Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i>
The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the <i>sulA</i> gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations....
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4535 |
| _version_ | 1797536216613126144 |
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| author | Masayuki Murata Keiko Nakamura Tomoyuki Kosaka Natsuko Ota Ayumi Osawa Ryunosuke Muro Kazuya Fujiyama Taku Oshima Hirotada Mori Barry L. Wanner Mamoru Yamada |
| author_facet | Masayuki Murata Keiko Nakamura Tomoyuki Kosaka Natsuko Ota Ayumi Osawa Ryunosuke Muro Kazuya Fujiyama Taku Oshima Hirotada Mori Barry L. Wanner Mamoru Yamada |
| author_sort | Masayuki Murata |
| collection | DOAJ |
| description | The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the <i>sulA</i> gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations. Overexpression of <i>sulA</i> leads to cell lysis, suggesting SulA eliminates cells with unrepaired damaged DNA. Transcriptome analysis revealed that overexpression of <i>sulA</i> leads to up-regulation of numerous genes, including <i>soxS.</i> Deletion of <i>soxS</i> markedly reduced the extent of cell lysis by <i>sulA</i> overexpression and <i>soxS</i> overexpression alone led to cell lysis. Further experiments on the SoxS regulon suggested that LpxC is a main player downstream from SoxS. These findings suggested the SulA-dependent cell lysis (SDCL) cascade as follows: SulA→SoxS→LpxC. Other tests showed that the SDCL cascade pathway does not overlap with the apoptosis-like and <i>mazEF</i> cell death pathways. |
| first_indexed | 2024-03-10T11:57:29Z |
| format | Article |
| id | doaj.art-f3e916f9d9a14681a02446dbcd915441 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:57:29Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f3e916f9d9a14681a02446dbcd9154412023-11-21T17:15:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01229453510.3390/ijms22094535Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i>Masayuki Murata0Keiko Nakamura1Tomoyuki Kosaka2Natsuko Ota3Ayumi Osawa4Ryunosuke Muro5Kazuya Fujiyama6Taku Oshima7Hirotada Mori8Barry L. Wanner9Mamoru Yamada10Life Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube 755-8611, JapanApplied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Ube 755-8505, JapanLife Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube 755-8611, JapanLife Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube 755-8611, JapanLife Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube 755-8611, JapanApplied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Ube 755-8505, JapanApplied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Ube 755-8505, JapanDepartment of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, JapanGraduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, JapanDepartment of Microbiology, Harvard Medical School, Boston, MA 02115, USALife Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube 755-8611, JapanThe SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the <i>sulA</i> gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations. Overexpression of <i>sulA</i> leads to cell lysis, suggesting SulA eliminates cells with unrepaired damaged DNA. Transcriptome analysis revealed that overexpression of <i>sulA</i> leads to up-regulation of numerous genes, including <i>soxS.</i> Deletion of <i>soxS</i> markedly reduced the extent of cell lysis by <i>sulA</i> overexpression and <i>soxS</i> overexpression alone led to cell lysis. Further experiments on the SoxS regulon suggested that LpxC is a main player downstream from SoxS. These findings suggested the SulA-dependent cell lysis (SDCL) cascade as follows: SulA→SoxS→LpxC. Other tests showed that the SDCL cascade pathway does not overlap with the apoptosis-like and <i>mazEF</i> cell death pathways.https://www.mdpi.com/1422-0067/22/9/4535DNA damageSulAcell lysisSoxSLpxC |
| spellingShingle | Masayuki Murata Keiko Nakamura Tomoyuki Kosaka Natsuko Ota Ayumi Osawa Ryunosuke Muro Kazuya Fujiyama Taku Oshima Hirotada Mori Barry L. Wanner Mamoru Yamada Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i> International Journal of Molecular Sciences DNA damage SulA cell lysis SoxS LpxC |
| title | Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i> |
| title_full | Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i> |
| title_fullStr | Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i> |
| title_full_unstemmed | Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i> |
| title_short | Cell Lysis Directed by SulA in Response to DNA Damage in <i>Escherichia coli</i> |
| title_sort | cell lysis directed by sula in response to dna damage in i escherichia coli i |
| topic | DNA damage SulA cell lysis SoxS LpxC |
| url | https://www.mdpi.com/1422-0067/22/9/4535 |
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