γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation
γδ-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2+ and CD2- subsets of γδ-TCR lymphocytes were analy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2006-05-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200605-0010_gamma-delta-tcr-cd2-lymphocytes-are-recruited-into-bovine-mammary-gland-after-stimulation.php |
| _version_ | 1797897766470418432 |
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| author | M. Faldyna L. Leva Z. Sladek D. Rysanek M. Toman |
| author_facet | M. Faldyna L. Leva Z. Sladek D. Rysanek M. Toman |
| author_sort | M. Faldyna |
| collection | DOAJ |
| description | γδ-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2+ and CD2- subsets of γδ-TCR lymphocytes were analyzed. CD2+ γδ-TCR lymphocytes markedly prevailed over CD2- cells in intact mammary gland: 88.9 ± 4.9% of γδ-TCR lymphocytes were CD2+. After stimulation, neutrophils and γδ-TCR lymphocytes were recruited into the mammary gland. Among γδ-TCR lymphocytes, CD2- cells were mainly responsible for their expansion. After stimulation, 60.8 ± 13.4% of γδ-TCR lymphocytes were CD2+ (P < 0.01 when compared with mammary gland lavages before stimulation). It follows from the present study that the cells seem to be involved in the first phase of a response to an infection affecting mammary gland. |
| first_indexed | 2024-04-10T08:02:27Z |
| format | Article |
| id | doaj.art-f3f5f75327724272a86533368724d8f8 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:27Z |
| publishDate | 2006-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-f3f5f75327724272a86533368724d8f82023-02-23T03:49:21ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922006-05-0151525826410.17221/5545-VETMEDvet-200605-0010γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulationM. Faldyna0L. Leva1Z. Sladek2D. Rysanek3M. Toman4Veterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech Republicγδ-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2+ and CD2- subsets of γδ-TCR lymphocytes were analyzed. CD2+ γδ-TCR lymphocytes markedly prevailed over CD2- cells in intact mammary gland: 88.9 ± 4.9% of γδ-TCR lymphocytes were CD2+. After stimulation, neutrophils and γδ-TCR lymphocytes were recruited into the mammary gland. Among γδ-TCR lymphocytes, CD2- cells were mainly responsible for their expansion. After stimulation, 60.8 ± 13.4% of γδ-TCR lymphocytes were CD2+ (P < 0.01 when compared with mammary gland lavages before stimulation). It follows from the present study that the cells seem to be involved in the first phase of a response to an infection affecting mammary gland.https://vetmed.agriculturejournals.cz/artkey/vet-200605-0010_gamma-delta-tcr-cd2-lymphocytes-are-recruited-into-bovine-mammary-gland-after-stimulation.phpcattleuddermastitis |
| spellingShingle | M. Faldyna L. Leva Z. Sladek D. Rysanek M. Toman γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation Veterinární Medicína cattle udder mastitis |
| title | γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation |
| title_full | γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation |
| title_fullStr | γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation |
| title_full_unstemmed | γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation |
| title_short | γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation |
| title_sort | γδ tcr cd2 lymphocytes are recruited into bovine mammary gland after stimulation |
| topic | cattle udder mastitis |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200605-0010_gamma-delta-tcr-cd2-lymphocytes-are-recruited-into-bovine-mammary-gland-after-stimulation.php |
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