Identification and characterization of suppressor mutants of stop1
Abstract Background Proton stress and aluminum (Al) toxicity are major constraints limiting crop growth and yields on acid soils (pH < 5). In Arabidopsis, STOP1 is a master transcription factor that controls the expression of a set of well-characterized Al tolerance genes and unknown processes in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-017-1079-2 |
| _version_ | 1818096908165447680 |
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| author | Fei Jiang Tao Wang Yuqi Wang Leon V. Kochian Fang Chen Jiping Liu |
| author_facet | Fei Jiang Tao Wang Yuqi Wang Leon V. Kochian Fang Chen Jiping Liu |
| author_sort | Fei Jiang |
| collection | DOAJ |
| description | Abstract Background Proton stress and aluminum (Al) toxicity are major constraints limiting crop growth and yields on acid soils (pH < 5). In Arabidopsis, STOP1 is a master transcription factor that controls the expression of a set of well-characterized Al tolerance genes and unknown processes involved in low pH resistance. As a result, loss-of-function stop1 mutants are extremely sensitive to low pH and Al stresses. Results Here, we report on screens of an ethyl-methane sulphonate (EMS)-mutagenized stop1 population and isolation of nine strong stop1 suppressor mutants, i.e., the tolerant to proton stress (tps) mutants, with significantly enhanced root growth at low pH (4.3). Genetic analyses indicated these dominant and partial gain-of-function mutants are caused by mutations in single nuclear genes outside the STOP1 locus. Physiological characterization of the responses of these tps mutants to excess levels of Al and other metal ions further classified them into five groups. Three tps mutants also displayed enhanced resistance to Al stress, indicating that these tps mutations partially rescue the hypersensitive phenotypes of stop1 to both low pH stress and Al stress. The other six tps mutants showed enhanced resistance only to low pH stress but not to Al stress. We carried out further physiologic and mapping-by-sequencing analyses for two tps mutants with enhanced resistance to both low pH and Al stresses and identified the genomic regions and candidate loci in chromosomes 1 and 2 that harbor these two TPS genes. Conclusion We have identified and characterized nine strong stop1 suppressor mutants. Candidate loci for two tps mutations that partially rescue the hypersensitive phenotypes of stop1 to low pH and Al stresses were identified by mapping-by-sequencing approaches. Further studies could provide insights into the structure and function of TPSs and the regulatory networks underlying the STOP1-mediated processes that lead to resistance to low pH and Al stresses in Arabidopsis. |
| first_indexed | 2024-12-10T23:12:06Z |
| format | Article |
| id | doaj.art-f3de0beb0aa04d949fc3f3ac2d47211c |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-10T23:12:06Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-f3de0beb0aa04d949fc3f3ac2d47211c2022-12-22T01:29:56ZengBMCBMC Plant Biology1471-22292017-07-0117111010.1186/s12870-017-1079-2Identification and characterization of suppressor mutants of stop1Fei Jiang0Tao Wang1Yuqi Wang2Leon V. Kochian3Fang Chen4Jiping Liu5Robert W. Holley Center, US Department of Agriculture-Agricultural Research ServiceChengdu Institute of Biology, Chinese Academy of SciencesRobert W. Holley Center, US Department of Agriculture-Agricultural Research ServiceGlobal Institute for Food Security, University of SaskatchewanCollege of Life Science, Sichuan UniversityRobert W. Holley Center, US Department of Agriculture-Agricultural Research ServiceAbstract Background Proton stress and aluminum (Al) toxicity are major constraints limiting crop growth and yields on acid soils (pH < 5). In Arabidopsis, STOP1 is a master transcription factor that controls the expression of a set of well-characterized Al tolerance genes and unknown processes involved in low pH resistance. As a result, loss-of-function stop1 mutants are extremely sensitive to low pH and Al stresses. Results Here, we report on screens of an ethyl-methane sulphonate (EMS)-mutagenized stop1 population and isolation of nine strong stop1 suppressor mutants, i.e., the tolerant to proton stress (tps) mutants, with significantly enhanced root growth at low pH (4.3). Genetic analyses indicated these dominant and partial gain-of-function mutants are caused by mutations in single nuclear genes outside the STOP1 locus. Physiological characterization of the responses of these tps mutants to excess levels of Al and other metal ions further classified them into five groups. Three tps mutants also displayed enhanced resistance to Al stress, indicating that these tps mutations partially rescue the hypersensitive phenotypes of stop1 to both low pH stress and Al stress. The other six tps mutants showed enhanced resistance only to low pH stress but not to Al stress. We carried out further physiologic and mapping-by-sequencing analyses for two tps mutants with enhanced resistance to both low pH and Al stresses and identified the genomic regions and candidate loci in chromosomes 1 and 2 that harbor these two TPS genes. Conclusion We have identified and characterized nine strong stop1 suppressor mutants. Candidate loci for two tps mutations that partially rescue the hypersensitive phenotypes of stop1 to low pH and Al stresses were identified by mapping-by-sequencing approaches. Further studies could provide insights into the structure and function of TPSs and the regulatory networks underlying the STOP1-mediated processes that lead to resistance to low pH and Al stresses in Arabidopsis.http://link.springer.com/article/10.1186/s12870-017-1079-2ALMT1Aluminum toxicityMATEProton toxicitySTOP1Suppressor mutants |
| spellingShingle | Fei Jiang Tao Wang Yuqi Wang Leon V. Kochian Fang Chen Jiping Liu Identification and characterization of suppressor mutants of stop1 BMC Plant Biology ALMT1 Aluminum toxicity MATE Proton toxicity STOP1 Suppressor mutants |
| title | Identification and characterization of suppressor mutants of stop1 |
| title_full | Identification and characterization of suppressor mutants of stop1 |
| title_fullStr | Identification and characterization of suppressor mutants of stop1 |
| title_full_unstemmed | Identification and characterization of suppressor mutants of stop1 |
| title_short | Identification and characterization of suppressor mutants of stop1 |
| title_sort | identification and characterization of suppressor mutants of stop1 |
| topic | ALMT1 Aluminum toxicity MATE Proton toxicity STOP1 Suppressor mutants |
| url | http://link.springer.com/article/10.1186/s12870-017-1079-2 |
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