Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19)
Background: Inflammatory cell death, PANoptosis, has been suggested to orchestrate the lymphocyte decrement among coronavirus disease-2019 (COVID-19) patients. The main aim of this study was to examine the differences in the expression of key genes related to inflammatory cell death and their correl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=102;epage=102;aulast=Heidarvand |
| _version_ | 1797649606124765184 |
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| author | Mohammad Heidarvand Reza Hosseini Mohammad Kazemi Alireza Andalib Ramin Sami Nahid Eskandari Behrooz Ghezelbash |
| author_facet | Mohammad Heidarvand Reza Hosseini Mohammad Kazemi Alireza Andalib Ramin Sami Nahid Eskandari Behrooz Ghezelbash |
| author_sort | Mohammad Heidarvand |
| collection | DOAJ |
| description | Background: Inflammatory cell death, PANoptosis, has been suggested to orchestrate the lymphocyte decrement among coronavirus disease-2019 (COVID-19) patients. The main aim of this study was to examine the differences in the expression of key genes related to inflammatory cell death and their correlation with lymphopenia in the mild and severe types of COVID-19 patients.
Materials and Methods: Eighty-eight patients (36 to 60 years old) with mild (n = 44) and severe (n = 44) types of COVID-19 were enrolled. The expression of key genes related to apoptosis (FAS-associated death domain protein, FADD), pyroptosis (ASC (apoptosis-associated speck-like protein containing caspase activation and recruitment domains (CARD)), the adapter protein ASC binds directly to caspase-1 and is critical for caspase-1 activation in response to a broad range of stimuli), and necroptosis (mixed lineage kinase domain-like, MLKL) genes were examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay, and compared between the groups. The serum levels of interleukin (IL)-6 were measured by enzyme-linked immunosorbent assay (ELISA) assay.
Results: A major increase in the expression of FADD, ASC, and MLKL-related genes in the severe type of patients was compared to the mild type of patients. The serum levels of IL-6 similarly indicated a significant increase in the severe type of the patients. A significant negative correlation was detected between the three genes' expression and the levels of IL-6 with the lymphocyte counts in both types of COVID-19 patients.
Conclusion: Overall, the main regulated cell-death pathways are likely to be involved in lymphopenia in COVID-19 patients, and the expression levels of these genes could potentially predict the patients' outcome. |
| first_indexed | 2024-03-11T15:48:33Z |
| format | Article |
| id | doaj.art-f3f6a035d95f42a3ad36d69b45c1f873 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 |
| language | English |
| last_indexed | 2024-03-11T15:48:33Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-f3f6a035d95f42a3ad36d69b45c1f8732023-10-26T05:43:16ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752023-01-0112110210210.4103/abr.abr_232_22Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19)Mohammad HeidarvandReza HosseiniMohammad KazemiAlireza AndalibRamin SamiNahid EskandariBehrooz GhezelbashBackground: Inflammatory cell death, PANoptosis, has been suggested to orchestrate the lymphocyte decrement among coronavirus disease-2019 (COVID-19) patients. The main aim of this study was to examine the differences in the expression of key genes related to inflammatory cell death and their correlation with lymphopenia in the mild and severe types of COVID-19 patients. Materials and Methods: Eighty-eight patients (36 to 60 years old) with mild (n = 44) and severe (n = 44) types of COVID-19 were enrolled. The expression of key genes related to apoptosis (FAS-associated death domain protein, FADD), pyroptosis (ASC (apoptosis-associated speck-like protein containing caspase activation and recruitment domains (CARD)), the adapter protein ASC binds directly to caspase-1 and is critical for caspase-1 activation in response to a broad range of stimuli), and necroptosis (mixed lineage kinase domain-like, MLKL) genes were examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay, and compared between the groups. The serum levels of interleukin (IL)-6 were measured by enzyme-linked immunosorbent assay (ELISA) assay. Results: A major increase in the expression of FADD, ASC, and MLKL-related genes in the severe type of patients was compared to the mild type of patients. The serum levels of IL-6 similarly indicated a significant increase in the severe type of the patients. A significant negative correlation was detected between the three genes' expression and the levels of IL-6 with the lymphocyte counts in both types of COVID-19 patients. Conclusion: Overall, the main regulated cell-death pathways are likely to be involved in lymphopenia in COVID-19 patients, and the expression levels of these genes could potentially predict the patients' outcome.http://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=102;epage=102;aulast=Heidarvandapoptosiscoronavirus disease-19interleukin-6lymphopenianecroptosispyroptosis |
| spellingShingle | Mohammad Heidarvand Reza Hosseini Mohammad Kazemi Alireza Andalib Ramin Sami Nahid Eskandari Behrooz Ghezelbash Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19) Advanced Biomedical Research apoptosis coronavirus disease-19 interleukin-6 lymphopenia necroptosis pyroptosis |
| title | Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19) |
| title_full | Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19) |
| title_fullStr | Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19) |
| title_full_unstemmed | Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19) |
| title_short | Differentially expressed inflammatory cell death-related genes and the serum levels of IL-6 are determinants for severity of coronaviruses diseases-2019 (COVID-19) |
| title_sort | differentially expressed inflammatory cell death related genes and the serum levels of il 6 are determinants for severity of coronaviruses diseases 2019 covid 19 |
| topic | apoptosis coronavirus disease-19 interleukin-6 lymphopenia necroptosis pyroptosis |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=102;epage=102;aulast=Heidarvand |
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