Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes
Abstract Background African swine fever (ASF) is a highly contagious and deadly viral disease affecting domestic and wild pigs of all ages. African swine fever virus (ASFV) has spread rapidly through Eastern and Southeastern Asia first appearing in Vietnam in 2019. Objectives Molecular typing of Afr...
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| Format: | Article |
| Language: | English |
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Wiley
2022-03-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.702 |
| _version_ | 1811271698087936000 |
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| author | Ha Thi Thanh Tran Anh Duc Truong Anh Kieu Dang Duc Viet Ly Nhu Thi Chu Tuan Van Hoang Huyen Thi Nguyen Christopher L Netherton Hoang Vu Dang |
| author_facet | Ha Thi Thanh Tran Anh Duc Truong Anh Kieu Dang Duc Viet Ly Nhu Thi Chu Tuan Van Hoang Huyen Thi Nguyen Christopher L Netherton Hoang Vu Dang |
| author_sort | Ha Thi Thanh Tran |
| collection | DOAJ |
| description | Abstract Background African swine fever (ASF) is a highly contagious and deadly viral disease affecting domestic and wild pigs of all ages. African swine fever virus (ASFV) has spread rapidly through Eastern and Southeastern Asia first appearing in Vietnam in 2019. Objectives Molecular typing of African swine fever virus (ASFV) in Vietnam has identified two principal variants circulating based on the sequencing of the intergenic region (IRG) between the I73R and I329L genes. Identification of additional genetic markers would enable higher resolution tracing of outbreaks within the country. Methods Sequence analysis suggested the IRG between the A179L and A137R genes may also exhibit variability, PCR primers were designed and samples from Vietnam were subject to Sanger sequencing. Results We developed a novel method for sub‐grouping of ASFV based on the IRG between the A179L and A137R genes of ASFV. Our results demonstrated that the finding of the insertion or deletion of an 11‐ nucleotide sequence (GATACAATTGT) between the A179L‐A137R genes. Conclusions The sub‐grouping method may provide useful insights into the evolution of genotype II ASFV as well as providing evidence of a relationship between geographically separated outbreaks. |
| first_indexed | 2024-04-12T22:25:40Z |
| format | Article |
| id | doaj.art-f3c67e9d079c41dba3d5a295928c0810 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-12T22:25:40Z |
| publishDate | 2022-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-f3c67e9d079c41dba3d5a295928c08102022-12-22T03:14:12ZengWileyVeterinary Medicine and Science2053-10952022-03-018260760910.1002/vms3.702Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genesHa Thi Thanh Tran0Anh Duc Truong1Anh Kieu Dang2Duc Viet Ly3Nhu Thi Chu4Tuan Van Hoang5Huyen Thi Nguyen6Christopher L Netherton7Hoang Vu Dang8Department of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamAfrican Swine Fever Vaccinology Group The Pirbright Institute Surrey UKDepartment of Biochemistry and Immunology National Institute of Veterinary Research Dong Da Hanoi VietnamAbstract Background African swine fever (ASF) is a highly contagious and deadly viral disease affecting domestic and wild pigs of all ages. African swine fever virus (ASFV) has spread rapidly through Eastern and Southeastern Asia first appearing in Vietnam in 2019. Objectives Molecular typing of African swine fever virus (ASFV) in Vietnam has identified two principal variants circulating based on the sequencing of the intergenic region (IRG) between the I73R and I329L genes. Identification of additional genetic markers would enable higher resolution tracing of outbreaks within the country. Methods Sequence analysis suggested the IRG between the A179L and A137R genes may also exhibit variability, PCR primers were designed and samples from Vietnam were subject to Sanger sequencing. Results We developed a novel method for sub‐grouping of ASFV based on the IRG between the A179L and A137R genes of ASFV. Our results demonstrated that the finding of the insertion or deletion of an 11‐ nucleotide sequence (GATACAATTGT) between the A179L‐A137R genes. Conclusions The sub‐grouping method may provide useful insights into the evolution of genotype II ASFV as well as providing evidence of a relationship between geographically separated outbreaks.https://doi.org/10.1002/vms3.702A137RA179LAfrican swine feversub‐groupingVietnam |
| spellingShingle | Ha Thi Thanh Tran Anh Duc Truong Anh Kieu Dang Duc Viet Ly Nhu Thi Chu Tuan Van Hoang Huyen Thi Nguyen Christopher L Netherton Hoang Vu Dang Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes Veterinary Medicine and Science A137R A179L African swine fever sub‐grouping Vietnam |
| title | Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes |
| title_full | Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes |
| title_fullStr | Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes |
| title_full_unstemmed | Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes |
| title_short | Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes |
| title_sort | novel method for sub grouping of genotype ii african swine fever viruses based on the intergenic region between the a179l and a137r genes |
| topic | A137R A179L African swine fever sub‐grouping Vietnam |
| url | https://doi.org/10.1002/vms3.702 |
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