Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina)
In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to <i>Ale...
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MDPI AG
2023-02-01
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| Series: | Phycology |
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| Online Access: | https://www.mdpi.com/2673-9410/3/1/6 |
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| author | Jonás Adrián Tardivo Kubis Francisco Rodríguez Araceli E. Rossignoli Pilar Riobó Eugenia A. Sar Inés Sunesen |
| author_facet | Jonás Adrián Tardivo Kubis Francisco Rodríguez Araceli E. Rossignoli Pilar Riobó Eugenia A. Sar Inés Sunesen |
| author_sort | Jonás Adrián Tardivo Kubis |
| collection | DOAJ |
| description | In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to <i>Alexandrium catenella</i>, <i>Protoceratium reticulatum</i> and <i>Pseudo-nitzschia multiseries</i>. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of <i>A. catenella</i>, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell<sup>−1</sup>). The toxin profile of <i>P. reticulatum</i> was dominated by yessotoxins (YTXs) (up to 94.40 pg cell<sup>−1</sup>) accompanied by homo-yessotoxin (Homo-YTX) traces. In <i>P. multiseries</i>, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell<sup>−1</sup> and 1.09 pg cell<sup>−1</sup>) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms. |
| first_indexed | 2024-03-11T06:01:45Z |
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| institution | Directory Open Access Journal |
| issn | 2673-9410 |
| language | English |
| last_indexed | 2024-03-11T06:01:45Z |
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| spelling | doaj.art-f3ed5363b5894f5d93826f6be7fb92522023-11-17T13:19:48ZengMDPI AGPhycology2673-94102023-02-01317910510.3390/phycology3010006Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina)Jonás Adrián Tardivo Kubis0Francisco Rodríguez1Araceli E. Rossignoli2Pilar Riobó3Eugenia A. Sar4Inés Sunesen5División Ficología Dr. Sebastián Guarrera, FCNyM, Paseo del Bosque s/n, La Plata 1900, ArgentinaDepartamento de Microalgas Nocivas, Instituto Español de Oceanografía (IEO-CSIC), Subida a Radio Faro 50, 36390 Vigo, SpainProcesos Oceanográficos Costeiros, Centro de Investigacións Mariñas (CIMA), Pedras de Corón s/n, 36620 Vilanova de Arousa, SpainDepartamento de Fotobiología y Toxinología de Fitoplancton, Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello 6, 36208 Vigo, SpainDivisión Ficología Dr. Sebastián Guarrera, FCNyM, Paseo del Bosque s/n, La Plata 1900, ArgentinaDivisión Ficología Dr. Sebastián Guarrera, FCNyM, Paseo del Bosque s/n, La Plata 1900, ArgentinaIn the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to <i>Alexandrium catenella</i>, <i>Protoceratium reticulatum</i> and <i>Pseudo-nitzschia multiseries</i>. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of <i>A. catenella</i>, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell<sup>−1</sup>). The toxin profile of <i>P. reticulatum</i> was dominated by yessotoxins (YTXs) (up to 94.40 pg cell<sup>−1</sup>) accompanied by homo-yessotoxin (Homo-YTX) traces. In <i>P. multiseries</i>, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell<sup>−1</sup> and 1.09 pg cell<sup>−1</sup>) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.https://www.mdpi.com/2673-9410/3/1/6<i>Alexandrium catenella</i>molecular analysismorphology<i>Protoceratium reticulatum</i><i>Pseudo-nitzschia multiseries</i>toxinological profiles |
| spellingShingle | Jonás Adrián Tardivo Kubis Francisco Rodríguez Araceli E. Rossignoli Pilar Riobó Eugenia A. Sar Inés Sunesen Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina) Phycology <i>Alexandrium catenella</i> molecular analysis morphology <i>Protoceratium reticulatum</i> <i>Pseudo-nitzschia multiseries</i> toxinological profiles |
| title | Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina) |
| title_full | Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina) |
| title_fullStr | Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina) |
| title_full_unstemmed | Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina) |
| title_short | Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina) |
| title_sort | morphological phylogenetic and toxinological characterization of potentially harmful algal species from the marine coastal waters of buenos aires province argentina |
| topic | <i>Alexandrium catenella</i> molecular analysis morphology <i>Protoceratium reticulatum</i> <i>Pseudo-nitzschia multiseries</i> toxinological profiles |
| url | https://www.mdpi.com/2673-9410/3/1/6 |
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