Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro
Background: Muscular atrophy (MA) is a disease of various origins, i.e., genetic or the most common, caused by mechanical injury. So far, there is no universal therapeutic model because this disease is often progressive with numerous manifested symptoms. Moreover, there is no safe and low-risk thera...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016422001554 |
| _version_ | 1828156845210992640 |
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| author | Maksymilian Wastag Katarzyna Bieżuńska-Kusiak Anna Szewczyk Wojciech Szlasa Bożena Grimling Julita Kulbacka |
| author_facet | Maksymilian Wastag Katarzyna Bieżuńska-Kusiak Anna Szewczyk Wojciech Szlasa Bożena Grimling Julita Kulbacka |
| author_sort | Maksymilian Wastag |
| collection | DOAJ |
| description | Background: Muscular atrophy (MA) is a disease of various origins, i.e., genetic or the most common, caused by mechanical injury. So far, there is no universal therapeutic model because this disease is often progressive with numerous manifested symptoms. Moreover, there is no safe and low-risk therapy dedicated to muscle atrophy. For this reason, our research focuses on finding an alternative method using natural compounds to treat MA. This study proposes implementing natural substances such as celastrol and Rhynchophylline on the cellular level, using a simulated and controlled atrophy process. Methods: Celastrol and Rhynchophylline were used as natural compounds against simulated atrophy in C2C12 cells. Skeletal muscle C2C12 cells were stimulated for the differentiation process. Atrophic conditions were obtained by the exposure to the low concertation of doxorubicin and validated by FoxO3 and MAFbx. The protective and regenerative effect of drugs on cell proliferation was determined by the MTT assay and MT-CO1, VDAC1, and prohibitin expression. Results: The obtained results revealed that both natural substances reduced atrophic symptoms. Rhynchophylline and celastrol attenuated atrophic cells in the viability studies, morphology analysis by diameter measurements, modulated prohibitin VDAC, and MT-CO1 expression. Conclusions: The obtained results revealed that celastrol and Rhynchophylline could be effectively used as a supportive treatment in atrophy-related disorders. Thus, natural drugs seem promising for muscle regeneration. |
| first_indexed | 2024-04-11T23:18:14Z |
| format | Article |
| id | doaj.art-f40b412fffef42fa90517f6add140247 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-11T23:18:14Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-f40b412fffef42fa90517f6add1402472022-12-22T03:57:32ZengElsevierSaudi Pharmaceutical Journal1319-01642022-10-01301013871395Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitroMaksymilian Wastag0Katarzyna Bieżuńska-Kusiak1Anna Szewczyk2Wojciech Szlasa3Bożena Grimling4Julita Kulbacka5Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556, Wroclaw, PolandDepartment of Molecular and Cellular Biology, Wroclaw Medical University, Borowska 211A 50-556, Wroclaw, PolandDepartment of Molecular and Cellular Biology, Wroclaw Medical University, Borowska 211A 50-556, Wroclaw, Poland; Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21, 50-335 Wroclaw, PolandFaculty of Medicine, Wroclaw Medical University, Pasteura 1, 50-367 Wroclaw, PolandDepartment of Drug Form Technology, Wroclaw Medical University, Borowska 211 A, 50-556 Wroclaw, PolandDepartment of Molecular and Cellular Biology, Wroclaw Medical University, Borowska 211A 50-556, Wroclaw, Poland; Corresponding author at: Borowska 211A, 50-556 Wroclaw, Poland.Background: Muscular atrophy (MA) is a disease of various origins, i.e., genetic or the most common, caused by mechanical injury. So far, there is no universal therapeutic model because this disease is often progressive with numerous manifested symptoms. Moreover, there is no safe and low-risk therapy dedicated to muscle atrophy. For this reason, our research focuses on finding an alternative method using natural compounds to treat MA. This study proposes implementing natural substances such as celastrol and Rhynchophylline on the cellular level, using a simulated and controlled atrophy process. Methods: Celastrol and Rhynchophylline were used as natural compounds against simulated atrophy in C2C12 cells. Skeletal muscle C2C12 cells were stimulated for the differentiation process. Atrophic conditions were obtained by the exposure to the low concertation of doxorubicin and validated by FoxO3 and MAFbx. The protective and regenerative effect of drugs on cell proliferation was determined by the MTT assay and MT-CO1, VDAC1, and prohibitin expression. Results: The obtained results revealed that both natural substances reduced atrophic symptoms. Rhynchophylline and celastrol attenuated atrophic cells in the viability studies, morphology analysis by diameter measurements, modulated prohibitin VDAC, and MT-CO1 expression. Conclusions: The obtained results revealed that celastrol and Rhynchophylline could be effectively used as a supportive treatment in atrophy-related disorders. Thus, natural drugs seem promising for muscle regeneration.http://www.sciencedirect.com/science/article/pii/S1319016422001554Muscle atrophySkeletal musclesCelastrolRhynchophylline |
| spellingShingle | Maksymilian Wastag Katarzyna Bieżuńska-Kusiak Anna Szewczyk Wojciech Szlasa Bożena Grimling Julita Kulbacka Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro Saudi Pharmaceutical Journal Muscle atrophy Skeletal muscles Celastrol Rhynchophylline |
| title | Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro |
| title_full | Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro |
| title_fullStr | Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro |
| title_full_unstemmed | Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro |
| title_short | Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro |
| title_sort | celastrol and rhynchophylline in the mitigation of simulated muscle atrophy under in vitro |
| topic | Muscle atrophy Skeletal muscles Celastrol Rhynchophylline |
| url | http://www.sciencedirect.com/science/article/pii/S1319016422001554 |
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