Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging.
After percutaneous implantation of a cardiac occluder, a complex healing process leads to the device coverage within several months. An incomplete device coverage increases the risk of device related complications such as thrombosis or endocarditis. We aimed to assess the device coverage process of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0284471 |
| _version_ | 1797829999631269888 |
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| author | Elodie Perdreau Zakaria Jalal Richard D Walton Matthias Sigler Hubert Cochet Jérôme Naulin Bruno Quesson Olivier Bernus Jean-Benoît Thambo |
| author_facet | Elodie Perdreau Zakaria Jalal Richard D Walton Matthias Sigler Hubert Cochet Jérôme Naulin Bruno Quesson Olivier Bernus Jean-Benoît Thambo |
| author_sort | Elodie Perdreau |
| collection | DOAJ |
| description | After percutaneous implantation of a cardiac occluder, a complex healing process leads to the device coverage within several months. An incomplete device coverage increases the risk of device related complications such as thrombosis or endocarditis. We aimed to assess the device coverage process of atrial septal defect (ASD) occluders in a chronic sheep model using micro-computed tomography (micro-CT). After percutaneous creation of an ASD, 8 ewes were implanted with a 16-mm Nit-Occlud ASD-R occluder (PFM medical, Cologne, Germany) and were followed for 1 month (N = 3) and 3 months (N = 5). After heart explant, the device coverage was assessed using micro-CT (resolution of 41.7 μm) and was compared to histological analysis. The micro-CT image reconstruction was performed in 2D and 3D allowing measurement of the coverage thickness and surface for each device. Macroscopic assessment of devices showed that the coverage was complete for the left-side disk in all cases. Yet incomplete coverage of the right-side disk was observed in 5 of the 8 cases. 2D and 3D micro-CT analysis allowed an accurate evaluation of device coverage of each disk and was overall well correlated to histology sections. Surface calculation from micro-CT images of the 8 cases showed that the median surface of coverage was 93±8% for the left-side disk and 55±31% for the right-side disk. The assessment of tissue reactions, including endothelialisation, after implantation of an ASD occluder can rely on in vitro micro-CT analysis. The translation to clinical practice is challenging but the potential for individual follow-up is shown, to avoid thrombotic or infective complications. |
| first_indexed | 2024-04-09T13:29:07Z |
| format | Article |
| id | doaj.art-f3e3625685a4467aa95e521ba589d097 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T13:29:07Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f3e3625685a4467aa95e521ba589d0972023-05-10T05:31:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01184e028447110.1371/journal.pone.0284471Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging.Elodie PerdreauZakaria JalalRichard D WaltonMatthias SiglerHubert CochetJérôme NaulinBruno QuessonOlivier BernusJean-Benoît ThamboAfter percutaneous implantation of a cardiac occluder, a complex healing process leads to the device coverage within several months. An incomplete device coverage increases the risk of device related complications such as thrombosis or endocarditis. We aimed to assess the device coverage process of atrial septal defect (ASD) occluders in a chronic sheep model using micro-computed tomography (micro-CT). After percutaneous creation of an ASD, 8 ewes were implanted with a 16-mm Nit-Occlud ASD-R occluder (PFM medical, Cologne, Germany) and were followed for 1 month (N = 3) and 3 months (N = 5). After heart explant, the device coverage was assessed using micro-CT (resolution of 41.7 μm) and was compared to histological analysis. The micro-CT image reconstruction was performed in 2D and 3D allowing measurement of the coverage thickness and surface for each device. Macroscopic assessment of devices showed that the coverage was complete for the left-side disk in all cases. Yet incomplete coverage of the right-side disk was observed in 5 of the 8 cases. 2D and 3D micro-CT analysis allowed an accurate evaluation of device coverage of each disk and was overall well correlated to histology sections. Surface calculation from micro-CT images of the 8 cases showed that the median surface of coverage was 93±8% for the left-side disk and 55±31% for the right-side disk. The assessment of tissue reactions, including endothelialisation, after implantation of an ASD occluder can rely on in vitro micro-CT analysis. The translation to clinical practice is challenging but the potential for individual follow-up is shown, to avoid thrombotic or infective complications.https://doi.org/10.1371/journal.pone.0284471 |
| spellingShingle | Elodie Perdreau Zakaria Jalal Richard D Walton Matthias Sigler Hubert Cochet Jérôme Naulin Bruno Quesson Olivier Bernus Jean-Benoît Thambo Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging. PLoS ONE |
| title | Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging. |
| title_full | Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging. |
| title_fullStr | Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging. |
| title_full_unstemmed | Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging. |
| title_short | Assessment of Nit-Occlud atrial septal defect occluder device healing process using micro-computed tomography imaging. |
| title_sort | assessment of nit occlud atrial septal defect occluder device healing process using micro computed tomography imaging |
| url | https://doi.org/10.1371/journal.pone.0284471 |
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