Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches
Objective: The aim of this study is to detect the prevalence and diagnosis of molecular characteristics of Cryptosporidium infection in handlers of domestic pigeons in Baghdad City. Traditional and molecular diagnostic methods were employed to detect and identify Cryptosporidium species. Materials a...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Network for the Veterinarians of Bangladesh
2023-12-01
|
| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | https://bdvets.org/JAVAR/?mno=171713 |
| _version_ | 1797341492505739264 |
|---|---|
| author | Yahya F. Hashim Mohammed Th. S. Al-Zubaidi |
| author_facet | Yahya F. Hashim Mohammed Th. S. Al-Zubaidi |
| author_sort | Yahya F. Hashim |
| collection | DOAJ |
| description | Objective: The aim of this study is to detect the prevalence and diagnosis of molecular characteristics of Cryptosporidium infection in handlers of domestic pigeons in Baghdad City. Traditional and molecular diagnostic methods were employed to detect and identify Cryptosporidium species.
Materials and methods: Sixty stool samples were collected from the handlers of domestic pigeons, and various techniques, including direct smear, flotation concentration, staining methods, and DNA extraction coupled with nested polymerase chain reaction (PCR), were utilized.
Results: The results obtained from traditional methods indicated an overall infection rate of 55% in handlers of domestic pigeons, while significant variations were noted between male and female handlers. Age group 21–40 years were found to have higher infection rates were found to have higher infection rates. The prevalence of Cryptosporidium also displayed temporal variability throughout the study period. Molecular analysis using nested PCR revealed higher infection rates of 86% in handlers of domestic pigeon samples. Genotyping and phylogenetic analysis identified the presence of Cryptosporidium parvam species in handlers of domestic pigeons, indicating zoonotic transmission potential.
Conclusion: These findings underscore the high prevalence of Cryptosporidium infection among handlers of domestic pigeons in Baghdad City. [J Adv Vet Anim Res 2023; 10(4.000): 744-750] |
| first_indexed | 2024-03-08T10:20:16Z |
| format | Article |
| id | doaj.art-f3d687bc4c874927a030ab6f825994af |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-03-08T10:20:16Z |
| publishDate | 2023-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-f3d687bc4c874927a030ab6f825994af2024-01-28T04:10:18ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102023-12-0110474475010.5455/javar.2023.j730171713Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approachesYahya F. Hashim0Mohammed Th. S. Al-Zubaidi1Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Objective: The aim of this study is to detect the prevalence and diagnosis of molecular characteristics of Cryptosporidium infection in handlers of domestic pigeons in Baghdad City. Traditional and molecular diagnostic methods were employed to detect and identify Cryptosporidium species. Materials and methods: Sixty stool samples were collected from the handlers of domestic pigeons, and various techniques, including direct smear, flotation concentration, staining methods, and DNA extraction coupled with nested polymerase chain reaction (PCR), were utilized. Results: The results obtained from traditional methods indicated an overall infection rate of 55% in handlers of domestic pigeons, while significant variations were noted between male and female handlers. Age group 21–40 years were found to have higher infection rates were found to have higher infection rates. The prevalence of Cryptosporidium also displayed temporal variability throughout the study period. Molecular analysis using nested PCR revealed higher infection rates of 86% in handlers of domestic pigeon samples. Genotyping and phylogenetic analysis identified the presence of Cryptosporidium parvam species in handlers of domestic pigeons, indicating zoonotic transmission potential. Conclusion: These findings underscore the high prevalence of Cryptosporidium infection among handlers of domestic pigeons in Baghdad City. [J Adv Vet Anim Res 2023; 10(4.000): 744-750]https://bdvets.org/JAVAR/?mno=171713baghdad city; cryptosporidium; domestic pigeons; handlers; nested pcr |
| spellingShingle | Yahya F. Hashim Mohammed Th. S. Al-Zubaidi Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches Journal of Advanced Veterinary and Animal Research baghdad city; cryptosporidium; domestic pigeons; handlers; nested pcr |
| title | Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches |
| title_full | Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches |
| title_fullStr | Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches |
| title_full_unstemmed | Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches |
| title_short | Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches |
| title_sort | cryptosporidium species diagnosis in handlers of domestic pigeons in baghdad city molecular and microscopic approaches |
| topic | baghdad city; cryptosporidium; domestic pigeons; handlers; nested pcr |
| url | https://bdvets.org/JAVAR/?mno=171713 |
| work_keys_str_mv | AT yahyafhashim cryptosporidiumspeciesdiagnosisinhandlersofdomesticpigeonsinbaghdadcitymolecularandmicroscopicapproaches AT mohammedthsalzubaidi cryptosporidiumspeciesdiagnosisinhandlersofdomesticpigeonsinbaghdadcitymolecularandmicroscopicapproaches |