Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients
The relationship between the nasopharyngeal virus load, IgA and IgG antibodies to both the S1-RBD-protein and the N-protein, as well as the neutralizing activity (NAbs) against SARS-CoV-2 in the blood of moderately afflicted COVID-19 patients, needs further longitudinal investigation. Several new se...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/12/1357 |
| _version_ | 1797546640663379968 |
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| author | Bertram Flehmig Michael Schindler Natalia Ruetalo Ramona Businger Manfred Bayer Angelika Haage Thomas Kirchner Karin Klingel Andrea Normann Lutz Pridzun Despina Tougianidou Michael B. Ranke |
| author_facet | Bertram Flehmig Michael Schindler Natalia Ruetalo Ramona Businger Manfred Bayer Angelika Haage Thomas Kirchner Karin Klingel Andrea Normann Lutz Pridzun Despina Tougianidou Michael B. Ranke |
| author_sort | Bertram Flehmig |
| collection | DOAJ |
| description | The relationship between the nasopharyngeal virus load, IgA and IgG antibodies to both the S1-RBD-protein and the N-protein, as well as the neutralizing activity (NAbs) against SARS-CoV-2 in the blood of moderately afflicted COVID-19 patients, needs further longitudinal investigation. Several new serological methods to examine these parameters were developed, validated and applied in three patients of a family which underwent an ambulatory course of COVID-19 for six months. The virus load had almost completely disappeared after about four weeks. Serum IgA levels to the S1-RBD-protein and, to a lesser extent, to the N-protein, peaked about three weeks after clinical disease onset but declined soon thereafter. IgG levels rose continuously, reaching a plateau at approximately six weeks, and stayed elevated over the observation period. Virus-neutralizing activity reached a peak about 4 weeks after disease onset but dropped slowly. The longitudinal associations of virus neutralization and the serological immune response suggest immunity in patients even after a mild clinical course of COVID-19. |
| first_indexed | 2024-03-10T14:32:34Z |
| format | Article |
| id | doaj.art-f3f76e2ee5e642d5af3dde3fd544e4a3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T14:32:34Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f3f76e2ee5e642d5af3dde3fd544e4a32023-11-20T22:32:00ZengMDPI AGViruses1999-49152020-11-011212135710.3390/v12121357Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 PatientsBertram Flehmig0Michael Schindler1Natalia Ruetalo2Ramona Businger3Manfred Bayer4Angelika Haage5Thomas Kirchner6Karin Klingel7Andrea Normann8Lutz Pridzun9Despina Tougianidou10Michael B. Ranke11Paediatric Endocrinolgy, University Children’s Hospital, Hoppe-Seyler-Str. 1, 72076 Tuebingen, GermanyInstitute for Medical Virology and Epidemiology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, GermanyInstitute for Medical Virology and Epidemiology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, GermanyInstitute for Medical Virology and Epidemiology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, Germany4base Lab AG Advanced Molecular Analysis, Aspenhaustr. 25, 72770 Reutlingen, GermanyMediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Aspenhaustr. 25, 72770 Reutlingen, GermanyPediatric Practice, Kapuzinerberg 17, 71263 Weil der Stadt, GermanyInstitute for Pathology and Neuropathology, University Hospital Tuebingen, Liebermeisterstr. 8, 72076 Tuebingen, GermanyMediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Aspenhaustr. 25, 72770 Reutlingen, GermanyMediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH, Aspenhaustr. 25, 72770 Reutlingen, Germany4base Lab AG Advanced Molecular Analysis, Aspenhaustr. 25, 72770 Reutlingen, GermanyPaediatric Endocrinolgy, University Children’s Hospital, Hoppe-Seyler-Str. 1, 72076 Tuebingen, GermanyThe relationship between the nasopharyngeal virus load, IgA and IgG antibodies to both the S1-RBD-protein and the N-protein, as well as the neutralizing activity (NAbs) against SARS-CoV-2 in the blood of moderately afflicted COVID-19 patients, needs further longitudinal investigation. Several new serological methods to examine these parameters were developed, validated and applied in three patients of a family which underwent an ambulatory course of COVID-19 for six months. The virus load had almost completely disappeared after about four weeks. Serum IgA levels to the S1-RBD-protein and, to a lesser extent, to the N-protein, peaked about three weeks after clinical disease onset but declined soon thereafter. IgG levels rose continuously, reaching a plateau at approximately six weeks, and stayed elevated over the observation period. Virus-neutralizing activity reached a peak about 4 weeks after disease onset but dropped slowly. The longitudinal associations of virus neutralization and the serological immune response suggest immunity in patients even after a mild clinical course of COVID-19.https://www.mdpi.com/1999-4915/12/12/1357COVID-19SARS-CoV-2 RT-qPCRanti-SARS-CoV-2 antibodiesSARS-CoV-2 S1-RDB proteinSARS-CoV-2 N-proteinSARS-CoV-2 neutralizing activity |
| spellingShingle | Bertram Flehmig Michael Schindler Natalia Ruetalo Ramona Businger Manfred Bayer Angelika Haage Thomas Kirchner Karin Klingel Andrea Normann Lutz Pridzun Despina Tougianidou Michael B. Ranke Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients Viruses COVID-19 SARS-CoV-2 RT-qPCR anti-SARS-CoV-2 antibodies SARS-CoV-2 S1-RDB protein SARS-CoV-2 N-protein SARS-CoV-2 neutralizing activity |
| title | Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients |
| title_full | Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients |
| title_fullStr | Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients |
| title_full_unstemmed | Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients |
| title_short | Persisting Neutralizing Activity to SARS-CoV-2 over Months in Sera of COVID-19 Patients |
| title_sort | persisting neutralizing activity to sars cov 2 over months in sera of covid 19 patients |
| topic | COVID-19 SARS-CoV-2 RT-qPCR anti-SARS-CoV-2 antibodies SARS-CoV-2 S1-RDB protein SARS-CoV-2 N-protein SARS-CoV-2 neutralizing activity |
| url | https://www.mdpi.com/1999-4915/12/12/1357 |
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