Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History
Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-02-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/12/2/1544/ |
| _version_ | 1798003458401370112 |
|---|---|
| author | Gary S. Sayler Gabriela Kuncová Josef Trögl Steven Ripp Alice C. Layton Archana Chauhan |
| author_facet | Gary S. Sayler Gabriela Kuncová Josef Trögl Steven Ripp Alice C. Layton Archana Chauhan |
| author_sort | Gary S. Sayler |
| collection | DOAJ |
| description | Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution. |
| first_indexed | 2024-04-11T12:07:57Z |
| format | Article |
| id | doaj.art-f3f0e44410b448408685db8f2ab211cc |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:07:57Z |
| publishDate | 2012-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-f3f0e44410b448408685db8f2ab211cc2022-12-22T04:24:40ZengMDPI AGSensors1424-82202012-02-011221544157110.3390/s120201544Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application HistoryGary S. SaylerGabriela KuncováJosef TröglSteven RippAlice C. LaytonArchana ChauhanInitially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution.http://www.mdpi.com/1424-8220/12/2/1544/bioluminescencebioreporterbiosensorsPseudomonas fluorescens HK44lux genes |
| spellingShingle | Gary S. Sayler Gabriela Kuncová Josef Trögl Steven Ripp Alice C. Layton Archana Chauhan Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History Sensors bioluminescence bioreporter biosensors Pseudomonas fluorescens HK44 lux genes |
| title | Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History |
| title_full | Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History |
| title_fullStr | Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History |
| title_full_unstemmed | Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History |
| title_short | Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History |
| title_sort | pseudomonas fluorescens hk44 lessons learned from a model whole cell bioreporter with a broad application history |
| topic | bioluminescence bioreporter biosensors Pseudomonas fluorescens HK44 lux genes |
| url | http://www.mdpi.com/1424-8220/12/2/1544/ |
| work_keys_str_mv | AT garyssayler pseudomonasfluorescenshk44lessonslearnedfromamodelwholecellbioreporterwithabroadapplicationhistory AT gabrielakuncova pseudomonasfluorescenshk44lessonslearnedfromamodelwholecellbioreporterwithabroadapplicationhistory AT joseftrogl pseudomonasfluorescenshk44lessonslearnedfromamodelwholecellbioreporterwithabroadapplicationhistory AT stevenripp pseudomonasfluorescenshk44lessonslearnedfromamodelwholecellbioreporterwithabroadapplicationhistory AT aliceclayton pseudomonasfluorescenshk44lessonslearnedfromamodelwholecellbioreporterwithabroadapplicationhistory AT archanachauhan pseudomonasfluorescenshk44lessonslearnedfromamodelwholecellbioreporterwithabroadapplicationhistory |