Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination
Measles vaccines have been in use since the 1960s with excellent safety and effectiveness profiles. Limited data are available on detection of measles vaccine virus (MeVV) RNA in human subjects following vaccination. Available evidence suggests MeVV RNA can be identified up to 14 days after vaccinat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/7/636 |
| _version_ | 1818570460475949056 |
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| author | Jamie McMahon Ian M Mackay Stephen B Lambert |
| author_facet | Jamie McMahon Ian M Mackay Stephen B Lambert |
| author_sort | Jamie McMahon |
| collection | DOAJ |
| description | Measles vaccines have been in use since the 1960s with excellent safety and effectiveness profiles. Limited data are available on detection of measles vaccine virus (MeVV) RNA in human subjects following vaccination. Available evidence suggests MeVV RNA can be identified up to 14 days after vaccination, with detection beyond this rare. In routine diagnostic testing, we used two real-time reverse transcription-polymerase chain reaction (RT-rPCR) assays targeting M and F genes to identify measles virus (MeV) and MeVV RNA. Confirmatory testing was performed with an N gene RT-rPCR, followed by sequence confirmation of RT-rPCR positives by semi-nested conventional RT-PCR assays targeting portions of the N, H, and L genes. We report detection and confirmation of MeVV RNA from the respiratory tract of 11 children between 100 and 800 days after most recent receipt of measles-containing vaccine. These novel findings emphasize the importance of genotyping all MeV detections and highlight the need for further work to assess whether persistent MeVV RNA represents viable virus and if transmission to close contacts can occur. |
| first_indexed | 2024-12-14T13:41:40Z |
| format | Article |
| id | doaj.art-f42547bd78a74d45b054a3ecde4eeb88 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-14T13:41:40Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f42547bd78a74d45b054a3ecde4eeb882022-12-21T22:59:26ZengMDPI AGViruses1999-49152019-07-0111763610.3390/v11070636v11070636Measles Vaccine Virus RNA in Children More Than 100 Days after VaccinationJamie McMahon0Ian M Mackay1Stephen B Lambert2Public Health Virology Laboratory, Forensic and Scientific Services, 39 Kessels Road, Coopers Plains QLD 4108, AustraliaPublic Health Virology Laboratory, Forensic and Scientific Services, 39 Kessels Road, Coopers Plains QLD 4108, AustraliaChild Health Research Centre, The University of Queensland, 62 Graham Street, South Brisbane QLD 4101, AustraliaMeasles vaccines have been in use since the 1960s with excellent safety and effectiveness profiles. Limited data are available on detection of measles vaccine virus (MeVV) RNA in human subjects following vaccination. Available evidence suggests MeVV RNA can be identified up to 14 days after vaccination, with detection beyond this rare. In routine diagnostic testing, we used two real-time reverse transcription-polymerase chain reaction (RT-rPCR) assays targeting M and F genes to identify measles virus (MeV) and MeVV RNA. Confirmatory testing was performed with an N gene RT-rPCR, followed by sequence confirmation of RT-rPCR positives by semi-nested conventional RT-PCR assays targeting portions of the N, H, and L genes. We report detection and confirmation of MeVV RNA from the respiratory tract of 11 children between 100 and 800 days after most recent receipt of measles-containing vaccine. These novel findings emphasize the importance of genotyping all MeV detections and highlight the need for further work to assess whether persistent MeVV RNA represents viable virus and if transmission to close contacts can occur.https://www.mdpi.com/1999-4915/11/7/636measles vaccinemeasles virusvaccinesmeaslesRT-PCRvaccine safetymeasles-mumps-rubellaRNApersistence |
| spellingShingle | Jamie McMahon Ian M Mackay Stephen B Lambert Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination Viruses measles vaccine measles virus vaccines measles RT-PCR vaccine safety measles-mumps-rubella RNA persistence |
| title | Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination |
| title_full | Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination |
| title_fullStr | Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination |
| title_full_unstemmed | Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination |
| title_short | Measles Vaccine Virus RNA in Children More Than 100 Days after Vaccination |
| title_sort | measles vaccine virus rna in children more than 100 days after vaccination |
| topic | measles vaccine measles virus vaccines measles RT-PCR vaccine safety measles-mumps-rubella RNA persistence |
| url | https://www.mdpi.com/1999-4915/11/7/636 |
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