MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks
Under caged stress conditions, severe disruptions in duck intestinal barrier function, which adversely affect economic performance, have been observed. MiRNAs play a crucial role in cellular processes, but the mechanisms underlying their involvement in repairing oxidative stress-induced damage to du...
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/7/1037 |
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| author | Li Zhang Xiang Luo Rui Tang Yan Wu Zhenhua Liang Jingbo Liu Jinsong Pi Hao Zhang |
| author_facet | Li Zhang Xiang Luo Rui Tang Yan Wu Zhenhua Liang Jingbo Liu Jinsong Pi Hao Zhang |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | Under caged stress conditions, severe disruptions in duck intestinal barrier function, which adversely affect economic performance, have been observed. MiRNAs play a crucial role in cellular processes, but the mechanisms underlying their involvement in repairing oxidative stress-induced damage to duck intestinal barriers have not been elucidated. We performed miRNA-seq and protein tandem mass tagging (TMT) sequencing and identified differentially expressed miRNAs and proteins in oxidative stress-treated ducks. Dual-luciferase reporter vector experiments, RT-qPCR, and Western blotting revealed the regulatory role of apla-miR-106a-5p/MAP3K2 in intestinal barrier damage repair. The results showed that oxidative stress led to shortened villi and deepened crypts, impairing intestinal immune function. Significant downregulation of apla-miR-106a-5p was revealed by miRNA-seq, and the inhibition of its expression not only enhanced cell viability but also improved intestinal barrier function. TMT protein sequencing revealed MAP3K2 upregulation in caged-stressed duck intestines, and software analysis confirmed <i>MAP3K2</i> as the target gene of apla-miR-106a-5p. Dual-fluorescence reporter gene experiments demonstrated direct targeting of <i>MAP3K2</i> by apla-miR-106a-5p. RT-qPCR showed no effect on <i>MAP3K2</i> expression, while Western blot analysis indicated that MAP3K2 protein expression was suppressed. In summary, apla-miR-106a-5p targets <i>MAP3K2</i>, regulating gene expression at the transcriptional level and facilitating effective repair of intestinal barrier damage. This discovery provides new insights into the molecular mechanisms of physiological damage in ducks under caged stress, offering valuable guidance for related research. |
| first_indexed | 2024-04-24T10:51:34Z |
| format | Article |
| id | doaj.art-f40d4f13ee874fa48d650869ac73c054 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-24T10:51:34Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-f40d4f13ee874fa48d650869ac73c0542024-04-12T13:14:11ZengMDPI AGAnimals2076-26152024-03-01147103710.3390/ani14071037MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying DucksLi Zhang0Xiang Luo1Rui Tang2Yan Wu3Zhenhua Liang4Jingbo Liu5Jinsong Pi6Hao Zhang7Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaUnder caged stress conditions, severe disruptions in duck intestinal barrier function, which adversely affect economic performance, have been observed. MiRNAs play a crucial role in cellular processes, but the mechanisms underlying their involvement in repairing oxidative stress-induced damage to duck intestinal barriers have not been elucidated. We performed miRNA-seq and protein tandem mass tagging (TMT) sequencing and identified differentially expressed miRNAs and proteins in oxidative stress-treated ducks. Dual-luciferase reporter vector experiments, RT-qPCR, and Western blotting revealed the regulatory role of apla-miR-106a-5p/MAP3K2 in intestinal barrier damage repair. The results showed that oxidative stress led to shortened villi and deepened crypts, impairing intestinal immune function. Significant downregulation of apla-miR-106a-5p was revealed by miRNA-seq, and the inhibition of its expression not only enhanced cell viability but also improved intestinal barrier function. TMT protein sequencing revealed MAP3K2 upregulation in caged-stressed duck intestines, and software analysis confirmed <i>MAP3K2</i> as the target gene of apla-miR-106a-5p. Dual-fluorescence reporter gene experiments demonstrated direct targeting of <i>MAP3K2</i> by apla-miR-106a-5p. RT-qPCR showed no effect on <i>MAP3K2</i> expression, while Western blot analysis indicated that MAP3K2 protein expression was suppressed. In summary, apla-miR-106a-5p targets <i>MAP3K2</i>, regulating gene expression at the transcriptional level and facilitating effective repair of intestinal barrier damage. This discovery provides new insights into the molecular mechanisms of physiological damage in ducks under caged stress, offering valuable guidance for related research.https://www.mdpi.com/2076-2615/14/7/1037apla-miR-106a-5pintestinal barrier functionMAP3K2oxidative stressprelaying ducks |
| spellingShingle | Li Zhang Xiang Luo Rui Tang Yan Wu Zhenhua Liang Jingbo Liu Jinsong Pi Hao Zhang MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks Animals apla-miR-106a-5p intestinal barrier function MAP3K2 oxidative stress prelaying ducks |
| title | MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks |
| title_full | MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks |
| title_fullStr | MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks |
| title_full_unstemmed | MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks |
| title_short | MiR-106a-5p by Targeting <i>MAP3K2</i> Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks |
| title_sort | mir 106a 5p by targeting i map3k2 i promotes repair of oxidative stress damage to the intestinal barrier in prelaying ducks |
| topic | apla-miR-106a-5p intestinal barrier function MAP3K2 oxidative stress prelaying ducks |
| url | https://www.mdpi.com/2076-2615/14/7/1037 |
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