Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery
Strawberry tree (Arbutus unedo) is a small resilient species with a circum-Mediterranean distribution, high ecological relevance in southern European forests and with several economical applications. As most orchards are usually installed on marginal lands where plants usually face severe drought, s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1011542/full |
| _version_ | 1811319073916583936 |
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| author | João Martins Pierre Pétriacq Pierre Pétriacq Amélie Flandin Amélie Flandin Aurelio Gómez-Cadenas Pedro Monteiro Glória Pinto Jorge Canhoto |
| author_facet | João Martins Pierre Pétriacq Pierre Pétriacq Amélie Flandin Amélie Flandin Aurelio Gómez-Cadenas Pedro Monteiro Glória Pinto Jorge Canhoto |
| author_sort | João Martins |
| collection | DOAJ |
| description | Strawberry tree (Arbutus unedo) is a small resilient species with a circum-Mediterranean distribution, high ecological relevance in southern European forests and with several economical applications. As most orchards are usually installed on marginal lands where plants usually face severe drought, selecting plants that can better cope with water restriction is critical, and a better understanding of the tolerance mechanisms is required. Strawberry tree plants under drought follow a typical isohydric strategy, by limiting transpiration through stomata closure. However, the contribution of genotype and its bio-geographic origin on plant performance needs clarification, as well as the involvement of a specific metabolic reactions associated with the mechanical response. To test this hypothesis, several eco-physiological and biochemical parameters were assessed on different genotypes, and the metabolic profiles studied, including important stress-related phytohormones, on plants under different water regimes (plants watered to 70% and 18% field capacity) and a recovery assay. A contrasting drought tolerance was found in plants from different genotypes, associated with physiological and metabolic responses. Metabolomics revealed more than 500 metabolic features were differentially accumulated, including abscisic and salicylic acids, for the genotype with better performance under drought (A4). This genotype also recovered faster when the imposed stress was interrupted, thus indicating the relevance of metabolic adaptation under water deficit conditions. By correlating carbon assimilation with identified metabolites, some proved to be satisfactory predictors of plant performance under drought and might be used for marker assisted breeding. Therefore, our study proves the importance of genotype as a major selection criterion of resistant plants to drought and provides empirical knowledge of the metabolic response involved. We also hypothesized the involvement of phenolics on response mechanisms under drought, which is worth to be explored to shed light on the metabolic pathways involved in plant response to water stress. |
| first_indexed | 2024-04-13T12:36:50Z |
| format | Article |
| id | doaj.art-f3db7ffb58ba4c2bbfd93a8837a482c7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T12:36:50Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f3db7ffb58ba4c2bbfd93a8837a482c72022-12-22T02:46:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10115421011542Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recoveryJoão Martins0Pierre Pétriacq1Pierre Pétriacq2Amélie Flandin3Amélie Flandin4Aurelio Gómez-Cadenas5Pedro Monteiro6Glória Pinto7Jorge Canhoto8Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, Coimbra, PortugalUMR BFP, University Bordeaux, INRAE, Villenave d’Ornon, FranceBordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Villenave d’Ornon, FranceUMR BFP, University Bordeaux, INRAE, Villenave d’Ornon, FranceBordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Villenave d’Ornon, FranceDepartamento de Ciencias Agrarias y del Medio Natural, Universitat Jaume I, Castelló, SpainCentre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Aveiro, PortugalCentre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Aveiro, PortugalCentre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, Coimbra, PortugalStrawberry tree (Arbutus unedo) is a small resilient species with a circum-Mediterranean distribution, high ecological relevance in southern European forests and with several economical applications. As most orchards are usually installed on marginal lands where plants usually face severe drought, selecting plants that can better cope with water restriction is critical, and a better understanding of the tolerance mechanisms is required. Strawberry tree plants under drought follow a typical isohydric strategy, by limiting transpiration through stomata closure. However, the contribution of genotype and its bio-geographic origin on plant performance needs clarification, as well as the involvement of a specific metabolic reactions associated with the mechanical response. To test this hypothesis, several eco-physiological and biochemical parameters were assessed on different genotypes, and the metabolic profiles studied, including important stress-related phytohormones, on plants under different water regimes (plants watered to 70% and 18% field capacity) and a recovery assay. A contrasting drought tolerance was found in plants from different genotypes, associated with physiological and metabolic responses. Metabolomics revealed more than 500 metabolic features were differentially accumulated, including abscisic and salicylic acids, for the genotype with better performance under drought (A4). This genotype also recovered faster when the imposed stress was interrupted, thus indicating the relevance of metabolic adaptation under water deficit conditions. By correlating carbon assimilation with identified metabolites, some proved to be satisfactory predictors of plant performance under drought and might be used for marker assisted breeding. Therefore, our study proves the importance of genotype as a major selection criterion of resistant plants to drought and provides empirical knowledge of the metabolic response involved. We also hypothesized the involvement of phenolics on response mechanisms under drought, which is worth to be explored to shed light on the metabolic pathways involved in plant response to water stress.https://www.frontiersin.org/articles/10.3389/fpls.2022.1011542/fulldroughtphenolic compoundsmicropropagationplant hormonesstrawberry tree |
| spellingShingle | João Martins Pierre Pétriacq Pierre Pétriacq Amélie Flandin Amélie Flandin Aurelio Gómez-Cadenas Pedro Monteiro Glória Pinto Jorge Canhoto Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery Frontiers in Plant Science drought phenolic compounds micropropagation plant hormones strawberry tree |
| title | Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery |
| title_full | Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery |
| title_fullStr | Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery |
| title_full_unstemmed | Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery |
| title_short | Genotype determines Arbutus unedo L. physiological and metabolomic responses to drought and recovery |
| title_sort | genotype determines arbutus unedo l physiological and metabolomic responses to drought and recovery |
| topic | drought phenolic compounds micropropagation plant hormones strawberry tree |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1011542/full |
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