<i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
<i>Lactobacillus</i> strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals’ gut health and production performance. In this study, we isolated 57 <i>Lactobacillus</i> strains from the gut microbiota of 17 different chi...
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/726 |
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| author | Chang Xu Fuxiao Wei Xinyue Yang Yuqing Feng Dan Liu Yongfei Hu |
| author_facet | Chang Xu Fuxiao Wei Xinyue Yang Yuqing Feng Dan Liu Yongfei Hu |
| author_sort | Chang Xu |
| collection | DOAJ |
| description | <i>Lactobacillus</i> strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals’ gut health and production performance. In this study, we isolated 57 <i>Lactobacillus</i> strains from the gut microbiota of 17 different chicken breeds in China. We characterized the probiotic features of these isolates, and evaluated the effects of a selected strain, <i>Lactobacillus salivarius</i> CML352, on the production performance and gut health of the late-phase laying hens. The results showed that the isolates varied much in probiotic properties, among which <i>L. salivarius</i> CML352 displayed high acid and bile salt tolerance, high hydrophobicity, auto-aggregation, and antibacterial activities. Whole genome sequencing analysis showed that CML352 was closely related to a strain isolated from human fecal samples, but had different functional potentials. Dietary supplementary of <i>L. salivarius</i> CML352 significantly reduced the Firmicutes to Bacteroidetes ratio, increased the expression of <i>Muc-2</i>, and decreased the expression of <i>MyD88</i>, <i>IFN-γ</i>, and <i>TLR-4</i>. Furthermore, strain CML352 reduced the birds’ abdominal fat deposition, and improved egg quality. Taken together, this study indicated that the newly isolated <i>L. salivarius</i> strain might be a worthy probiotic with positive impacts on the intestinal health and production performance of late-phase laying hens. |
| first_indexed | 2024-03-09T13:16:59Z |
| format | Article |
| id | doaj.art-f403b9bd079d4645827ad24d62be7230 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:59Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f403b9bd079d4645827ad24d62be72302023-11-30T21:34:59ZengMDPI AGMicroorganisms2076-26072022-03-0110472610.3390/microorganisms10040726<i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying HensChang Xu0Fuxiao Wei1Xinyue Yang2Yuqing Feng3Dan Liu4Yongfei Hu5State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China<i>Lactobacillus</i> strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals’ gut health and production performance. In this study, we isolated 57 <i>Lactobacillus</i> strains from the gut microbiota of 17 different chicken breeds in China. We characterized the probiotic features of these isolates, and evaluated the effects of a selected strain, <i>Lactobacillus salivarius</i> CML352, on the production performance and gut health of the late-phase laying hens. The results showed that the isolates varied much in probiotic properties, among which <i>L. salivarius</i> CML352 displayed high acid and bile salt tolerance, high hydrophobicity, auto-aggregation, and antibacterial activities. Whole genome sequencing analysis showed that CML352 was closely related to a strain isolated from human fecal samples, but had different functional potentials. Dietary supplementary of <i>L. salivarius</i> CML352 significantly reduced the Firmicutes to Bacteroidetes ratio, increased the expression of <i>Muc-2</i>, and decreased the expression of <i>MyD88</i>, <i>IFN-γ</i>, and <i>TLR-4</i>. Furthermore, strain CML352 reduced the birds’ abdominal fat deposition, and improved egg quality. Taken together, this study indicated that the newly isolated <i>L. salivarius</i> strain might be a worthy probiotic with positive impacts on the intestinal health and production performance of late-phase laying hens.https://www.mdpi.com/2076-2607/10/4/726<i>Lactobacillus salivarius</i>laying hensgut microbiotaegg qualityintestinal health |
| spellingShingle | Chang Xu Fuxiao Wei Xinyue Yang Yuqing Feng Dan Liu Yongfei Hu <i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens Microorganisms <i>Lactobacillus salivarius</i> laying hens gut microbiota egg quality intestinal health |
| title | <i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens |
| title_full | <i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens |
| title_fullStr | <i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens |
| title_full_unstemmed | <i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens |
| title_short | <i>Lactobacillus salivarius</i> CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens |
| title_sort | i lactobacillus salivarius i cml352 isolated from chinese local breed chicken modulates the gut microbiota and improves intestinal health and egg quality in late phase laying hens |
| topic | <i>Lactobacillus salivarius</i> laying hens gut microbiota egg quality intestinal health |
| url | https://www.mdpi.com/2076-2607/10/4/726 |
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