An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function
Neuropeptides are released by neurons that are involved in a wide range of brain functions, such as food intake, metabolism, reproduction, and learning and memory. A full-length cDNA sequence of an <i>FMRFamide</i> gene isolated from the cuttlefish <i>Sepia pharaonis</i> (des...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/7/1636 |
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| author | Yang Zhu Lian-lian Sun Jun-hong Wu Hui-hui Liu Li-bing Zheng Zhen-ming Lü Chang-feng Chi |
| author_facet | Yang Zhu Lian-lian Sun Jun-hong Wu Hui-hui Liu Li-bing Zheng Zhen-ming Lü Chang-feng Chi |
| author_sort | Yang Zhu |
| collection | DOAJ |
| description | Neuropeptides are released by neurons that are involved in a wide range of brain functions, such as food intake, metabolism, reproduction, and learning and memory. A full-length cDNA sequence of an <i>FMRFamide</i> gene isolated from the cuttlefish <i>Sepia pharaonis</i> (designated as <i>SpFMRFamide</i>) was cloned. The predicted precursor protein contains one putative signal peptide and four FMRFamide-related peptides. Multiple amino acid and nucleotide sequence alignments showed that it shares 97% similarity with the precursor FMRFamides of <i>Sepiella japonica</i> and <i>Sepia officinalis</i> and shares 93% and 92% similarity with the <i>SpFMRFamide</i> gene of the two cuttlefish species, respectively. Moreover, the phylogenetic analysis also suggested that <i>Sp</i>FMRFamide and FMRFamides from <i>S. japonica</i> and <i>S. officinalis</i> belong to the same sub-branch. Tissue expression analysis confirmed that <i>SpFMRFamide</i> was widely distributed among tissues and predominantly expressed in the brain at the three development stages. The combined effects of <i>Sp</i>FMRFamide+<i>Sp</i>GnRH and <i>Sp</i>FLRFamide+<i>Sp</i>GnRH showed a marked decrease in the level of the total proteins released in the CHO-K1 cells. This is the first report of <i>SpFMRFamide</i> in <i>S. pharaonis</i> and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T20:44:26Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f3f32ca9becb46da8ca695d8171bced32023-11-19T20:29:22ZengMDPI AGMolecules1420-30492020-04-01257163610.3390/molecules25071636An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential FunctionYang Zhu0Lian-lian Sun1Jun-hong Wu2Hui-hui Liu3Li-bing Zheng4Zhen-ming Lü5Chang-feng Chi6National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan 316022, ChinaNeuropeptides are released by neurons that are involved in a wide range of brain functions, such as food intake, metabolism, reproduction, and learning and memory. A full-length cDNA sequence of an <i>FMRFamide</i> gene isolated from the cuttlefish <i>Sepia pharaonis</i> (designated as <i>SpFMRFamide</i>) was cloned. The predicted precursor protein contains one putative signal peptide and four FMRFamide-related peptides. Multiple amino acid and nucleotide sequence alignments showed that it shares 97% similarity with the precursor FMRFamides of <i>Sepiella japonica</i> and <i>Sepia officinalis</i> and shares 93% and 92% similarity with the <i>SpFMRFamide</i> gene of the two cuttlefish species, respectively. Moreover, the phylogenetic analysis also suggested that <i>Sp</i>FMRFamide and FMRFamides from <i>S. japonica</i> and <i>S. officinalis</i> belong to the same sub-branch. Tissue expression analysis confirmed that <i>SpFMRFamide</i> was widely distributed among tissues and predominantly expressed in the brain at the three development stages. The combined effects of <i>Sp</i>FMRFamide+<i>Sp</i>GnRH and <i>Sp</i>FLRFamide+<i>Sp</i>GnRH showed a marked decrease in the level of the total proteins released in the CHO-K1 cells. This is the first report of <i>SpFMRFamide</i> in <i>S. pharaonis</i> and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species.https://www.mdpi.com/1420-3049/25/7/1636neuropeptideFMRFamide<i>Sepia pharaonis</i>cuttlefish |
| spellingShingle | Yang Zhu Lian-lian Sun Jun-hong Wu Hui-hui Liu Li-bing Zheng Zhen-ming Lü Chang-feng Chi An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function Molecules neuropeptide FMRFamide <i>Sepia pharaonis</i> cuttlefish |
| title | An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function |
| title_full | An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function |
| title_fullStr | An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function |
| title_full_unstemmed | An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function |
| title_short | An FMRFamide Neuropeptide in Cuttlefish <i>Sepia pharaonis</i>: Identification, Characterization, and Potential Function |
| title_sort | fmrfamide neuropeptide in cuttlefish i sepia pharaonis i identification characterization and potential function |
| topic | neuropeptide FMRFamide <i>Sepia pharaonis</i> cuttlefish |
| url | https://www.mdpi.com/1420-3049/25/7/1636 |
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