Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1).
Low-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0274911 |
| _version_ | 1811229392398974976 |
|---|---|
| author | Gustavo Rosero Gisela Pattarone Ana Peñaherera Julia Pilz Joschka Bödecker Maximiliano Perez Roland Mertelsmann Betiana Lerner Marie Follo |
| author_facet | Gustavo Rosero Gisela Pattarone Ana Peñaherera Julia Pilz Joschka Bödecker Maximiliano Perez Roland Mertelsmann Betiana Lerner Marie Follo |
| author_sort | Gustavo Rosero |
| collection | DOAJ |
| description | Low-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-dependent interaction of drugs on a breast cancer cell line (BCC) cultured in chambered coverslips. The LDM studies were combined with cell staining in order to better characterize different cell states and cell death modes, including caspase-dependent apoptosis, caspase-independent cell death and autophagy-dependent cell death. Microscope images were examined using the Fiji Trainable Weka Segmentation plugin to analyse cell area in 7500 images showing different modes of cell death. Paclitaxel combined with LDM chemotherapy demonstrated a reduction in the area covered by live cells. In contrast, there was an induction of high levels of cell death due to caspase-dependent apoptosis. |
| first_indexed | 2024-04-12T10:14:10Z |
| format | Article |
| id | doaj.art-f3c21a122767445e8edf8bd5b407baf6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T10:14:10Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f3c21a122767445e8edf8bd5b407baf62022-12-22T03:37:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179e027491110.1371/journal.pone.0274911Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1).Gustavo RoseroGisela PattaroneAna PeñahereraJulia PilzJoschka BödeckerMaximiliano PerezRoland MertelsmannBetiana LernerMarie FolloLow-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-dependent interaction of drugs on a breast cancer cell line (BCC) cultured in chambered coverslips. The LDM studies were combined with cell staining in order to better characterize different cell states and cell death modes, including caspase-dependent apoptosis, caspase-independent cell death and autophagy-dependent cell death. Microscope images were examined using the Fiji Trainable Weka Segmentation plugin to analyse cell area in 7500 images showing different modes of cell death. Paclitaxel combined with LDM chemotherapy demonstrated a reduction in the area covered by live cells. In contrast, there was an induction of high levels of cell death due to caspase-dependent apoptosis.https://doi.org/10.1371/journal.pone.0274911 |
| spellingShingle | Gustavo Rosero Gisela Pattarone Ana Peñaherera Julia Pilz Joschka Bödecker Maximiliano Perez Roland Mertelsmann Betiana Lerner Marie Follo Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1). PLoS ONE |
| title | Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1). |
| title_full | Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1). |
| title_fullStr | Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1). |
| title_full_unstemmed | Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1). |
| title_short | Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1). |
| title_sort | metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells jimt 1 |
| url | https://doi.org/10.1371/journal.pone.0274911 |
| work_keys_str_mv | AT gustavorosero metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT giselapattarone metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT anapenaherera metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT juliapilz metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT joschkabodecker metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT maximilianoperez metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT rolandmertelsmann metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT betianalerner metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 AT mariefollo metronomicdosesanddrugschematiccombinationresponsetestedwithinchamberedcoverslipsforthetreatmentofbreastcancercellsjimt1 |