Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases
RNA-seq has been widely used as a high-throughput method to characterize transcript dynamic changes in a broad context, such as development and diseases. However, whether RNA-seq-estimated transcriptional dynamics can be translated into protein level changes is largely unknown. Ribo-seq (Ribosome pr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/19/2966 |
| _version_ | 1797480099352674304 |
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| author | Atefeh Bagheri Artem Astafev Tara Al-Hashimy Peng Jiang |
| author_facet | Atefeh Bagheri Artem Astafev Tara Al-Hashimy Peng Jiang |
| author_sort | Atefeh Bagheri |
| collection | DOAJ |
| description | RNA-seq has been widely used as a high-throughput method to characterize transcript dynamic changes in a broad context, such as development and diseases. However, whether RNA-seq-estimated transcriptional dynamics can be translated into protein level changes is largely unknown. Ribo-seq (Ribosome profiling) is an emerging technology that allows for the investigation of the translational footprint via profiling ribosome-bounded mRNA fragments. Ribo-seq coupled with RNA-seq will allow us to understand the transcriptional and translational control of the fundamental biological process and human diseases. This review focuses on discussing the principle, workflow, and applications of Ribo-seq to study human diseases. |
| first_indexed | 2024-03-09T21:55:16Z |
| format | Article |
| id | doaj.art-f40f891123444fc9a3868db7911996b5 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T21:55:16Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f40f891123444fc9a3868db7911996b52023-11-23T20:00:18ZengMDPI AGCells2073-44092022-09-011119296610.3390/cells11192966Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human DiseasesAtefeh Bagheri0Artem Astafev1Tara Al-Hashimy2Peng Jiang3Department of Biological, Geological and Environmental Sciences (BGES), Cleveland State University, Cleveland, OH 44115, USADepartment of Biological, Geological and Environmental Sciences (BGES), Cleveland State University, Cleveland, OH 44115, USADepartment of Biological, Geological and Environmental Sciences (BGES), Cleveland State University, Cleveland, OH 44115, USADepartment of Biological, Geological and Environmental Sciences (BGES), Cleveland State University, Cleveland, OH 44115, USARNA-seq has been widely used as a high-throughput method to characterize transcript dynamic changes in a broad context, such as development and diseases. However, whether RNA-seq-estimated transcriptional dynamics can be translated into protein level changes is largely unknown. Ribo-seq (Ribosome profiling) is an emerging technology that allows for the investigation of the translational footprint via profiling ribosome-bounded mRNA fragments. Ribo-seq coupled with RNA-seq will allow us to understand the transcriptional and translational control of the fundamental biological process and human diseases. This review focuses on discussing the principle, workflow, and applications of Ribo-seq to study human diseases.https://www.mdpi.com/2073-4409/11/19/2966ribosome profilingtranslationhuman diseasestranslation efficiency (TE) |
| spellingShingle | Atefeh Bagheri Artem Astafev Tara Al-Hashimy Peng Jiang Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases Cells ribosome profiling translation human diseases translation efficiency (TE) |
| title | Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases |
| title_full | Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases |
| title_fullStr | Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases |
| title_full_unstemmed | Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases |
| title_short | Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases |
| title_sort | tracing translational footprint by ribo seq principle workflow and applications to understand the mechanism of human diseases |
| topic | ribosome profiling translation human diseases translation efficiency (TE) |
| url | https://www.mdpi.com/2073-4409/11/19/2966 |
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