Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes
Introduction: Curcumin is a major phenolic compound of Curcuma longa, which has long been used in traditional Indian medicine. Recently, curcumin has been reported to have antihyperglycemic activity in animal models. However, the molecular basis of this action has not been adequatedly described. In...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Shahid Sadoughi University of Medical Sciences
2013-06-01
|
| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-285-1&slc_lang=en&sid=1 |
| _version_ | 1818379540330708992 |
|---|---|
| author | J Zavarreza F Pourrajab J Mohiti Ardakani Sh Asadi A Moradi |
| author_facet | J Zavarreza F Pourrajab J Mohiti Ardakani Sh Asadi A Moradi |
| author_sort | J Zavarreza |
| collection | DOAJ |
| description | Introduction: Curcumin is a major phenolic compound of Curcuma longa, which has long been used in traditional Indian medicine. Recently, curcumin has been reported to have antihyperglycemic activity in animal models. However, the molecular basis of this action has not been adequatedly described. In the present study the antihyperglycemic effect of curcumin was examined using C2C12 myoblast cells. Methods: The effects of curcumin were investigated in C2C12 myotubes by treating the cells with 40 µM of curcumin for 1.5 h. C2C12 myotubes were homogenized and the subcellular fractionation was prepared using ultracentrifugation; Then protein assay was performed using Bradford method and Glut4 determination was done using SDS-PAGE. Moreover, western immunoblotting techniques were exerted for semi-quantitative measurement. Data analysis was performed via gene tools software of Gel documentation and SPSS. An ANOVA test was used to compare three groups together. Results: Comparison of Glut4 levels in C2C12 myotubes showed that myotubes which were exposed to1.5 hours of 40 µM curcunin had higher Glut4 percentages in both cytosolic and membrane fractions and Glut4 percentages were significant with a confidence interval (CI) of 95% ( P<0.05 ). Conclusion: The study results showed that curcumin can strongly induce the increase of Glut4 translocation in differentiated C2C12 cells, indicating its possible regulatory role in the glucose metabolism of skeletal muscle cells |
| first_indexed | 2024-12-14T02:04:25Z |
| format | Article |
| id | doaj.art-f3dc9c1d8cbd4351a87bf9f687408745 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-14T02:04:25Z |
| publishDate | 2013-06-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-f3dc9c1d8cbd4351a87bf9f6874087452022-12-21T23:20:55ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332013-06-01212216225Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 MyotubesJ ZavarrezaF PourrajabJ Mohiti ArdakaniSh AsadiA MoradiIntroduction: Curcumin is a major phenolic compound of Curcuma longa, which has long been used in traditional Indian medicine. Recently, curcumin has been reported to have antihyperglycemic activity in animal models. However, the molecular basis of this action has not been adequatedly described. In the present study the antihyperglycemic effect of curcumin was examined using C2C12 myoblast cells. Methods: The effects of curcumin were investigated in C2C12 myotubes by treating the cells with 40 µM of curcumin for 1.5 h. C2C12 myotubes were homogenized and the subcellular fractionation was prepared using ultracentrifugation; Then protein assay was performed using Bradford method and Glut4 determination was done using SDS-PAGE. Moreover, western immunoblotting techniques were exerted for semi-quantitative measurement. Data analysis was performed via gene tools software of Gel documentation and SPSS. An ANOVA test was used to compare three groups together. Results: Comparison of Glut4 levels in C2C12 myotubes showed that myotubes which were exposed to1.5 hours of 40 µM curcunin had higher Glut4 percentages in both cytosolic and membrane fractions and Glut4 percentages were significant with a confidence interval (CI) of 95% ( P<0.05 ). Conclusion: The study results showed that curcumin can strongly induce the increase of Glut4 translocation in differentiated C2C12 cells, indicating its possible regulatory role in the glucose metabolism of skeletal muscle cellshttp://jssu.ssu.ac.ir/browse.php?a_code=A-10-285-1&slc_lang=en&sid=1CurcuminC2C12 myoblast cellsGlut4 |
| spellingShingle | J Zavarreza F Pourrajab J Mohiti Ardakani Sh Asadi A Moradi Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Curcumin C2C12 myoblast cells Glut4 |
| title | Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes |
| title_full | Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes |
| title_fullStr | Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes |
| title_full_unstemmed | Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes |
| title_short | Effect of curcumin Extract on Ttranslocation of Glut 4 in C2C12 Myotubes |
| title_sort | effect of curcumin extract on ttranslocation of glut 4 in c2c12 myotubes |
| topic | Curcumin C2C12 myoblast cells Glut4 |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-285-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT jzavarreza effectofcurcuminextractonttranslocationofglut4inc2c12myotubes AT fpourrajab effectofcurcuminextractonttranslocationofglut4inc2c12myotubes AT jmohitiardakani effectofcurcuminextractonttranslocationofglut4inc2c12myotubes AT shasadi effectofcurcuminextractonttranslocationofglut4inc2c12myotubes AT amoradi effectofcurcuminextractonttranslocationofglut4inc2c12myotubes |