A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables
Imidacloprid (IMP) is toxic and a potential carcinogen that is most widely used as an insecticide for pest control and seed treatment. It is important to produce a rapid and sensitive assay for on-site monitoring. We have developed a novel lateral flow assay (LFA) using a sensitive monoclonal antibo...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/3/512 |
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| author | Steven Suryoprabowo Aihong Wu Liqiang Liu Hua Kuang Chuanlai Xu Lingling Guo |
| author_facet | Steven Suryoprabowo Aihong Wu Liqiang Liu Hua Kuang Chuanlai Xu Lingling Guo |
| author_sort | Steven Suryoprabowo |
| collection | DOAJ |
| description | Imidacloprid (IMP) is toxic and a potential carcinogen that is most widely used as an insecticide for pest control and seed treatment. It is important to produce a rapid and sensitive assay for on-site monitoring. We have developed a novel lateral flow assay (LFA) using a sensitive monoclonal antibody (mAb) for monitoring IMP residues on fruits and vegetables. The 50% inhibition concentration result that was found when using the ELISA method was 0.247 ng mL<sup>−1</sup>, with the cut-off limits using the LFA method the result was 10 ng mL<sup>−1</sup> (0.01 M PBS), and in the samples it was 20 ng mL<sup>−1</sup> (with a recovery rate of 96–104.7% for Chinese cabbage, cowpea, apple, and pear samples, respectively). All of the results can be determined within seven minutes. The proposed LFA method is a valid, quick, and stable assay for the on-site detection of IMP in large numbers of samples. |
| first_indexed | 2024-03-11T09:45:36Z |
| format | Article |
| id | doaj.art-f3dc2a8ee79d4496ace99b1d3e19c195 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T09:45:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-f3dc2a8ee79d4496ace99b1d3e19c1952023-11-16T16:40:25ZengMDPI AGFoods2304-81582023-01-0112351210.3390/foods12030512A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and VegetablesSteven Suryoprabowo0Aihong Wu1Liqiang Liu2Hua Kuang3Chuanlai Xu4Lingling Guo5State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaImidacloprid (IMP) is toxic and a potential carcinogen that is most widely used as an insecticide for pest control and seed treatment. It is important to produce a rapid and sensitive assay for on-site monitoring. We have developed a novel lateral flow assay (LFA) using a sensitive monoclonal antibody (mAb) for monitoring IMP residues on fruits and vegetables. The 50% inhibition concentration result that was found when using the ELISA method was 0.247 ng mL<sup>−1</sup>, with the cut-off limits using the LFA method the result was 10 ng mL<sup>−1</sup> (0.01 M PBS), and in the samples it was 20 ng mL<sup>−1</sup> (with a recovery rate of 96–104.7% for Chinese cabbage, cowpea, apple, and pear samples, respectively). All of the results can be determined within seven minutes. The proposed LFA method is a valid, quick, and stable assay for the on-site detection of IMP in large numbers of samples.https://www.mdpi.com/2304-8158/12/3/512gold immunochromatography testimidaclopridmonoclonal antibodystrip test |
| spellingShingle | Steven Suryoprabowo Aihong Wu Liqiang Liu Hua Kuang Chuanlai Xu Lingling Guo A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables Foods gold immunochromatography test imidacloprid monoclonal antibody strip test |
| title | A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables |
| title_full | A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables |
| title_fullStr | A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables |
| title_full_unstemmed | A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables |
| title_short | A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables |
| title_sort | rapid immunochromatographic method based on gold nanoparticles for the determination of imidacloprid on fruits and vegetables |
| topic | gold immunochromatography test imidacloprid monoclonal antibody strip test |
| url | https://www.mdpi.com/2304-8158/12/3/512 |
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