Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of fipronil in suspensions of Parakill. HPLC separation was carried out by reversed phase chromatography on Betasil C18 (250 mm x 4.6 mm i.d.; 5 μm particle size), held in thermostat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Romanian National Association of the Veterinary Products Manufacturers
2011-12-01
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| Series: | Medicamentul Veterinar |
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| Online Access: | http://www.veterinarypharmacon.com/docs/993-2011-ART.%207.%20eng.pdf |
| _version_ | 1819279867352449024 |
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| author | Elena Gabriela Oltean Nica, A. |
| author_facet | Elena Gabriela Oltean Nica, A. |
| author_sort | Elena Gabriela Oltean |
| collection | DOAJ |
| description | An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of fipronil in suspensions of Parakill. HPLC separation was carried out by reversed phase chromatography on Betasil C18 (250 mm x 4.6 mm i.d.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of Acetonitrile/ Distilled water (60/ 40 v/ v), with a flow rate of 1 ml/ min and with UV detection at 220 nm. In order to validate the method, the following parameters have been investigated linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceutical compound in PARAKILL suspensions. |
| first_indexed | 2024-12-24T00:34:43Z |
| format | Article |
| id | doaj.art-f418d848e1d54cb0a5d4472d510e776b |
| institution | Directory Open Access Journal |
| issn | 1843-9527 2069-2463 |
| language | English |
| last_indexed | 2024-12-24T00:34:43Z |
| publishDate | 2011-12-01 |
| publisher | Romanian National Association of the Veterinary Products Manufacturers |
| record_format | Article |
| series | Medicamentul Veterinar |
| spelling | doaj.art-f418d848e1d54cb0a5d4472d510e776b2022-12-21T17:24:07ZengRomanian National Association of the Veterinary Products ManufacturersMedicamentul Veterinar1843-95272069-24632011-12-01526567Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensionsElena Gabriela OlteanNica, A.An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of fipronil in suspensions of Parakill. HPLC separation was carried out by reversed phase chromatography on Betasil C18 (250 mm x 4.6 mm i.d.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of Acetonitrile/ Distilled water (60/ 40 v/ v), with a flow rate of 1 ml/ min and with UV detection at 220 nm. In order to validate the method, the following parameters have been investigated linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceutical compound in PARAKILL suspensions.http://www.veterinarypharmacon.com/docs/993-2011-ART.%207.%20eng.pdffipronilParakillreversed phase high performance liquid chromatography RP–HPLCUV–VIS |
| spellingShingle | Elena Gabriela Oltean Nica, A. Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions Medicamentul Veterinar fipronil Parakill reversed phase high performance liquid chromatography RP–HPLC UV–VIS |
| title | Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions |
| title_full | Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions |
| title_fullStr | Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions |
| title_full_unstemmed | Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions |
| title_short | Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions |
| title_sort | development and validation of a rp hplc method for the quantitation studies of fipronil in parakill suspensions |
| topic | fipronil Parakill reversed phase high performance liquid chromatography RP–HPLC UV–VIS |
| url | http://www.veterinarypharmacon.com/docs/993-2011-ART.%207.%20eng.pdf |
| work_keys_str_mv | AT elenagabrielaoltean developmentandvalidationofarphplcmethodforthequantitationstudiesoffipronilinparakillsuspensions AT nicaa developmentandvalidationofarphplcmethodforthequantitationstudiesoffipronilinparakillsuspensions |