Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats
Mercury is a highly toxic metal. It induces its toxicity via production of reactive oxygen species. Brain tissues are more susceptible to oxidative damage. Melatonin and its metabolites are free radical scavengers. The aim of this work is to elucidate the neuroprotective effect of melatonin on mercu...
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Elsevier
2021-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021015887 |
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| author | Eman S. Said Radwa M. Ahmed Rehab A. Mohammed Enas M. Morsi Mohamed H. Elmahdi Hassan S. Elsayed Rania H. Mahmoud Eman H. Nadwa |
| author_facet | Eman S. Said Radwa M. Ahmed Rehab A. Mohammed Enas M. Morsi Mohamed H. Elmahdi Hassan S. Elsayed Rania H. Mahmoud Eman H. Nadwa |
| author_sort | Eman S. Said |
| collection | DOAJ |
| description | Mercury is a highly toxic metal. It induces its toxicity via production of reactive oxygen species. Brain tissues are more susceptible to oxidative damage. Melatonin and its metabolites are free radical scavengers. The aim of this work is to elucidate the neuroprotective effect of melatonin on mercuric chloride-induced neurotoxicity in rats. Fifty male albino rats were used and divided into five groups. Group I acts as normal control. Group II (LD HgCl2) received mercuric chloride at a dose of 2 mg/kg. Group III (HD HgCl2) received HgCl2 at a dose of 4 mg/kg. Rats in group IV (LD HgCl2 +MLT) received HgCl2 2 mg/kg + Melatonin 5 mg/kg. Rats in group V (HD HgCl2+MLT) received HgCl2 4 mg/kg + Melatonin5 mg/kg. This study revealed that mercuric chloride decreased the activity of superoxide dismutase, catalase and glutathione peroxidase enzymes and increased malondialdehyde levels. Toxicity of mercuric chloride lead to upregulation of the gene expression level vascular endothelial growth factor. HgCl2 induced fragmentation of rough endoplasmic reticulum, ballooning of Golgi apparatus, nuclear and cytoplasmic degeneration of pyramidal neurones of rat cerebral cortex. This neuronal damage caused by HgCl2 was significantly improved by melatonin. |
| first_indexed | 2024-12-22T10:53:37Z |
| format | Article |
| id | doaj.art-f3fa3cbbb8af40979b3424067605c6c4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T10:53:37Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f3fa3cbbb8af40979b3424067605c6c42022-12-21T18:28:41ZengElsevierHeliyon2405-84402021-07-0177e07485Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in ratsEman S. Said0Radwa M. Ahmed1Rehab A. Mohammed2Enas M. Morsi3Mohamed H. Elmahdi4Hassan S. Elsayed5Rania H. Mahmoud6Eman H. Nadwa7Department of Clinical Pharmacology, Faculty of Medicine, Fayoum University, Fayoum, Egypt; Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Saudi Arabia; Corresponding author.Department of Anatomy and Embryology, Faculty of Medicine, Fayoum University, EgyptDepartment of Medical Physiology, Faculty of Medicine, Fayoum University, EgyptDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Fayoum University, EgyptDepartment of Pathology, Faculty of Medicine, Fayoum University, EgyptDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, EgyptDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, EgyptDepartment of Pharmacology and Therapeutics, College of Medicine, Jouf University, Sakaka, Saudi Arabia; Department of Medical Pharmacology, Faculty of Medicine, Cairo University, Giza, EgyptMercury is a highly toxic metal. It induces its toxicity via production of reactive oxygen species. Brain tissues are more susceptible to oxidative damage. Melatonin and its metabolites are free radical scavengers. The aim of this work is to elucidate the neuroprotective effect of melatonin on mercuric chloride-induced neurotoxicity in rats. Fifty male albino rats were used and divided into five groups. Group I acts as normal control. Group II (LD HgCl2) received mercuric chloride at a dose of 2 mg/kg. Group III (HD HgCl2) received HgCl2 at a dose of 4 mg/kg. Rats in group IV (LD HgCl2 +MLT) received HgCl2 2 mg/kg + Melatonin 5 mg/kg. Rats in group V (HD HgCl2+MLT) received HgCl2 4 mg/kg + Melatonin5 mg/kg. This study revealed that mercuric chloride decreased the activity of superoxide dismutase, catalase and glutathione peroxidase enzymes and increased malondialdehyde levels. Toxicity of mercuric chloride lead to upregulation of the gene expression level vascular endothelial growth factor. HgCl2 induced fragmentation of rough endoplasmic reticulum, ballooning of Golgi apparatus, nuclear and cytoplasmic degeneration of pyramidal neurones of rat cerebral cortex. This neuronal damage caused by HgCl2 was significantly improved by melatonin.http://www.sciencedirect.com/science/article/pii/S2405844021015887Mercuric chlorideMelatoninCerebral cortexOxidative stressNeurotoxicity |
| spellingShingle | Eman S. Said Radwa M. Ahmed Rehab A. Mohammed Enas M. Morsi Mohamed H. Elmahdi Hassan S. Elsayed Rania H. Mahmoud Eman H. Nadwa Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats Heliyon Mercuric chloride Melatonin Cerebral cortex Oxidative stress Neurotoxicity |
| title | Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats |
| title_full | Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats |
| title_fullStr | Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats |
| title_full_unstemmed | Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats |
| title_short | Ameliorating effect of melatonin on mercuric chloride-induced neurotoxicity in rats |
| title_sort | ameliorating effect of melatonin on mercuric chloride induced neurotoxicity in rats |
| topic | Mercuric chloride Melatonin Cerebral cortex Oxidative stress Neurotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021015887 |
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