The effect of ginger extract on cisplatin-induced acute anorexia in rats
Cisplatin is a platinum-based chemotherapeutic agent widely used to treat various cancers. However, several side effects have been reported in treated patients. Among these, acute anorexia is one of the most severe secondary effects. In this study, a single oral administration of 100 or 500 mg/kg gi...
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1267254/full |
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| author | Hyeonah Kim Keun-Tae Park Heejoon Jo Yuchan Shin Geehoon Chung Seong-Gyu Ko Young-Ho Jin Woojin Kim Woojin Kim |
| author_facet | Hyeonah Kim Keun-Tae Park Heejoon Jo Yuchan Shin Geehoon Chung Seong-Gyu Ko Young-Ho Jin Woojin Kim Woojin Kim |
| author_sort | Hyeonah Kim |
| collection | DOAJ |
| description | Cisplatin is a platinum-based chemotherapeutic agent widely used to treat various cancers. However, several side effects have been reported in treated patients. Among these, acute anorexia is one of the most severe secondary effects. In this study, a single oral administration of 100 or 500 mg/kg ginger extract (GE) significantly alleviated the cisplatin-induced decrease in food intake in rats. However, these body weight and water intake decreases were reversed in the 100 mg/kg group rats. To elucidate the underlying mechanism of action, serotonin (5-HT) and 5-HT2C, 3A, and 4 receptors in the nodose ganglion of the vagus nerve were investigated. The results showed that cisplatin-induced increases in serotonin levels in both the blood and nodose ganglion tissues were significantly decreased by100 and 500 mg/kg of GE administration. On 5-HT receptors, 5-HT3A and 4, but not 2C receptors, were affected by cisplatin, and GE 100 and 500 mg/kg succeeded in downregulating the evoked upregulated gene of these receptors. Protein expression of 5-HT3A and 4 receptors were also reduced in the 100 mg/kg group. Furthermore, the injection of 5-HT3A, and 4 receptors antagonists (palonostron, 0.1 mg/kg, i.p.; piboserod, 1 mg/kg, i.p., respectively) in cisplatin treated rats prevented the decrease in food intake. Using high-performance liquid chromatography (HPLC) analysis, [6]-gingerol and [6]-shogaol were identified and quantified as the major components of GE, comprising 4.12% and 2.15% of the GE, respectively. Although [6]-gingerol or [6]-shogaol alone failed to alleviate the evoked anorexia, when treated together, the effect was significant on the cisplatin-induced decrease in food intake. These results show that GE can be considered a treatment option to alleviate cisplatin-induced anorexia. |
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| format | Article |
| id | doaj.art-f406ad111e48453589274c716069ec2e |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-11T11:12:04Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-f406ad111e48453589274c716069ec2e2023-11-11T21:58:48ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-11-011410.3389/fphar.2023.12672541267254The effect of ginger extract on cisplatin-induced acute anorexia in ratsHyeonah Kim0Keun-Tae Park1Heejoon Jo2Yuchan Shin3Geehoon Chung4Seong-Gyu Ko5Young-Ho Jin6Woojin Kim7Woojin Kim8Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaKorean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Physiology, College of Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaKorean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaCisplatin is a platinum-based chemotherapeutic agent widely used to treat various cancers. However, several side effects have been reported in treated patients. Among these, acute anorexia is one of the most severe secondary effects. In this study, a single oral administration of 100 or 500 mg/kg ginger extract (GE) significantly alleviated the cisplatin-induced decrease in food intake in rats. However, these body weight and water intake decreases were reversed in the 100 mg/kg group rats. To elucidate the underlying mechanism of action, serotonin (5-HT) and 5-HT2C, 3A, and 4 receptors in the nodose ganglion of the vagus nerve were investigated. The results showed that cisplatin-induced increases in serotonin levels in both the blood and nodose ganglion tissues were significantly decreased by100 and 500 mg/kg of GE administration. On 5-HT receptors, 5-HT3A and 4, but not 2C receptors, were affected by cisplatin, and GE 100 and 500 mg/kg succeeded in downregulating the evoked upregulated gene of these receptors. Protein expression of 5-HT3A and 4 receptors were also reduced in the 100 mg/kg group. Furthermore, the injection of 5-HT3A, and 4 receptors antagonists (palonostron, 0.1 mg/kg, i.p.; piboserod, 1 mg/kg, i.p., respectively) in cisplatin treated rats prevented the decrease in food intake. Using high-performance liquid chromatography (HPLC) analysis, [6]-gingerol and [6]-shogaol were identified and quantified as the major components of GE, comprising 4.12% and 2.15% of the GE, respectively. Although [6]-gingerol or [6]-shogaol alone failed to alleviate the evoked anorexia, when treated together, the effect was significant on the cisplatin-induced decrease in food intake. These results show that GE can be considered a treatment option to alleviate cisplatin-induced anorexia.https://www.frontiersin.org/articles/10.3389/fphar.2023.1267254/fullanorexiachemotherapy-induced anorexiacisplatingingernodose ganglionserotonin |
| spellingShingle | Hyeonah Kim Keun-Tae Park Heejoon Jo Yuchan Shin Geehoon Chung Seong-Gyu Ko Young-Ho Jin Woojin Kim Woojin Kim The effect of ginger extract on cisplatin-induced acute anorexia in rats Frontiers in Pharmacology anorexia chemotherapy-induced anorexia cisplatin ginger nodose ganglion serotonin |
| title | The effect of ginger extract on cisplatin-induced acute anorexia in rats |
| title_full | The effect of ginger extract on cisplatin-induced acute anorexia in rats |
| title_fullStr | The effect of ginger extract on cisplatin-induced acute anorexia in rats |
| title_full_unstemmed | The effect of ginger extract on cisplatin-induced acute anorexia in rats |
| title_short | The effect of ginger extract on cisplatin-induced acute anorexia in rats |
| title_sort | effect of ginger extract on cisplatin induced acute anorexia in rats |
| topic | anorexia chemotherapy-induced anorexia cisplatin ginger nodose ganglion serotonin |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1267254/full |
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