Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease
von Willebrand factor A (vWA) genes are well characterized in humans except for few BONZAI genes, but the vWA genes are least explored in plants. Considering the novelty and vital role of vWA genes, this study aimed at characterization of vWA superfamily in rice. Rice genome was found to have 40 vWA...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630822000439 |
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| author | Suhas Gorakh Karkute Vishesh Kumar Mohd Tasleem Dwijesh Chandra Mishra Krishna Kumar Chaturvedi Anil Rai Amitha Mithra Sevanthi Kishor Gaikwad Tilak Raj Sharma Amolkumar U. Solanke |
| author_facet | Suhas Gorakh Karkute Vishesh Kumar Mohd Tasleem Dwijesh Chandra Mishra Krishna Kumar Chaturvedi Anil Rai Amitha Mithra Sevanthi Kishor Gaikwad Tilak Raj Sharma Amolkumar U. Solanke |
| author_sort | Suhas Gorakh Karkute |
| collection | DOAJ |
| description | von Willebrand factor A (vWA) genes are well characterized in humans except for few BONZAI genes, but the vWA genes are least explored in plants. Considering the novelty and vital role of vWA genes, this study aimed at characterization of vWA superfamily in rice. Rice genome was found to have 40 vWA genes distributed across all the 12 chromosomes, and 20 of the 40 vWA genes were unique while the remaining shared large fragment similarities with each other, indicating gene duplication. In addition to vWA domain, vWA proteins possess other different motifs or domains, such as ubiquitin interacting motif in protein degradation pathway, and RING finger in protein-protein interaction. Expression analysis of vWA genes in available expression data suggested that they probably function in biotic and abiotic stress responses including hormonal response and signaling. The frequency of transposon elements in the entire 3K rice germplasm was negligible except for 9 vWA genes, indicating the importance of these genes in rice. Structural and functional diversities showed that the vWA genes in a blast-resistant rice variety Tetep had huge variations compared to blast-susceptible rice varieties HP2216 and Nipponbare. qRT-PCR analysis of vWA genes in Magnaporthe oryzae infected rice tissues indicated OsvWA9, OsvWA36, OsvWA37 and OsvWA18 as the optimal candidate genes for disease resistance. This is the first attempt to characterize vWA gene family in plant species. |
| first_indexed | 2024-12-12T07:34:38Z |
| format | Article |
| id | doaj.art-f3d8c8209d3c468fa4b275a9163439c5 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-12T07:34:38Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-f3d8c8209d3c468fa4b275a9163439c52022-12-22T00:32:56ZengElsevierRice Science1672-63082022-07-01294375384Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast DiseaseSuhas Gorakh Karkute0Vishesh Kumar1Mohd Tasleem2Dwijesh Chandra Mishra3Krishna Kumar Chaturvedi4Anil Rai5Amitha Mithra Sevanthi6Kishor Gaikwad7Tilak Raj Sharma8Amolkumar U. Solanke9Indian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, IndiaIndian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, IndiaIndian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, IndiaICAR-Indian Agricultural Statistics Research Institute, New Delhi 110012, IndiaICAR-Indian Agricultural Statistics Research Institute, New Delhi 110012, IndiaICAR-Indian Agricultural Statistics Research Institute, New Delhi 110012, IndiaIndian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, IndiaIndian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, IndiaICAR-Division of Crop Science, New Delhi 110001, IndiaIndian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, India; Corresponding author: Indian Council of Agricultural Research (ICAR)-National Institute for Plant Biotechnology, New Delhi 110012, India.von Willebrand factor A (vWA) genes are well characterized in humans except for few BONZAI genes, but the vWA genes are least explored in plants. Considering the novelty and vital role of vWA genes, this study aimed at characterization of vWA superfamily in rice. Rice genome was found to have 40 vWA genes distributed across all the 12 chromosomes, and 20 of the 40 vWA genes were unique while the remaining shared large fragment similarities with each other, indicating gene duplication. In addition to vWA domain, vWA proteins possess other different motifs or domains, such as ubiquitin interacting motif in protein degradation pathway, and RING finger in protein-protein interaction. Expression analysis of vWA genes in available expression data suggested that they probably function in biotic and abiotic stress responses including hormonal response and signaling. The frequency of transposon elements in the entire 3K rice germplasm was negligible except for 9 vWA genes, indicating the importance of these genes in rice. Structural and functional diversities showed that the vWA genes in a blast-resistant rice variety Tetep had huge variations compared to blast-susceptible rice varieties HP2216 and Nipponbare. qRT-PCR analysis of vWA genes in Magnaporthe oryzae infected rice tissues indicated OsvWA9, OsvWA36, OsvWA37 and OsvWA18 as the optimal candidate genes for disease resistance. This is the first attempt to characterize vWA gene family in plant species.http://www.sciencedirect.com/science/article/pii/S1672630822000439von Willebrand factor Abiotic stressabiotic stressrice blast diseaseMagnaporthe oryzae |
| spellingShingle | Suhas Gorakh Karkute Vishesh Kumar Mohd Tasleem Dwijesh Chandra Mishra Krishna Kumar Chaturvedi Anil Rai Amitha Mithra Sevanthi Kishor Gaikwad Tilak Raj Sharma Amolkumar U. Solanke Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease Rice Science von Willebrand factor A biotic stress abiotic stress rice blast disease Magnaporthe oryzae |
| title | Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease |
| title_full | Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease |
| title_fullStr | Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease |
| title_full_unstemmed | Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease |
| title_short | Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease |
| title_sort | genome wide analysis of von willebrand factor a gene family in rice for its role in imparting biotic stress resistance with emphasis on rice blast disease |
| topic | von Willebrand factor A biotic stress abiotic stress rice blast disease Magnaporthe oryzae |
| url | http://www.sciencedirect.com/science/article/pii/S1672630822000439 |
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