Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis
Peritendinous adhesions are a frequent occurrence following tenolysis and present a major clinical challenge regarding prevention and management, with no recovery assured from conservative or surgical approaches. Herein, we investigated the effectiveness of Hyaloglide<sup>®</sup>, a hyal...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/16/7613 |
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| author | Andrea Marchesini Francesco De Francesco Pier Paolo Pangrazi Letizia Senesi Andrea Campodonico Valentina Riccio Stefano Geuna Barbara Zavan Michele Riccio |
| author_facet | Andrea Marchesini Francesco De Francesco Pier Paolo Pangrazi Letizia Senesi Andrea Campodonico Valentina Riccio Stefano Geuna Barbara Zavan Michele Riccio |
| author_sort | Andrea Marchesini |
| collection | DOAJ |
| description | Peritendinous adhesions are a frequent occurrence following tenolysis and present a major clinical challenge regarding prevention and management, with no recovery assured from conservative or surgical approaches. Herein, we investigated the effectiveness of Hyaloglide<sup>®</sup>, a hyaluronan gel-based product with a novel autocross-linked technology, in a rabbit model affected by tenolysis on the flexor digitorum communis tendon (FDC). A 1.5-cm-long scrubbing of the tendon surface was performed bilaterally to induce peritendinous adhesion on FDC of 30 animals with subsequent application of Hyaloglide<sup>®</sup> on the surrounding injured area, in one randomly chosen tendon. The contralateral tendon was treated with saline solution as the control. We sacrificed the rabbit models after 45 days of surgery and quantitatively assessed the generation of peritendinous adherence and regeneration of the tendon sheaths using histological (hematossyline-eosine, masson’s trichromic), histomorphometrical (Tang score, Soslowsky Svesson, and Cook score), light electron microscopic, and gene expression investigations. Four rabbits were devoted to biomechanical analysis. Peritendinous adhesions were limited in Hyaloglide<sup>®</sup>-treated tendons; moreover, well-regenerated tendon sheaths were observed conversely to untreated tendons presenting with extensive areas of adhesions on the tendon surface. Histomorphometrical analysis revealed an adhesion score (Tang score) significantly better in the treated group (<i>p</i> = 0.001 *) compared to the control group. Moreover, the Soslowsky, Svensson, and Cook score parameters revealed a significantly improved regeneration for fiber structure, cellularity, and vascularity in the treated group (<i>p</i> = 0.001 *). No differences were reported for cartilaginous formation (<i>p</i> = 0.08). Gene expression analysis showed a significant increase in collagen type I expression in the treated group compared to the control group, while metalloprotease 1 and 9 were significantly increased in the control group. Biomechanical analysis did not show significant differences in both groups. Hyaloglide<sup>®</sup> treatment was safe and well-tolerated, generating improved tissue status. Local application of Hyaloglide<sup>®</sup> prevents adhesion formation after tenolysis and promotes normal healing with regeneration of the synovial sheath in a rabbit model. |
| first_indexed | 2024-03-10T09:01:54Z |
| format | Article |
| id | doaj.art-f3d1376bbfa846df9221fab38eae98d7 |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T09:01:54Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-f3d1376bbfa846df9221fab38eae98d72023-11-22T06:44:30ZengMDPI AGApplied Sciences2076-34172021-08-011116761310.3390/app11167613Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following TenolysisAndrea Marchesini0Francesco De Francesco1Pier Paolo Pangrazi2Letizia Senesi3Andrea Campodonico4Valentina Riccio5Stefano Geuna6Barbara Zavan7Michele Riccio8Department of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda ‘Ospedali Riuniti’, 60126 Ancona, ItalyDepartment of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda ‘Ospedali Riuniti’, 60126 Ancona, ItalyDepartment of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda ‘Ospedali Riuniti’, 60126 Ancona, ItalyDepartment of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda ‘Ospedali Riuniti’, 60126 Ancona, ItalyDepartment of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda ‘Ospedali Riuniti’, 60126 Ancona, ItalyVeterinary Medical School, University of Camerino, 62024 Camerino, ItalyDepartment of Clinical and Biological Sciences & Neuroscience Institute of the Cavalieri Ottolenghi Foundation, University of Turin, 10124 Turin, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda ‘Ospedali Riuniti’, 60126 Ancona, ItalyPeritendinous adhesions are a frequent occurrence following tenolysis and present a major clinical challenge regarding prevention and management, with no recovery assured from conservative or surgical approaches. Herein, we investigated the effectiveness of Hyaloglide<sup>®</sup>, a hyaluronan gel-based product with a novel autocross-linked technology, in a rabbit model affected by tenolysis on the flexor digitorum communis tendon (FDC). A 1.5-cm-long scrubbing of the tendon surface was performed bilaterally to induce peritendinous adhesion on FDC of 30 animals with subsequent application of Hyaloglide<sup>®</sup> on the surrounding injured area, in one randomly chosen tendon. The contralateral tendon was treated with saline solution as the control. We sacrificed the rabbit models after 45 days of surgery and quantitatively assessed the generation of peritendinous adherence and regeneration of the tendon sheaths using histological (hematossyline-eosine, masson’s trichromic), histomorphometrical (Tang score, Soslowsky Svesson, and Cook score), light electron microscopic, and gene expression investigations. Four rabbits were devoted to biomechanical analysis. Peritendinous adhesions were limited in Hyaloglide<sup>®</sup>-treated tendons; moreover, well-regenerated tendon sheaths were observed conversely to untreated tendons presenting with extensive areas of adhesions on the tendon surface. Histomorphometrical analysis revealed an adhesion score (Tang score) significantly better in the treated group (<i>p</i> = 0.001 *) compared to the control group. Moreover, the Soslowsky, Svensson, and Cook score parameters revealed a significantly improved regeneration for fiber structure, cellularity, and vascularity in the treated group (<i>p</i> = 0.001 *). No differences were reported for cartilaginous formation (<i>p</i> = 0.08). Gene expression analysis showed a significant increase in collagen type I expression in the treated group compared to the control group, while metalloprotease 1 and 9 were significantly increased in the control group. Biomechanical analysis did not show significant differences in both groups. Hyaloglide<sup>®</sup> treatment was safe and well-tolerated, generating improved tissue status. Local application of Hyaloglide<sup>®</sup> prevents adhesion formation after tenolysis and promotes normal healing with regeneration of the synovial sheath in a rabbit model.https://www.mdpi.com/2076-3417/11/16/7613tendon injuriestenolysishistologyanimal modelhyaluronan gelautocross-linked gel |
| spellingShingle | Andrea Marchesini Francesco De Francesco Pier Paolo Pangrazi Letizia Senesi Andrea Campodonico Valentina Riccio Stefano Geuna Barbara Zavan Michele Riccio Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis Applied Sciences tendon injuries tenolysis histology animal model hyaluronan gel autocross-linked gel |
| title | Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis |
| title_full | Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis |
| title_fullStr | Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis |
| title_full_unstemmed | Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis |
| title_short | Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tenolysis |
| title_sort | effectiveness of hyaluronan autocross linked based gel in the prevention of peritendinous adherence following tenolysis |
| topic | tendon injuries tenolysis histology animal model hyaluronan gel autocross-linked gel |
| url | https://www.mdpi.com/2076-3417/11/16/7613 |
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