RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation. Here, we report instead that acute depletion o...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/83810 |
| _version_ | 1797829310363467776 |
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| author | James ZJ Kwan Thomas F Nguyen Anuli C Uzozie Marek A Budzynski Jieying Cui Joseph MC Lee Filip Van Petegem Philipp F Lange Sheila S Teves |
| author_facet | James ZJ Kwan Thomas F Nguyen Anuli C Uzozie Marek A Budzynski Jieying Cui Joseph MC Lee Filip Van Petegem Philipp F Lange Sheila S Teves |
| author_sort | James ZJ Kwan |
| collection | DOAJ |
| description | Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation. Here, we report instead that acute depletion of TBP in mouse embryonic stem cells has no global effect on ongoing Pol II transcription. In contrast, acute TBP depletion severely impairs RNA Polymerase III initiation. Furthermore, Pol II transcriptional induction occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to a functional redundancy with the TBP paralog TRF2, though TRF2 also binds to promoters of transcribed genes. Rather, we show that the TFIID complex can form and, despite having reduced TAF4 and TFIIA binding when TBP is depleted, the Pol II machinery is sufficiently robust in sustaining TBP-independent transcription. |
| first_indexed | 2024-04-09T13:18:25Z |
| format | Article |
| id | doaj.art-f3dbb978e91b4b0dbade89a695ea59df |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-09T13:18:25Z |
| publishDate | 2023-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f3dbb978e91b4b0dbade89a695ea59df2023-05-11T13:20:18ZengeLife Sciences Publications LtdeLife2050-084X2023-03-011210.7554/eLife.83810RNA Polymerase II transcription independent of TBP in murine embryonic stem cellsJames ZJ Kwan0https://orcid.org/0000-0003-4467-1749Thomas F Nguyen1https://orcid.org/0000-0003-3269-0846Anuli C Uzozie2Marek A Budzynski3Jieying Cui4Joseph MC Lee5Filip Van Petegem6Philipp F Lange7https://orcid.org/0000-0003-1171-5864Sheila S Teves8https://orcid.org/0000-0002-1220-2414Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada; Michael Cuccione Childhood Cancer Research Program, BC Children’s Hospital Research Institute, Vancouver, CanadaDepartment of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada; Michael Cuccione Childhood Cancer Research Program, BC Children’s Hospital Research Institute, Vancouver, CanadaDepartment of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, CanadaTranscription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation. Here, we report instead that acute depletion of TBP in mouse embryonic stem cells has no global effect on ongoing Pol II transcription. In contrast, acute TBP depletion severely impairs RNA Polymerase III initiation. Furthermore, Pol II transcriptional induction occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to a functional redundancy with the TBP paralog TRF2, though TRF2 also binds to promoters of transcribed genes. Rather, we show that the TFIID complex can form and, despite having reduced TAF4 and TFIIA binding when TBP is depleted, the Pol II machinery is sufficiently robust in sustaining TBP-independent transcription.https://elifesciences.org/articles/83810embryonic stem cellstranscriptiondegrongenomics |
| spellingShingle | James ZJ Kwan Thomas F Nguyen Anuli C Uzozie Marek A Budzynski Jieying Cui Joseph MC Lee Filip Van Petegem Philipp F Lange Sheila S Teves RNA Polymerase II transcription independent of TBP in murine embryonic stem cells eLife embryonic stem cells transcription degron genomics |
| title | RNA Polymerase II transcription independent of TBP in murine embryonic stem cells |
| title_full | RNA Polymerase II transcription independent of TBP in murine embryonic stem cells |
| title_fullStr | RNA Polymerase II transcription independent of TBP in murine embryonic stem cells |
| title_full_unstemmed | RNA Polymerase II transcription independent of TBP in murine embryonic stem cells |
| title_short | RNA Polymerase II transcription independent of TBP in murine embryonic stem cells |
| title_sort | rna polymerase ii transcription independent of tbp in murine embryonic stem cells |
| topic | embryonic stem cells transcription degron genomics |
| url | https://elifesciences.org/articles/83810 |
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