Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were asse...
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| Format: | Article |
| Language: | English |
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BMC
2007-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004 |
| _version_ | 1811204761235488768 |
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| author | HOSSEIN MOZDARANI SHABNAM Z MORADI |
| author_facet | HOSSEIN MOZDARANI SHABNAM Z MORADI |
| author_sort | HOSSEIN MOZDARANI |
| collection | DOAJ |
| description | This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration (24 hours, 1 and 2 weeks, 1-6 months) were frozen. After appropriate storage periods embryos were thawed and studied for their viability 4-6 hours after thawing and intact embryos were transferred to fresh medium containing colcemid. After 48 hours, the embryos were fixed and studied for their chromosome abnormalities using Tarkowsky's drying technique. Results indicate that freezing affects the viability and chromosome structure of embryos when compared with the controligroup. Furthermore increasing the storage duration reduces the viability and increases the chromosome aberrations of embryos (such as aneuploidy and polyploidy). This result mµght indicate that the effects of vitrification on the cytoskeleton or other cellular organelle mµght produce chromosomal alterations leading to cell death |
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| format | Article |
| id | doaj.art-f3f2a2aab05b49caa6c74caedb88ce35 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-04-12T03:19:10Z |
| publishDate | 2007-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-f3f2a2aab05b49caa6c74caedb88ce352022-12-22T03:49:59ZengBMCBiological Research0716-97600717-62872007-01-01403299306Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durationsHOSSEIN MOZDARANISHABNAM Z MORADIThis study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration (24 hours, 1 and 2 weeks, 1-6 months) were frozen. After appropriate storage periods embryos were thawed and studied for their viability 4-6 hours after thawing and intact embryos were transferred to fresh medium containing colcemid. After 48 hours, the embryos were fixed and studied for their chromosome abnormalities using Tarkowsky's drying technique. Results indicate that freezing affects the viability and chromosome structure of embryos when compared with the controligroup. Furthermore increasing the storage duration reduces the viability and increases the chromosome aberrations of embryos (such as aneuploidy and polyploidy). This result mµght indicate that the effects of vitrification on the cytoskeleton or other cellular organelle mµght produce chromosomal alterations leading to cell deathhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004Cryopreservationvitrificationmouse embryoviabilitychromosome abnormalities |
| spellingShingle | HOSSEIN MOZDARANI SHABNAM Z MORADI Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations Biological Research Cryopreservation vitrification mouse embryo viability chromosome abnormalities |
| title | Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations |
| title_full | Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations |
| title_fullStr | Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations |
| title_full_unstemmed | Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations |
| title_short | Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations |
| title_sort | effect of vitrification on viability and chromosome abnormalities in 8 cell mouse embryos at various storage durations |
| topic | Cryopreservation vitrification mouse embryo viability chromosome abnormalities |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004 |
| work_keys_str_mv | AT hosseinmozdarani effectofvitrificationonviabilityandchromosomeabnormalitiesin8cellmouseembryosatvariousstoragedurations AT shabnamzmoradi effectofvitrificationonviabilityandchromosomeabnormalitiesin8cellmouseembryosatvariousstoragedurations |