Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses
Acyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of <i>Glycine soja</i><i>AAE3</i> (<i&g...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/22/8869 |
| _version_ | 1797547021032226816 |
|---|---|
| author | Peiqi Xian Zhandong Cai Yanbo Cheng Rongbin Lin Tengxiang Lian Qibin Ma Hai Nian |
| author_facet | Peiqi Xian Zhandong Cai Yanbo Cheng Rongbin Lin Tengxiang Lian Qibin Ma Hai Nian |
| author_sort | Peiqi Xian |
| collection | DOAJ |
| description | Acyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of <i>Glycine soja</i><i>AAE3</i> (<i>GsAAE3</i>) in Cadmium (Cd) and Aluminum (Al) tolerances. The recombinant GsAAE3 protein showed high activity toward oxalate, with a <i>K<sub>m</sub></i> of 105.10 ± 12.30 μM and <i>V<sub>max</sub></i> of 12.64 ± 0.34 μmol min<sup>−1</sup> mg<sup>−1</sup> protein, suggesting that it functions as an oxalyl–CoA synthetase. The expression of a GsAAE3–green fluorescent protein (GFP) fusion protein in tobacco leaves did not reveal a specific subcellular localization pattern of <i>GsAAE3</i>. An analysis of the <i>GsAAE3</i> expression pattern revealed an increase in <i>GsAAE3</i> expression in response to Cd and Al stresses, and it is mainly expressed in root tips. Furthermore, oxalate accumulation induced by Cd and Al contributes to the inhibition of root growth in wild soybean. Importantly, <i>GsAAE3</i> overexpression increases Cd and Al tolerances in <i>A. thaliana</i> and soybean hairy roots, which is associated with a decrease in oxalate accumulation. Taken together, our data provide evidence that the <i>GsAAE3</i>-encoded protein plays an important role in coping with Cd and Al stresses. |
| first_indexed | 2024-03-10T14:38:27Z |
| format | Article |
| id | doaj.art-f3dd701cea9840658f0218a24817fab3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:38:27Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f3dd701cea9840658f0218a24817fab32023-11-20T22:02:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122886910.3390/ijms21228869Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum StressesPeiqi Xian0Zhandong Cai1Yanbo Cheng2Rongbin Lin3Tengxiang Lian4Qibin Ma5Hai Nian6The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaThe State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaThe State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaThe State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaThe State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaThe State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaThe State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaAcyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of <i>Glycine soja</i><i>AAE3</i> (<i>GsAAE3</i>) in Cadmium (Cd) and Aluminum (Al) tolerances. The recombinant GsAAE3 protein showed high activity toward oxalate, with a <i>K<sub>m</sub></i> of 105.10 ± 12.30 μM and <i>V<sub>max</sub></i> of 12.64 ± 0.34 μmol min<sup>−1</sup> mg<sup>−1</sup> protein, suggesting that it functions as an oxalyl–CoA synthetase. The expression of a GsAAE3–green fluorescent protein (GFP) fusion protein in tobacco leaves did not reveal a specific subcellular localization pattern of <i>GsAAE3</i>. An analysis of the <i>GsAAE3</i> expression pattern revealed an increase in <i>GsAAE3</i> expression in response to Cd and Al stresses, and it is mainly expressed in root tips. Furthermore, oxalate accumulation induced by Cd and Al contributes to the inhibition of root growth in wild soybean. Importantly, <i>GsAAE3</i> overexpression increases Cd and Al tolerances in <i>A. thaliana</i> and soybean hairy roots, which is associated with a decrease in oxalate accumulation. Taken together, our data provide evidence that the <i>GsAAE3</i>-encoded protein plays an important role in coping with Cd and Al stresses.https://www.mdpi.com/1422-0067/21/22/8869acyl activating enzyme 3oxalyl-CoA synthetaseoxalate accumulationCd and Al tolerancewild soybean |
| spellingShingle | Peiqi Xian Zhandong Cai Yanbo Cheng Rongbin Lin Tengxiang Lian Qibin Ma Hai Nian Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses International Journal of Molecular Sciences acyl activating enzyme 3 oxalyl-CoA synthetase oxalate accumulation Cd and Al tolerance wild soybean |
| title | Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses |
| title_full | Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses |
| title_fullStr | Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses |
| title_full_unstemmed | Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses |
| title_short | Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses |
| title_sort | wild soybean oxalyl coa synthetase degrades oxalate and affects the tolerance to cadmium and aluminum stresses |
| topic | acyl activating enzyme 3 oxalyl-CoA synthetase oxalate accumulation Cd and Al tolerance wild soybean |
| url | https://www.mdpi.com/1422-0067/21/22/8869 |
| work_keys_str_mv | AT peiqixian wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses AT zhandongcai wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses AT yanbocheng wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses AT rongbinlin wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses AT tengxianglian wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses AT qibinma wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses AT hainian wildsoybeanoxalylcoasynthetasedegradesoxalateandaffectsthetolerancetocadmiumandaluminumstresses |