Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection
Background and Aim: Catfish has a high economic value and is popular among consumers. To ensure well-stocked catfish stocks, good fisheries management must also be ensured. The high demand for catfish must be supplemented by preventive measures against pathogenic bacterial infections using probiotic...
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| Format: | Article |
| Language: | English |
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Veterinary World
2024-03-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.17/March-2024/12.pdf |
| _version_ | 1797253743463366656 |
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| author | Nurul Aini Dini Sarafina Yulia Rosa Putri Divany Hunaimatul Achhlam Fatimah Fatimah Sapto Andriyono Dyah Hariani Hoang Dang Khoa Do Sri Puji Astuti Wahyuningsih |
| author_facet | Nurul Aini Dini Sarafina Yulia Rosa Putri Divany Hunaimatul Achhlam Fatimah Fatimah Sapto Andriyono Dyah Hariani Hoang Dang Khoa Do Sri Puji Astuti Wahyuningsih |
| author_sort | Nurul Aini |
| collection | DOAJ |
| description | Background and Aim: Catfish has a high economic value and is popular among consumers. To ensure well-stocked catfish stocks, good fisheries management must also be ensured. The high demand for catfish must be supplemented by preventive measures against pathogenic bacterial infections using probiotics with high potential for Lactobacillus casei and Bacillus subtilis. The aim of this study was to determine the effect of probiotic supplementation consisting of a combination of L. casei and B. subtilis probiotics on the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with Aeromonas hydrophila.
Materials and Methods: This study used a completely randomized study with eight treatments and three replications. The manipulated factor was the probiotic concentration [0% (A), 0.5% (B), 10% (C), and 15% (D)] in groups of catfish infected and uninfected with A. hydrophila. Combination of B. subtilis, and L. casei that were used in a 1:1 ratio of 108 colony forming unit/mL. The study lasted for 42 days. On the 35th day, A. hydrophila was infected by intramuscular injection into fish. The Statistical Package for the Social Sciences (SPSS) software version 23.0 (IBM SPSS Statistics) was used to analyze data on growth, immune system, and water quality.
Results: Providing probiotics in feed can increase the nutritional value of feed based on proximate test results. There were significant differences in average daily gain (ADG), feed conversion ratio (FCR), and survival rate (SR) parameters in the group of catfish infected with A. hydrophila (p > 0.05); however, there were no significant differences in final body weight, specific growth rate (SGR), and percentage weight gain. Interleukin-1β (IL-1β) levels were significantly different between treatments C and D. The tumor necrosis factor (TNF) α parameters were significantly different between treatments A and C, whereas the phagocytic activity of treatment A was significantly different from that of treatment D. There was a significant difference (p > 0.05) in the growth parameters of SGR, ADG, and FCR in the group of fish that were not infected with A. hydrophila, with the best treatment being a probiotic concentration of 15%, but there was no significant difference in the SR parameters. IL-1β and TNF-α levels significantly differed between E and E0 (15% probiotics) but were not significantly different in terms of phagocytosis parameters.
Conclusion: Based on the results of this study, it can be concluded that using a combination of probiotics L. casei and B. subtilis can improve the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with A. hydrophila. |
| first_indexed | 2024-04-24T21:38:55Z |
| format | Article |
| id | doaj.art-f41d980663d94c718d83893ffce839a2 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-24T21:38:55Z |
| publishDate | 2024-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-f41d980663d94c718d83893ffce839a22024-03-21T11:48:56ZengVeterinary WorldVeterinary World0972-89882231-09162024-03-0117360261110.14202/vetworld.2024.602-611Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infectionNurul Aini0https://orcid.org/0009-0003-0685-9908Dini Sarafina Yulia Rosa Putri1https://orcid.org/0009-0008-1658-1934Divany Hunaimatul Achhlam2https://orcid.org/0009-0008-1119-3449Fatimah Fatimah3https://orcid.org/0000-0002-9831-2154Sapto Andriyono4https://orcid.org/0000-0002-2566-1636Dyah Hariani5https://orcid.org/0000-0001-6645-6551Hoang Dang Khoa Do6https://orcid.org/0000-0002-7970-9359Sri Puji Astuti Wahyuningsih7https://orcid.org/0000-0003-1027-7110Doctoral Mathematics and Natural Sciences Study Program, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Department of Agricultural Technology, KH University. A. Wahab Hasbullah, Jombang, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; University Center of Excellence Research Center for Bio-Molecule Engineering, Universitas Airlangga, Surabaya, Indonesia.Department of Marine, Faculty of Fisheries and Marine Sciences, Universitas Airlangga, Surabaya, Indonesia.Department of Biology, Faculty of Mathematics and Natural Sciences, Surabaya State University, Surabaya, Indonesia.NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam.Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.Background and Aim: Catfish has a high economic value and is popular among consumers. To ensure well-stocked catfish stocks, good fisheries management must also be ensured. The high demand for catfish must be supplemented by preventive measures against pathogenic bacterial infections using probiotics with high potential for Lactobacillus casei and Bacillus subtilis. The aim of this study was to determine the effect of probiotic supplementation consisting of a combination of L. casei and B. subtilis probiotics on the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with Aeromonas hydrophila. Materials and Methods: This study used a completely randomized study with eight treatments and three replications. The manipulated factor was the probiotic concentration [0% (A), 0.5% (B), 10% (C), and 15% (D)] in groups of catfish infected and uninfected with A. hydrophila. Combination of B. subtilis, and L. casei that were used in a 1:1 ratio of 108 colony forming unit/mL. The study lasted for 42 days. On the 35th day, A. hydrophila was infected by intramuscular injection into fish. The Statistical Package for the Social Sciences (SPSS) software version 23.0 (IBM SPSS Statistics) was used to analyze data on growth, immune system, and water quality. Results: Providing probiotics in feed can increase the nutritional value of feed based on proximate test results. There were significant differences in average daily gain (ADG), feed conversion ratio (FCR), and survival rate (SR) parameters in the group of catfish infected with A. hydrophila (p > 0.05); however, there were no significant differences in final body weight, specific growth rate (SGR), and percentage weight gain. Interleukin-1β (IL-1β) levels were significantly different between treatments C and D. The tumor necrosis factor (TNF) α parameters were significantly different between treatments A and C, whereas the phagocytic activity of treatment A was significantly different from that of treatment D. There was a significant difference (p > 0.05) in the growth parameters of SGR, ADG, and FCR in the group of fish that were not infected with A. hydrophila, with the best treatment being a probiotic concentration of 15%, but there was no significant difference in the SR parameters. IL-1β and TNF-α levels significantly differed between E and E0 (15% probiotics) but were not significantly different in terms of phagocytosis parameters. Conclusion: Based on the results of this study, it can be concluded that using a combination of probiotics L. casei and B. subtilis can improve the growth, immune system, water quality, proximate value of feed, and body composition of catfish infected with A. hydrophila.https://www.veterinaryworld.org/Vol.17/March-2024/12.pdfcatfishfish stocks and fisheries managementgrowth parametersimmune responseprobiotic |
| spellingShingle | Nurul Aini Dini Sarafina Yulia Rosa Putri Divany Hunaimatul Achhlam Fatimah Fatimah Sapto Andriyono Dyah Hariani Hoang Dang Khoa Do Sri Puji Astuti Wahyuningsih Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection Veterinary World catfish fish stocks and fisheries management growth parameters immune response probiotic |
| title | Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection |
| title_full | Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection |
| title_fullStr | Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection |
| title_full_unstemmed | Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection |
| title_short | Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection |
| title_sort | supplementation of bacillus subtilis and lactobacillus casei to increase growth performance and immune system of catfish clarias gariepinus due to aeromonas hydrophila infection |
| topic | catfish fish stocks and fisheries management growth parameters immune response probiotic |
| url | https://www.veterinaryworld.org/Vol.17/March-2024/12.pdf |
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