Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer
Background:The diagnosis of Precancerous Lesions of Gastric Cancer (PLGC) is challenging in clinical practice. We conducted a clinical study by analyzing the information of relevant chromosome copy number variations (CNV) in the TCGA database followed by the UCAD technique to evaluate the value of C...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1359231/full |
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| author | Suting Qian Feifei Xie Haoyu Zhao Ting Jiang Yi Sang Wei Ye Qingsheng Liu Danli Cai |
| author_facet | Suting Qian Feifei Xie Haoyu Zhao Ting Jiang Yi Sang Wei Ye Qingsheng Liu Danli Cai |
| author_sort | Suting Qian |
| collection | DOAJ |
| description | Background:The diagnosis of Precancerous Lesions of Gastric Cancer (PLGC) is challenging in clinical practice. We conducted a clinical study by analyzing the information of relevant chromosome copy number variations (CNV) in the TCGA database followed by the UCAD technique to evaluate the value of Chromosomal Instability (CIN) assay in the diagnosis of PLGC.Methods:Based on the screening of gastric cancer related data in TCGA database, CNV analysis was performed to explore the information of chromosome CNV related to gastric cancer. Based on the gastroscopic pathology results, 12 specimens of patients with severe atrophy were screened to analyze the paraffin specimens of gastric mucosa by UCAD technology, and to explore the influence of related factors on them.Results:The results of CNV in TCGA database suggested that chromosome 7, 8, and 17 amplification was obvious in patients with gastric cancer. UCAD results confirmed that in 12 patients with pathologic diagnosis of severe atrophy, five of them had positive results of CIN, with a positive detection rate of 41.7%, which was mainly manifested in chromosome seven and chromosome eight segments amplification. We also found that intestinalization and HP infection were less associated with CIN. And the sensitivity of CIN measurement results was significantly better than that of tumor indicators.Conclusion:The findings suggest that the diagnosis of PLGC can be aided by UCAD detection of CIN, of which Chr7 and 8 may be closely related to PLGC. |
| first_indexed | 2024-04-24T11:33:39Z |
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| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-24T11:33:39Z |
| publishDate | 2024-04-01 |
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| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-f3f46b882e5f48f7bf279832b691b5a02024-04-10T08:03:00ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-04-011510.3389/fgene.2024.13592311359231Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancerSuting Qian0Feifei Xie1Haoyu Zhao2Ting Jiang3Yi Sang4Wei Ye5Qingsheng Liu6Danli Cai7Hangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaHangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaHangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaHangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaHangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaHangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaHangzhou Hospital of TCM Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaIntensive Care Unit, The First Affiliated hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, ChinaBackground:The diagnosis of Precancerous Lesions of Gastric Cancer (PLGC) is challenging in clinical practice. We conducted a clinical study by analyzing the information of relevant chromosome copy number variations (CNV) in the TCGA database followed by the UCAD technique to evaluate the value of Chromosomal Instability (CIN) assay in the diagnosis of PLGC.Methods:Based on the screening of gastric cancer related data in TCGA database, CNV analysis was performed to explore the information of chromosome CNV related to gastric cancer. Based on the gastroscopic pathology results, 12 specimens of patients with severe atrophy were screened to analyze the paraffin specimens of gastric mucosa by UCAD technology, and to explore the influence of related factors on them.Results:The results of CNV in TCGA database suggested that chromosome 7, 8, and 17 amplification was obvious in patients with gastric cancer. UCAD results confirmed that in 12 patients with pathologic diagnosis of severe atrophy, five of them had positive results of CIN, with a positive detection rate of 41.7%, which was mainly manifested in chromosome seven and chromosome eight segments amplification. We also found that intestinalization and HP infection were less associated with CIN. And the sensitivity of CIN measurement results was significantly better than that of tumor indicators.Conclusion:The findings suggest that the diagnosis of PLGC can be aided by UCAD detection of CIN, of which Chr7 and 8 may be closely related to PLGC.https://www.frontiersin.org/articles/10.3389/fgene.2024.1359231/fullultrasensitive chromosomal aneuploidy detectionchromosome instabilitycopy number variationsprecancerous lesions of gastric cancergene |
| spellingShingle | Suting Qian Feifei Xie Haoyu Zhao Ting Jiang Yi Sang Wei Ye Qingsheng Liu Danli Cai Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer Frontiers in Genetics ultrasensitive chromosomal aneuploidy detection chromosome instability copy number variations precancerous lesions of gastric cancer gene |
| title | Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer |
| title_full | Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer |
| title_fullStr | Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer |
| title_full_unstemmed | Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer |
| title_short | Detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer |
| title_sort | detection of chromosomal instability using ultrasensitive chromosomal aneuploidy detection in the diagnosis of precancerous lesions of gastric cancer |
| topic | ultrasensitive chromosomal aneuploidy detection chromosome instability copy number variations precancerous lesions of gastric cancer gene |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1359231/full |
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