PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE
Laccase has been obtained through two different production systems, the first using Pleurotus ostreatus in solid state fermentation, the second one using Trametes versicolor in submerged culture. Different substrates (by products from yeast, flour and beverage industries) have been evaluated in both...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Colombiano del Petróleo (ICP) - ECOPETROL S.A.
2002-12-01
|
| Series: | CT&F Ciencia, Tecnología & Futuro |
| Subjects: | |
| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-53832002000100002 |
| _version_ | 1811181428933656576 |
|---|---|
| author | María-Carolina Vargas Nubia E Ramírez |
| author_facet | María-Carolina Vargas Nubia E Ramírez |
| author_sort | María-Carolina Vargas |
| collection | DOAJ |
| description | Laccase has been obtained through two different production systems, the first using Pleurotus ostreatus in solid state fermentation, the second one using Trametes versicolor in submerged culture. Different substrates (by products from yeast, flour and beverage industries) have been evaluated in both systems. Maximum laccase yield with Pleurotus ostreatus (25 U/ml) was obtained in a wheat bran medium. The maximum enzyme concentration level using Trametes versicolor (25 U/ml) was achieved in a submerged system containing 10% vinasse, 4,5% wheat bran and 0,2% molasses per liter of waste. Culture filtrate extracted from Pleurotus ostreatus was used to remove phenol from wastewater. The enzymatic treatment is effective over a wide pH and temperature range. The laccase treatment has been successfully used to dephenolize industrial petrol refinery wastewater. The advantage of laccase dephenolization is that this enzyme uses molecular oxygen as an oxidant.<br>La lacasa se ha obtenido a través de dos sistemas de producción diferentes, el primero usando Pleurotus ostreatus en fermentación en estado sólido, el segundo usando Trametes versicolor en cultivo sumergido. Se han evaluado diferentes sustratos (derivados de la levadura, harina e industrias de la bebida) en ambos sistemas. La mayor cantidad de la lacasa producida con Pleurotus ostreatus (25 U/ml) se obtuvo en un medio de salvado de trigo. El máximo nivel de concentración de la enzima usando Trametes versicolor (25 U/ml) se logró en un sistema sumergido con 10% vinaza, 4,5% salvado de trigo y 0,2% de melaza por litro. El filtrado del cultivo extraído del Pleurotus ostreatus se usó para remover el fenol de las aguas residuales. El tratamiento enzimático es eficaz para un amplio rango de pH y de temperatura. El tratamiento con la lacasa se ha utilizado para desfenolizar el agua residual de las refinerías de la industria del petróleo. La ventaja de la desfenolización lacasa es que esta enzima usa el oxígeno molecular como un oxidante. |
| first_indexed | 2024-04-11T09:17:40Z |
| format | Article |
| id | doaj.art-f3db38ed492a40e39c178106230f423c |
| institution | Directory Open Access Journal |
| issn | 0122-5383 |
| language | English |
| last_indexed | 2024-04-11T09:17:40Z |
| publishDate | 2002-12-01 |
| publisher | Instituto Colombiano del Petróleo (ICP) - ECOPETROL S.A. |
| record_format | Article |
| series | CT&F Ciencia, Tecnología & Futuro |
| spelling | doaj.art-f3db38ed492a40e39c178106230f423c2022-12-22T04:32:18ZengInstituto Colombiano del Petróleo (ICP) - ECOPETROL S.A.CT&F Ciencia, Tecnología & Futuro0122-53832002-12-01232330PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASEMaría-Carolina VargasNubia E RamírezLaccase has been obtained through two different production systems, the first using Pleurotus ostreatus in solid state fermentation, the second one using Trametes versicolor in submerged culture. Different substrates (by products from yeast, flour and beverage industries) have been evaluated in both systems. Maximum laccase yield with Pleurotus ostreatus (25 U/ml) was obtained in a wheat bran medium. The maximum enzyme concentration level using Trametes versicolor (25 U/ml) was achieved in a submerged system containing 10% vinasse, 4,5% wheat bran and 0,2% molasses per liter of waste. Culture filtrate extracted from Pleurotus ostreatus was used to remove phenol from wastewater. The enzymatic treatment is effective over a wide pH and temperature range. The laccase treatment has been successfully used to dephenolize industrial petrol refinery wastewater. The advantage of laccase dephenolization is that this enzyme uses molecular oxygen as an oxidant.<br>La lacasa se ha obtenido a través de dos sistemas de producción diferentes, el primero usando Pleurotus ostreatus en fermentación en estado sólido, el segundo usando Trametes versicolor en cultivo sumergido. Se han evaluado diferentes sustratos (derivados de la levadura, harina e industrias de la bebida) en ambos sistemas. La mayor cantidad de la lacasa producida con Pleurotus ostreatus (25 U/ml) se obtuvo en un medio de salvado de trigo. El máximo nivel de concentración de la enzima usando Trametes versicolor (25 U/ml) se logró en un sistema sumergido con 10% vinaza, 4,5% salvado de trigo y 0,2% de melaza por litro. El filtrado del cultivo extraído del Pleurotus ostreatus se usó para remover el fenol de las aguas residuales. El tratamiento enzimático es eficaz para un amplio rango de pH y de temperatura. El tratamiento con la lacasa se ha utilizado para desfenolizar el agua residual de las refinerías de la industria del petróleo. La ventaja de la desfenolización lacasa es que esta enzima usa el oxígeno molecular como un oxidante.http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-53832002000100002fenollacasaagua residualsubstrato industrialfermentación en estado sólidoPhenollaccasewastewaterindustrial substratesolid state fermentation |
| spellingShingle | María-Carolina Vargas Nubia E Ramírez PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE CT&F Ciencia, Tecnología & Futuro fenol lacasa agua residual substrato industrial fermentación en estado sólido Phenol laccase wastewater industrial substrate solid state fermentation |
| title | PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE |
| title_full | PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE |
| title_fullStr | PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE |
| title_full_unstemmed | PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE |
| title_short | PHENOL OXIDATION OF PETROL REFINERY WASTEWATER CATALYZED BY LACCASE |
| title_sort | phenol oxidation of petrol refinery wastewater catalyzed by laccase |
| topic | fenol lacasa agua residual substrato industrial fermentación en estado sólido Phenol laccase wastewater industrial substrate solid state fermentation |
| url | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-53832002000100002 |
| work_keys_str_mv | AT mariacarolinavargas phenoloxidationofpetrolrefinerywastewatercatalyzedbylaccase AT nubiaeramirez phenoloxidationofpetrolrefinerywastewatercatalyzedbylaccase |