Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis
Abstract Increasing evidence infers that some complex diseases are attributed to co-infection with multiple pathogens, such as shrimp white feces syndrome (WFS); however, there is a lack of experimental evidence to validate such causal link. This deficiency further impedes rational design of probiot...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-024-00509-5 |
| _version_ | 1797209516894322688 |
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| author | Haonan Sha Jiaqi Lu Jiong Chen Jinbo Xiong |
| author_facet | Haonan Sha Jiaqi Lu Jiong Chen Jinbo Xiong |
| author_sort | Haonan Sha |
| collection | DOAJ |
| description | Abstract Increasing evidence infers that some complex diseases are attributed to co-infection with multiple pathogens, such as shrimp white feces syndrome (WFS); however, there is a lack of experimental evidence to validate such causal link. This deficiency further impedes rational design of probiotics to elicit desired benefits to shrimp WFS resistance. Herein, we validated the causal roles of Vibrio fluvialis, V. coralliilyticus and V. tubiashii (in a ratio of 7:2:1) in shrimp WFS etiology, which fully satisfied Koch’s postulates. Correspondingly, we precisely designed four antagonistic strains: Ruegeria lacuscaerulensis, Nioella nitratireducens, Bacillus subtilis and Streptomyces euryhalinus in a ratio of 4:3:2:1, which efficiently guarded against WFS. Dietary supplementation of the probiotics stimulated beneficial gut populations, streptomycin, short chain fatty acids, taurine metabolism potentials, network stability, tight junction, and host selection, while reducing turnover rate and average variation degree of gut microbiota, thereby facilitating ecological and mechanical barriers against pathogens. Additionally, shrimp immune pathways, such as Fcγ R-mediated phagocytosis, Toll-like receptor and RIG-I-like receptor signaling pathways conferring immune barrier, were activated by probiotics supplementation. Collectively, we establish an updated framework for precisely validating co-infection with multiple pathogens and rationally designing antagonistic probiotics. Furthermore, our findings uncover the underlying beneficial mechanisms of designed probiotics from the probiotics–gut microbiome–host immunity axis. |
| first_indexed | 2024-04-24T09:55:57Z |
| format | Article |
| id | doaj.art-f3ce6895fdd94a4eb99eb34a6071ed19 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-04-24T09:55:57Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-f3ce6895fdd94a4eb99eb34a6071ed192024-04-14T11:09:13ZengNature Portfolionpj Biofilms and Microbiomes2055-50082024-04-0110111510.1038/s41522-024-00509-5Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axisHaonan Sha0Jiaqi Lu1Jiong Chen2Jinbo Xiong3State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Insititute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Insititute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Insititute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Insititute of Plant Virology, Ningbo UniversityAbstract Increasing evidence infers that some complex diseases are attributed to co-infection with multiple pathogens, such as shrimp white feces syndrome (WFS); however, there is a lack of experimental evidence to validate such causal link. This deficiency further impedes rational design of probiotics to elicit desired benefits to shrimp WFS resistance. Herein, we validated the causal roles of Vibrio fluvialis, V. coralliilyticus and V. tubiashii (in a ratio of 7:2:1) in shrimp WFS etiology, which fully satisfied Koch’s postulates. Correspondingly, we precisely designed four antagonistic strains: Ruegeria lacuscaerulensis, Nioella nitratireducens, Bacillus subtilis and Streptomyces euryhalinus in a ratio of 4:3:2:1, which efficiently guarded against WFS. Dietary supplementation of the probiotics stimulated beneficial gut populations, streptomycin, short chain fatty acids, taurine metabolism potentials, network stability, tight junction, and host selection, while reducing turnover rate and average variation degree of gut microbiota, thereby facilitating ecological and mechanical barriers against pathogens. Additionally, shrimp immune pathways, such as Fcγ R-mediated phagocytosis, Toll-like receptor and RIG-I-like receptor signaling pathways conferring immune barrier, were activated by probiotics supplementation. Collectively, we establish an updated framework for precisely validating co-infection with multiple pathogens and rationally designing antagonistic probiotics. Furthermore, our findings uncover the underlying beneficial mechanisms of designed probiotics from the probiotics–gut microbiome–host immunity axis.https://doi.org/10.1038/s41522-024-00509-5 |
| spellingShingle | Haonan Sha Jiaqi Lu Jiong Chen Jinbo Xiong Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis npj Biofilms and Microbiomes |
| title | Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis |
| title_full | Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis |
| title_fullStr | Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis |
| title_full_unstemmed | Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis |
| title_short | Rationally designed probiotics prevent shrimp white feces syndrome via the probiotics–gut microbiome–immunity axis |
| title_sort | rationally designed probiotics prevent shrimp white feces syndrome via the probiotics gut microbiome immunity axis |
| url | https://doi.org/10.1038/s41522-024-00509-5 |
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