Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study
This study aimed to evaluate the correlation between epidermal growth factor (EGF) and receptor (EGFR) levels in different clinical stages of dental implant rehabilitation and trace mucositis development’s biological profile. Thirty-six participants from the Specialization in Implant Dentistry, Univ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Healthcare |
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| Online Access: | https://www.mdpi.com/2227-9032/9/10/1277 |
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| author | José Jorge Schoichet Carlos Fernando de Almeida Barros Mourão Edgard de Mello Fonseca Carlos Ramirez Ricardo Villas-Boas Juliana Prazeres Valquiria Quinelato Telma Regina Aguiar Marina Prado Angelo Cardarelli Rafael Mello-Machado Priscila Casado |
| author_facet | José Jorge Schoichet Carlos Fernando de Almeida Barros Mourão Edgard de Mello Fonseca Carlos Ramirez Ricardo Villas-Boas Juliana Prazeres Valquiria Quinelato Telma Regina Aguiar Marina Prado Angelo Cardarelli Rafael Mello-Machado Priscila Casado |
| author_sort | José Jorge Schoichet |
| collection | DOAJ |
| description | This study aimed to evaluate the correlation between epidermal growth factor (EGF) and receptor (EGFR) levels in different clinical stages of dental implant rehabilitation and trace mucositis development’s biological profile. Thirty-six participants from the Specialization in Implant Dentistry, Universidade Federal Fluminense, Brazil, were included in the study and underwent sample collection: inside the alveolar socket, immediately before implant placement (Group 1, <i>n</i> = 10); at the peri-implant crevicular fluid (PICF) during reopening (Group 2, <i>n</i> = 10); PICF from healthy peri-implant in function (Group 3, <i>n</i> = 8); and PICF from mucositis sites (Group 4, <i>n</i> = 18). Quantitative polymerase chain reaction (PCR) evaluated EGF/EGFR gene expression using the SYBR Green Master Mix detection system. The results showed that EGF expression in the peri-implant crevicular fluid was statistically different. There was a higher EGF expression for group C (peri-implant health) (<i>p</i> = 0.04) than for the other groups. Regarding EGFR, there was no statistical difference among the groups (<i>p</i> = 0.56). It was concluded that low levels of EGF gene expression in the peri-implant crevicular fluid are related to the development of peri-implant mucositis and the absence of mucosae sealing. There was no correlation between EGFR gene expression with health or mucositis. |
| first_indexed | 2024-03-10T06:32:17Z |
| format | Article |
| id | doaj.art-f3d6169396314e63a1ff722c04745591 |
| institution | Directory Open Access Journal |
| issn | 2227-9032 |
| language | English |
| last_indexed | 2024-03-10T06:32:17Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Healthcare |
| spelling | doaj.art-f3d6169396314e63a1ff722c047455912023-11-22T18:24:28ZengMDPI AGHealthcare2227-90322021-09-01910127710.3390/healthcare9101277Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot StudyJosé Jorge Schoichet0Carlos Fernando de Almeida Barros Mourão1Edgard de Mello Fonseca2Carlos Ramirez3Ricardo Villas-Boas4Juliana Prazeres5Valquiria Quinelato6Telma Regina Aguiar7Marina Prado8Angelo Cardarelli9Rafael Mello-Machado10Priscila Casado11Graduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilClinical Research Laboratory in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilDepartment of Dentistry, University Vita-Salute San Raffaele, 20100 Milan, ItalyClinical Research Laboratory in Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilGraduate Program in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Niteroi 24020-140, RJ, BrazilThis study aimed to evaluate the correlation between epidermal growth factor (EGF) and receptor (EGFR) levels in different clinical stages of dental implant rehabilitation and trace mucositis development’s biological profile. Thirty-six participants from the Specialization in Implant Dentistry, Universidade Federal Fluminense, Brazil, were included in the study and underwent sample collection: inside the alveolar socket, immediately before implant placement (Group 1, <i>n</i> = 10); at the peri-implant crevicular fluid (PICF) during reopening (Group 2, <i>n</i> = 10); PICF from healthy peri-implant in function (Group 3, <i>n</i> = 8); and PICF from mucositis sites (Group 4, <i>n</i> = 18). Quantitative polymerase chain reaction (PCR) evaluated EGF/EGFR gene expression using the SYBR Green Master Mix detection system. The results showed that EGF expression in the peri-implant crevicular fluid was statistically different. There was a higher EGF expression for group C (peri-implant health) (<i>p</i> = 0.04) than for the other groups. Regarding EGFR, there was no statistical difference among the groups (<i>p</i> = 0.56). It was concluded that low levels of EGF gene expression in the peri-implant crevicular fluid are related to the development of peri-implant mucositis and the absence of mucosae sealing. There was no correlation between EGFR gene expression with health or mucositis.https://www.mdpi.com/2227-9032/9/10/1277epidermal growth factormucositisperi-implant tissuemucosae sealing |
| spellingShingle | José Jorge Schoichet Carlos Fernando de Almeida Barros Mourão Edgard de Mello Fonseca Carlos Ramirez Ricardo Villas-Boas Juliana Prazeres Valquiria Quinelato Telma Regina Aguiar Marina Prado Angelo Cardarelli Rafael Mello-Machado Priscila Casado Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study Healthcare epidermal growth factor mucositis peri-implant tissue mucosae sealing |
| title | Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study |
| title_full | Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study |
| title_fullStr | Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study |
| title_full_unstemmed | Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study |
| title_short | Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study |
| title_sort | epidermal growth factor is associated with loss of mucosae sealing and peri implant mucositis a pilot study |
| topic | epidermal growth factor mucositis peri-implant tissue mucosae sealing |
| url | https://www.mdpi.com/2227-9032/9/10/1277 |
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