in adult and pediatric mice exposed to controlled cortical impact |
| topic | brain injuries, traumatic controlled cortical impact age difference neurobehavioral differences |
| url | http://www.ceemjournal.org/upload/pdf/ceem-18-019.pdf |
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