Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait
Maize is the main staple food of the world but it is nutritional deficient due to the scarcity of two essential amino acids viz., lysine and tryptophan. Under this study, opaque2 gene from QPM donor (CML-169) was transferred into normal maize (BAJIM-08-26) through marker aided backcross breeding m...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2022-06-01
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| Series: | Electronic Journal of Plant Breeding |
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| Online Access: | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4062 |
| _version_ | 1797852484147871744 |
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| author | Swaran Lata1, Reena Kumari*2, Kalpna Thakur1, Maneet Rana3, Naresh Thakur2 and Waseem Hussain4 |
| author_facet | Swaran Lata1, Reena Kumari*2, Kalpna Thakur1, Maneet Rana3, Naresh Thakur2 and Waseem Hussain4 |
| author_sort | Swaran Lata1, Reena Kumari*2, Kalpna Thakur1, Maneet Rana3, Naresh Thakur2 and Waseem Hussain4 |
| collection | DOAJ |
| description | Maize is the main staple food of the world but it is nutritional deficient due to the scarcity of two essential amino acids
viz., lysine and tryptophan. Under this study, opaque2 gene from QPM donor (CML-169) was transferred into normal
maize (BAJIM-08-26) through marker aided backcross breeding method. The opaque2 specific SSR marker (phi057)
was used for foreground selection in BC1F1 and BC2F1 generations. The heterozygous offspring were self-pollinated to
produce the BC2F2 generation. Further plants on the basis of 25% opaqueness were selected and selfed to develop
BC2F3 and BC2F4 progenies. The background selection using a series of SSR markers showed 97% recovery in the
recurrent parent genome of backcrossed generation. Tryptophan content and total protein in the endosperm of BC2F4
progenies were found to be ranged from 0.75 to 0.93% and 7.0 to 9.6 %, respectively. As an outcome, three promising
MAS derived QPM lines viz., B76-22-2, B70-5-8 and B42-3-1 were developed that showed a high percentage of
tryptophan, grain yield and recovery of recurrent parent genome. The worldwide problem of protein malnutrition
could be improved by adopting a molecular breeding approach for the development of nutritionally rich maize over the
normal maize. |
| first_indexed | 2024-04-09T19:35:06Z |
| format | Article |
| id | doaj.art-f4000457784f46cd9bb763b954213b19 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-04-09T19:35:06Z |
| publishDate | 2022-06-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-f4000457784f46cd9bb763b954213b192023-04-04T16:12:51ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2022-06-0113229530410.37992/2022.1302.082Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 traitSwaran Lata1, Reena Kumari*2, Kalpna Thakur1, Maneet Rana3, Naresh Thakur2 and Waseem Hussain401Department of Crop Improvement, College of Agriculture,CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Himachal Pradesh, India, 176062, 2Department of Biotechnology, College of Horticulture and Forestry, Neri, Hamirpur (HP), 177001, 3Department of Crop Improvement, ICAR-Indian Grassland and Fodder Research Institute, Jhansi (UP), 284 003, 4Department of Agronomy and Horticulture, University of Nebraska, Lincoln- 68583-0915, USA *E-Mail: reenarattan@gmail.comMaize is the main staple food of the world but it is nutritional deficient due to the scarcity of two essential amino acids viz., lysine and tryptophan. Under this study, opaque2 gene from QPM donor (CML-169) was transferred into normal maize (BAJIM-08-26) through marker aided backcross breeding method. The opaque2 specific SSR marker (phi057) was used for foreground selection in BC1F1 and BC2F1 generations. The heterozygous offspring were self-pollinated to produce the BC2F2 generation. Further plants on the basis of 25% opaqueness were selected and selfed to develop BC2F3 and BC2F4 progenies. The background selection using a series of SSR markers showed 97% recovery in the recurrent parent genome of backcrossed generation. Tryptophan content and total protein in the endosperm of BC2F4 progenies were found to be ranged from 0.75 to 0.93% and 7.0 to 9.6 %, respectively. As an outcome, three promising MAS derived QPM lines viz., B76-22-2, B70-5-8 and B42-3-1 were developed that showed a high percentage of tryptophan, grain yield and recovery of recurrent parent genome. The worldwide problem of protein malnutrition could be improved by adopting a molecular breeding approach for the development of nutritionally rich maize over the normal maize.https://www.ejplantbreeding.org/index.php/EJPB/article/view/4062marker assisted backcrossingopaque2tryptophan contentssr markersqpm lines and normal maize |
| spellingShingle | Swaran Lata1, Reena Kumari*2, Kalpna Thakur1, Maneet Rana3, Naresh Thakur2 and Waseem Hussain4 Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait Electronic Journal of Plant Breeding marker assisted backcrossing opaque2 tryptophan content ssr markers qpm lines and normal maize |
| title | Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait |
| title_full | Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait |
| title_fullStr | Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait |
| title_full_unstemmed | Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait |
| title_short | Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait |
| title_sort | development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of opaque2 trait |
| topic | marker assisted backcrossing opaque2 tryptophan content ssr markers qpm lines and normal maize |
| url | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4062 |
| work_keys_str_mv | AT swaranlata1reenakumari2kalpnathakur1maneetrana3nareshthakur2andwaseemhussain4 developmentoftryptophanrichmaizelinesthroughsimplesequencerepeatmarkeraidedintrogressionofopaque2trait |